Play the plug: How bacteria modify recognition by host receptors? DOI Creative Commons
Suma Tiruvayipati, Dharjath S. Hameed,

Niyaz Ahmed

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 14, 2022

The diverse microbial community that colonizes the gastrointestinal tract has remarkable effects on host immune system and physiology resulting in homeostasis or disease. In both scenarios, gut microbiota interacts with their through ligand-receptor binding whereby downstream signaling processes determine outcome of interaction as disease counteractive responses host. Despite several studies microbe-host interactions mechanisms by which this intricate process happens, a comprehensive updated inventory known roles is paramount. ligands originate result to environment contribute either symbiotic parasitic relationships. On other hand, receptors counteract ligand actions mounting neutral an innate response. varying degrees polymorphic changes specificity ligands. Additionally, pathogenic microbes manipulate endogenous enzymes belonging effector protein family. This review focuses diversity similarity microbiome-host health conditions. It thus establishes overview can help identify potential therapeutic targets response critically soaring antimicrobial resistance juxtaposed tardy antibiotic development research.

Language: Английский

Phosphoribosyl-linked serine ubiquitination of USP14 by the SidE family effectors of Legionella excludes p62 from the bacterial phagosome DOI Creative Commons

Jinli Ge,

Ying Wang, Xindi Chen

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(8), P. 112817 - 112817

Published: July 19, 2023

Xenophagy is an evolutionarily conserved host defensive mechanism to eliminate invading microorganisms through autophagic machinery. The intracellular bacterial pathogen Legionella pneumophila can avoid clearance by the xenophagy pathway via actions of multiple Dot/Icm effector proteins. Previous studies have shown that p62, adaptor protein involved in signaling, excluded from Legionella-containing vacuoles (LCVs). Such defects are attributed multifunctional SidE family effectors (SidEs) exhibit classic deubiquitinase (DUB) and phosphoribosyl ubiquitination (PR-ubiquitination) activities, yet remains elusive. In present study, we demonstrate DUB USP14 PR-ubiquitinated SidEs at serine residues, which impairs its activity interactions with p62. exclusion p62 phagosome requires ubiquitin ligase but not SidEs. These results reveal PR-ubiquitination contributes evasion xenophagic L. pneumophila.

Language: Английский

Citations

11
Wolbachia infection confers post-translational modification of glutamic acid decarboxylase and other proteins in D. melanogaster DOI Creative Commons
Sarah Boothman, Sarah Preston, Jonathan S. Minden

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

ABSTRACT Wolbachia pipientis is a ubiquitous intracellular bacterium that known for its manipulation of reproduction in arthropod hosts. has also been shown to colonize virtually all somatic tissues, including the brain, but little about interaction between host and these locations. To this end, we studied effects infection on brain Drosophila melanogaster . Using comparative proteomics, uncovered post-translational modification many proteins within head body upon infection, with glutamic acid decarboxylase being modified only. Given enzyme’s role neurotransmitter synthesis, next tested how impacts behaviors gamma aminobutyric (GABA) production We discovered an improved response yeast odors -infected, mated females compared their uninfected counterparts. Gross measurements GABA whole brains showed no detectable change abundance infection. Treatments antagonist indicated behavioral was not GABA-dependent, leaving mechanism behind -mediated changes behavior obscure. multiple protein propose model which drives several metabolic increase survival specialized niche brain. These results give rise new questions Wolbachia–Drosophila relationship, future work will focus through confers changes. IMPORTANCE In order fully understand biology organism, must interactions resident microbes. commonly used study such interactions, molecular hosts are well understood, especially tissues. Here, address knowledge gap by characterizing Our provide first description modifications induced host, unveiling level regulation –host relationship. The be connected or behavior, indicating another enzyme during Altogether, more information ’s tissue spark inquiries into host–bacterium

Language: Английский

Citations

0
Ubiquitin and its relatives as wizards of the endolysosomal system DOI Creative Commons
Ilana Berlin, Ayşegül Sapmaz, Virginie Stévenin

et al.

Journal of Cell Science, Journal Year: 2023, Volume and Issue: 136(4)

Published: Feb. 15, 2023

ABSTRACT The endolysosomal system comprises a dynamic constellation of vesicles working together to sense and interpret environmental cues facilitate homeostasis. Integrating extracellular information with the internal affairs cell requires endosomes lysosomes be proficient in decision-making: fusion or fission; recycling degradation; fast transport contacts other organelles. To effectively discriminate between these options, employs complex regulatory strategies that crucially rely on reversible post-translational modifications (PTMs) ubiquitin (Ub) ubiquitin-like (Ubl) proteins. cycle conjugation, recognition removal different Ub- Ubl-modified states informs cellular protein stability behavior at spatial temporal resolution is thus well suited finetune macromolecular assembly function membranes. Here, we discuss how ubiquitylation (also known as ubiquitination) its biochemical relatives orchestrate endocytic traffic designate cargo fate, influence membrane identity transitions support formation contact sites (MCSs). Finally, explore opportunistic hijacking Ub Ubl modification cascades by intracellular bacteria remodel host trafficking pathways invade prosper inside cells.

Language: Английский

Citations

8
An Emerging Way for Bacteria to Engage with Host Cells via Protein ADP-riboxanation DOI Creative Commons
Wei Xian, Zhiheng Tang, Qinxin Zhang

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(11), P. 467 - 467

Published: Nov. 1, 2024

Post-translational modifications (PTMs) are increasingly recognized as important strategies used by bacterial pathogens to modulate host cellular functions. Protein ADP-riboxanation, a derivative of ADP-ribosylation, has recently emerged new biochemical way which interact with cells. Recent studies have revealed that this modification broad regulatory roles in processes including cell death, protein translation, and stress granule formation. Given the vast majority ADP-riboxanases still uncharacterized, review we also highlight utility advanced proteomic tools functional dissection ADP-riboxanation events during infections.

Language: Английский

Citations

0
AMPylation of small GTPases by Fic enzymes DOI Creative Commons
Burak Gulen, Amanda K. Casey, Kim Orth

et al.

FEBS Letters, Journal Year: 2022, Volume and Issue: 597(6), P. 883 - 891

Published: Oct. 14, 2022

Small GTPases orchestrate numerous cellular pathways, acting as molecular switches and regulatory hubs to transmit signals because of this, they are often the target pathogens. During infection, pathogens manipulate host networks using post-translational modifications (PTMs). AMPylation, modification proteins with AMP, has been identified a common PTM utilized by hijack GTPase signalling during infection. AMPylation is primarily carried out enzymes filamentation induced cyclic-AMP (Fic) domain. Modification small AMP renders impervious upstream inputs, resulting in unregulated downstream effector outputs for processes. Here, we overview Fic-mediated other related PTMs catalysed Fic on GTPases.

Language: Английский

Citations

2
Play the plug: How bacteria modify recognition by host receptors? DOI Creative Commons
Suma Tiruvayipati, Dharjath S. Hameed,

Niyaz Ahmed

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 14, 2022

The diverse microbial community that colonizes the gastrointestinal tract has remarkable effects on host immune system and physiology resulting in homeostasis or disease. In both scenarios, gut microbiota interacts with their through ligand-receptor binding whereby downstream signaling processes determine outcome of interaction as disease counteractive responses host. Despite several studies microbe-host interactions mechanisms by which this intricate process happens, a comprehensive updated inventory known roles is paramount. ligands originate result to environment contribute either symbiotic parasitic relationships. On other hand, receptors counteract ligand actions mounting neutral an innate response. varying degrees polymorphic changes specificity ligands. Additionally, pathogenic microbes manipulate endogenous enzymes belonging effector protein family. This review focuses diversity similarity microbiome-host health conditions. It thus establishes overview can help identify potential therapeutic targets response critically soaring antimicrobial resistance juxtaposed tardy antibiotic development research.

Language: Английский

Citations

0