Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo

Journal of Nanobiotechnology, Journal Year: 2022, Volume and Issue: 20(1)

Published: March 19, 2022

Abstract Background Silica nanoparticles (SiO 2 NPs) are extensively applied in the biomedical field. The increasing medical application of SiO NPs has raised concerns about their safety. However, studies on NP-induced retinal toxicity lacking. Methods We investigated with different sizes (15 and 50 nm) vitro vivo along underlying mechanisms. cytotoxicity was assessed R28 human precursor cells by determining ATP content LDH release. cell morphologies nanoparticle distributions were analyzed phase-contrast microscopy transmission electron microscopy, respectively. mitochondrial membrane potential examined confocal laser scanning microscopy. induced immunohistochemical analysis. To further investigate mechanism NPs, reactive oxygen species (ROS) generation, glial activation inflammation monitored. Results 15-nm found to have higher than larger NPs. Notably, vivo, as demonstrated increased death retina, TUNEL-stained cells, ganglion degeneration, activation, inflammation. In addition, caused oxidative stress, increase ROS indicator H DCF-DA. Furthermore, pretreatment N-acetylcysteine, an scavenger, attenuated production Conclusions These results provide evidence that induce size-dependent suggest generation contribute this toxicity. Graphical

Language: Английский

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Pulmonary Toxicity of Silica Linked to Its Micro- or Nanometric Particle Size and Crystal Structure: A Review

Nanomaterials, Journal Year: 2022, Volume and Issue: 12(14), P. 2392 - 2392

Published: July 13, 2022

Silicon dioxide (SiO2) is a mineral compound present in the Earth’s crust two forms: crystalline and amorphous. Based on epidemiological and/or biological evidence, pulmonary effects of silica are considered well understood, with development silicosis, emphysema, chronic bronchitis, or obstructive disease. The structure capacity to trigger oxidative stress recognized as relevant determinants silica’s toxicity. In contrast, natural amorphous was long nontoxic, often used negative control experimental studies. However, manufactured nanoparticles (or nanosilica SiNP) becoming widely industrial applications, these paradigms must now be reconsidered at nanoscale (<100 nm). Indeed, recent studies appear point towards significant toxicity similar that micrometric silica. this article, we an extensive review nontumoral based vitro vivo findings presented both for micro- particles, but also particles.

Language: Английский

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In vitro wheat protoplast cytotoxicity of polystyrene nanoplastics

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 882, P. 163560 - 163560

Published: April 18, 2023

Language: Английский

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Epithelium-derived exosomes promote silica nanoparticles-induced pulmonary fibroblast activation and collagen deposition via modulating fibrotic signaling pathways and their epigenetic regulations

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: June 12, 2024

Abstract Background In the context of increasing exposure to silica nanoparticles (SiNPs) and ensuing respiratory health risks, emerging evidence has suggested that SiNPs can cause a series pathological lung injuries, including fibrotic lesions. However, underlying mediators in fibrogenesis caused by have not yet been elucidated. Results The vivo investigation verified long-term inhalation induced fibroblast activation collagen deposition rat lungs . vitro, uptake exosomes derived from SiNPs-stimulated epithelial cells (BEAS-2B) fibroblasts (MRC-5) enhanced its proliferation, adhesion, activation. particular, mechanistic revealed stimulated an increase epithelium-secreted exosomal miR-494-3p thereby disrupted TGF-β/BMPR2/Smad pathway via targeting bone morphogenetic protein receptor 2 (BMPR2), ultimately resulting deposition. Conversely, inhibitor exosomes, GW4869, abolish induction upregulated MRC-5 SiNPs-treated supernatants BEAS-2B. Besides, inhibiting or overexpression BMPR2 could ameliorate interfering with pathway. Conclusions Our data pulmonary epithelium-derived serve essential role upon stimuli, attributing modulate Hence, strategies be new avenue developing therapeutics against injury elicited SiNPs. Graphical

Language: Английский

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Ultraviolet-induced photodegradation elevated the toxicity of polystyrene nanoplastics on human lung epithelial A549 cells

Environmental Science Nano, Journal Year: 2021, Volume and Issue: 8(9), P. 2660 - 2675

Published: Jan. 1, 2021

UV-induced photodegradation posed greater cytotoxicity, as clearly evidenced by the impaired cell viability, stronger oxidative stress, serious membrane damage, intensive mitochondrial dysfunction, and altered transcriptome responses.

Language: Английский

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Silica nanoparticles perturbed mitochondrial dynamics and induced myocardial apoptosis via PKA-DRP1-mitochondrial fission signaling

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 842, P. 156854 - 156854

Published: June 21, 2022

Language: Английский

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Silica nanoparticles induce pulmonary autophagy dysfunction and epithelial-to-mesenchymal transition via p62/NF-κB signaling pathway

Ecotoxicology and Environmental Safety, Journal Year: 2022, Volume and Issue: 232, P. 113303 - 113303

Published: Feb. 11, 2022

It has been reported that silica nanoparticles (SiNPs) could cause epithelial-to-mesenchymal transition (EMT), but the specific mechanism is still unclear. Thus, purpose of this study was to investigate underlying mechanisms pulmonary EMT after subacute exposure SiNPs. The results showed intratracheal instillation SiNPs increased MDA content, while decreased activity SOD and GSH-Px in rats. Western blot analysis demonstrated induced autophagy dysfunction via upregulation p62. Meanwhile, inflammation cytokines (TNF-α, IL-18, IL-1β) were released rat lung. Immunohistochemistry western assays both regulate related protein biomarkers through decreasing E-cadherin increasing vimentin a dose-dependent manner. Besides, activated proteins expression involved p62/NF-κB signaling pathway, whereas by significantly dampened knock down In study, we illustrated trigger inflammation, further lead activating pathway. Our findings provide new molecular evidence for SiNPs-induced toxicity.

Language: Английский

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Proteomics revealed composition- and size-related regulators for hepatic impairments induced by silica nanoparticles

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 922, P. 170584 - 170584

Published: Feb. 2, 2024

Language: Английский

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High biocompatible FITC-conjugated silica nanoparticles for cell labeling in both in vitro and in vivo models

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 23, 2024

Abstract Fluorescence nanosilica-based cell tracker has been explored and applied in biological research. However, the aggregation of these nanoparticles at physiological pH is still main limitation. In this research, we introduced a novel fluorescence nano-based suitable for application live cells. The silica-coated fluorescein isothiocyanate isomer (FITC-SiO 2 ) (NPs) were modified with carboxymethylsilanetriol disodium salt -COOH), integrating dianion form FITC molecules. This nanosystem exhibited superior dispersion aqueous solutions effectively mitigated dye leakage. These labeled NPs displayed notable biocompatibility minimal cytotoxicity both vitro vivo conditions. Significantly, did not have negative implications on migration or angiogenesis. They successfully penetrated primary fibroblasts, human umbilical vein endothelial cells HeLa 2D 3D cultures, signal enduring over 72 h. Furthermore, NP signals consistently observed developing gastrointestinal tract medaka fish larvae extended periods during phases subdued digestive activity, without manifesting any apparent acute toxicity. results underscore promising utility FITC-SiO -COOH as advanced trackers

Language: Английский

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Integrated non-targeted lipidomics and metabolomics analyses for fluctuations of neonicotinoids imidacloprid and acetamiprid on Neuro-2a cells

Environmental Pollution, Journal Year: 2021, Volume and Issue: 284, P. 117327 - 117327

Published: May 7, 2021

Language: Английский

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