Response to letter to the editor about Rosales-Pérez et al., 2022 (https://doi.org/10.1016/j.chemosphere.2022.133791)instigating reflections on methodological and analytical rigor in ecotoxicological studies based on the research by Rosales-Pérez et al. (2022) by Guilherme Malafaia

Chemosphere, Journal Year: 2022, Volume and Issue: 312, P. 137128 - 137128

Published: Nov. 9, 2022

Language: Английский

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Neurotoxic Cocktail of Low-Dose Metals: Investigating the Impact of Environmentally Relevant Lead, Cadmium, Mercury and Arsenic Mixture in Vivo and in Silico

Published: Jan. 1, 2023

The assessment of neurotoxic effects lead, cadmium, mercury, and arsenic mixture (MIX) administered at environmentally relevant low doses, was conducted using both in vivo silico approaches. A subacute study on a rat model (number animals per group: 5), consisting control five treatment groups subjected to oral exposure with gradually increasing doses (from MIX 1 5). Behavioral tests were the last day, brain tissue examined for oxidative stress, pathohistological changes acetylcholinesterase (AChE) activity. Low did not affect spontaneous locomotor activity (SLA), but highest dose showed tendency memory impairment (NORT). Dose-response analysis indicated that even concentrations toxic metals can cause damage (Benchmark methodology). 2 imbalance most narrow BMDI detected superoxide dismutase falling within range 1e-06 3.18e-05 mg As/kg b.w./day. Pathohistological alterations severe 5 group. In (Comparative Toxicogenomics Database) predicted reduction AChE by MIX. This prediction subsequently validated an experiment, demonstrating dose-dependent decrease. current highlights vulnerability mature minimal amounts investigated metal mixture, particularly context early onset cholinergic susceptibility higher doses.

Language: Английский

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The use of zebrafish to assess water quality and remediation efforts.

Published: Jan. 1, 2023

Although wastewater effluents continue to be significant polluters of aquatic ecosystems in developing countries with limited water resources, little is known about the ecotoxicity induced by these on fish throughout their early life stages. Several treatment plants (WWTPs) South Africa (SA) do not adequately meet minimal requirements established country's Department Water and Sanitation (DWS). Moreover, contaminants emerging concern (CECs) originating from synthetic or natural sources, are widely distributed environments SA. This includes a broad range chemical compounds, such as aspirin (44243 ng/L), Fluoroquinolones (27100 Atenolol (25900 Nalidixic acid (25234 ng/L) Ciprofloxacin (20514 ng/L). In addition endocrine disrupting chemicals, pharmaceuticals personal care products also systems. process treatment, agents flocculants, coagulants, precipitants (e.g., calcium hydroxide sodium hydroxide) chlorine disinfectants utilized settings. However, research understand adverse effects that can caused organisms still ongoing order bridge this knowledge gap, advanced techniques could employed help reveal well any shortcomings current remediation techniques. Using an appropriate model organism highly conserved physiological pathways present higher vertebrates (including humans), rich behavioural repertoire, occurrence variety habitats would novel approach. To effect, study zebrafish aim monitor six distinct samples various regions SA assess effectiveness currently used chlorination. Two effluents, namely, Southern Works Final Effluents (SWFE) Jacob’s Incoming (JB) alerted potential toxicity during characterization suboptimal pH (SWFE = 9.02 ± 0.16 JB 5.65 0.02) total alkalinity zero (0 mg/L) detected for both effluents. The lethal was seen elevation mortality rate up 77 2.89 % 100 0.00 %, respectively SWFE at 40 corresponding LC50 values 17.77 16.46 %. jaw face, heart, brain, fins, notochord, somite tail were significantly deformed (p < 0.05) post-exposure revealed morphological scores upon analysis zebrafish’s body structure. there delay development due aforementioned unsuccessful hatching, craniofacial abnormalities, pericardial yolk sac oedema, notochord abnormality defects spinal cord curvature. addition, locomotor activity inhibited following observation distance travelled, frozen moments, acceleration rates, swimming trajectories exploration rate. Surprisingly, safety wastewaters restored precipitation revealing non-lethal ranges 6.02 - 8.02 6.65 7.65 JB, reducing non-significant levels > compared control. Also, bicarbonate (NaHCO3) 120 mg/L found effective supplementing alkalinity. contrast, Amanzimtoti before after chlorination (TB TA), Badulla (IB) Chatsworth (CI) exhibited no consistent lethality apparent stress demonstrated insignificant expression protein: heat shock protein 70 (HSP70). tested (TB) (TA) appeared same (~25 %) indicating enough completely remediating wastewater. Our pioneer evaluating ecotoxicological impact localized country like relation adjustment quality parameters neutralization contaminants. better released SA's bodies interactions adult stage, more studies needs developed.

Language: Английский

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Instigating reflections on methodological and analytical rigor in ecotoxicological studies based on the research by Rosales-Pérez et al. (2022)

Chemosphere, Journal Year: 2022, Volume and Issue: 309, P. 136555 - 136555

Published: Sept. 30, 2022

Language: Английский

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