Co-fermentation of dark septate endophytes and Trichoderma viride can effectively degrade cellulose in different Chinese medicinal herbal residues

International Biodeterioration & Biodegradation, Journal Year: 2024, Volume and Issue: 196, P. 105949 - 105949

Published: Nov. 5, 2024

Language: Английский

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Cultivar-specific dynamics: unravelling rhizosphere microbiome responses to water deficit stress in potato cultivars

BMC Microbiology, Journal Year: 2023, Volume and Issue: 23(1)

Published: Dec. 1, 2023

Abstract Background Growing evidence suggests that soil microbes can improve plant fitness under drought. However, in potato, the world’s most important non-cereal crop, role of rhizosphere microbiome drought has been poorly studied. Using a cultivation independent metabarcoding approach, we examined two potato cultivars with different tolerance as function water regime (continuous versus reduced watering) and manipulation microbial diversity (i.e., natural (NSM), vs. disturbed (DSM) microbiome). Results Water pre-treatment showed significant interaction bacterial community composition sensitive (HERBST) but not resistant cultivar (MONI). Overall, MONI had moderate response to treatments its selected Rhizobiales watering NSM soil, whereas Bradyrhizobium , Ammoniphilus Symbiobacterium unclassified Hydrogenedensaceae DSM soil. In contrast, HERBST was more pronounced. Notably, treated watering, root endophytic fungus Falciphora many Actinobacteriota members ( Streptomyces Glycomyces Marmoricola Aeromicrobium Mycobacterium others) were largely represented. treatment resulted no fungal taxa fewer enrichment these watering. Moreover, number core amplicon sequence variants (core ASVs) consistent regardless regimes opposed HERBST, which marked reduction ASVs observed Conclusions Besides influence conditions, our results indicate strong cultivar-dependent relationship between their capacity respond perturbations such moisture. Our study highlights importance integrating conditions genetic variability key factors future breeding programs aiming develop resistance major food crop like potato. Elucidating molecular mechanisms how plants recruit from help mitigate stress identify taxa, harbour respective traits might therefore be an topic for research.

Language: Английский

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Apple Root Microbiome as Indicator of Plant Adaptation to Apple Replant Diseased Soils

Microorganisms, Journal Year: 2023, Volume and Issue: 11(6), P. 1372 - 1372

Published: May 24, 2023

The tree fruit industry in Nova Scotia, Canada, is dominated by the apple (Malus domestica) sector. However, sector faced with numerous challenges, including replant disease (ARD), which a well-known problem areas intensive cultivation. A study was performed using 16S rRNA/18S rRNA and rRNA/ITS2 amplicon sequencing to assess soil- root-associated microbiomes, respectively, from mature orchards soil microbiomes alone uncultivated soil. results indicated significant (p < 0.05) differences microbial community structure composition between cultivated orchard We identified an increase number of potential pathogens compared At same time, we detected relative abundances several plant-growth-promoting or biocontrol microorganisms non-fungal eukaryotes capable promoting proliferation bacterial agents soils. Additionally, roots accumulated PGP bacteria Proteobacteria Actinobacteria phyla, while fungal taxa contribute ARD, such as Nectriaceae plant pathogenic Fusarium spp., were decreased root microbiome microbiome. suggest that health can be ascribed complex interaction growth-promoting on roots.

Language: Английский

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Morphogenesis and metabolomics reveal the compatible relationship among Suillus bovinus , Phialocephala fortinii , and their co-host, Pinus massoniana

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(5)

Published: Sept. 7, 2023

ABSTRACT Ectomycorrhizal (ECM) fungi and dark septate endophytes (DSEs) can both form a symbiotic relationship with the same host plant. However, interactions that occur among these two types of their co-hosts are largely unknown. Here, we investigated ECM fungus Suillus bovinus , DSE Phialocephala fortinii co-host Pinus massoniana . We used scanning electron microscopy optical to characterize morphogenesis symbionts employed ultra-high-performance liquid chromatography–tandem mass spectrometry technique assess effects fungal inoculation on root metabolome. Under pure culture conditions, no synergistic or antagonistic were observed between Phi. S. Generally, simultaneously colonize P. roots without affecting each other’s processes. locus where has already invaded but not vice versa, which may be due physical barrier effect mantle. Both significantly promote growth they have N K uptake. Metabolite accumulation patterns in inoculated and/or greatly altered, especially respect organic acids, flavonoids, lipids, phenolic acids. enhanced flavonoid biosynthesis, whereas dual-inoculation treatments mainly induced phenylpropanoid biosynthesis. These findings reveal compatible relationships suggest theoretical basis for co-application when cultivating seedlings. IMPORTANCE The prevalence ectomycorrhizal wide spectrum tree species is well recognized. In this study, co-host, appears superior microniche competition, different metabolite patterns. This first study revealing morphological metabolic mechanisms contribute fungi, DSEs, co-host.

Language: Английский

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Fern mycorrhizae do not respond to fertilization in a tropical montane forest

Plant-Environment Interactions, Journal Year: 2024, Volume and Issue: 5(2)

Published: March 29, 2024

Abstract Ferns are known to have a lower incidence of mycorrhization than angiosperms. It has been suggested that this results from carbon being more limiting fern growth nutrient availability, but assertion not tested yet. In the present study, we took advantage fertilization experiment with nitrogen and phosphorus on cloud forest plots Ecuadorean Andes for 15 years. A previous analysis revealed changes in abundances species fertilized compared control hypothesized might be related responses mycorrhizal relationships availability. We revisited assess root‐associated fungal communities two epiphytic terrestrial showed shifts abundance. sampled analyzed roots 125 individuals following metabarcoding approach. recovered 1382 ASVs, dominance members Tremellales (Basidiomycota) Heliotales (Ascomycota). The diversity was highly partitioned little overlap between individuals. found marked differences species, latter fundamentally missing arbuscular fungi (AMF). no effect or relative abundance assemblages. Still, observed direct impact its concentration leaves. conclude fern–fungi study site restricted by availability suggest existence specificity partners host species.

Language: Английский

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