Riverine antibiotic resistome along an anthropogenic gradient

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

The introduction of antibiotic-resistant bacteria into riverine systems through the discharge wastewater treatment plant (WWTP) effluent and agricultural waste poses significant health risks. Even when not pathogenic, these can act as reservoirs for antibiotic resistance genes (ARGs), transferring them to pathogens that infect humans animals. In this study, we used fluorescence in situ hybridization, qPCR, metagenomics investigate how anthropogenic activities affect microbial abundance resistome along Holtemme River, a small river Germany, from near-pristine human-impacted sites. Our results showed higher bacterial abundance, greater absolute relative ARGs, more diverse ARG profile at impacted Overall, profiles sites reflected usage with conferring drug classes such beta-lactams, aminoglycosides, folate biosynthesis inhibitors, tetracyclines. There were also variations Notably, there was high oxacillin gene OXA-4 downstream site river. metagenome assembly, associated contig homologous plasmids previously identified members Thiotrichaceae. likely in-situ host putative plasmid close Thiolinea (also known Thiothrix) eikelboomii, prominent member WWTP microbiomes worldwide. show WWTPs introduce environment shuttle clinically relevant ARG.

Language: Английский

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Implications on freshwater lake-river ecosystem protection suggested by organic micropollutant (OMP) priority list

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 461, P. 132580 - 132580

Published: Sept. 19, 2023

Language: Английский

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Anthropogenic pollution may enhance natural transformation in water, favouring the spread of antibiotic resistance genes

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 475, P. 134885 - 134885

Published: June 12, 2024

Language: Английский

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Integrons in the Age of Antibiotic Resistance: Evolution, Mechanisms, and Environmental Implications: A Review

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2579 - 2579

Published: Dec. 13, 2024

Integrons, which are genetic components commonly found in bacteria, possess the remarkable capacity to capture gene cassettes, incorporate them into their structure, and thereby contribute an increase genomic complexity phenotypic diversity. This adaptive mechanism allows integrons play a significant role acquiring, expressing, spreading antibiotic resistance genes modern age. To assess current challenges posed by integrons, it is necessary have thorough understanding of characteristics. review aims elucidate structure evolutionary history highlighting how use antibiotics has led preferential selection various environments. Additionally, explores involvement dissemination across diverse settings, while considering potential transmission factors routes. delves arrangement cassettes within ability rearrange, mechanisms governing expression, process excision. Furthermore, this study examines presence clinically relevant wide range environmental sources, shedding light on anthropogenic influences propagation environment.

Language: Английский

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Intensified anthropogenic disturbances impair planktonic algae in an urban river

Journal of Cleaner Production, Journal Year: 2024, Volume and Issue: 468, P. 143091 - 143091

Published: July 6, 2024

Language: Английский

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One Health Threat of Treated Wastewater Discharge in Urban Ohio Rivers: Implications for Surface Water and Fish Gut Microbiome and Resistome

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: July 16, 2024

Wastewater treatment plants (WWTPs) are thought to be a major disseminating source of antibiotic resistance (AR) the environment, establishing crucial connection between human and environmental resistome. The objectives this study were determine how wastewater effluents impact microbiome resistome freshwater fish, identify potential AR-carrying clinically relevant pathogens in these matrices. We analyzed influent effluent from four WWTPs three metropolitan areas Ohio, USA via shotgun metagenomic sequencing. also sequenced river water fish guts reaches (upstream, at WWTP outfall, downstream). Notably, we observed decline diversity AR gene abundance receiving river. found significant differences by reach trophic level (diet) beta-diversity gut microbiomes. SourceTracker revealed that 0.443 0.248 more was sourced outfall downstream locations, respectively, compared upstream fish. Additionally, bacteria public health concern annotated samples, indicating for exposure. In summary, our findings show continued role as reservoir underscores considerable discharge on aquatic wildlife, which highlights One Health nature issue.

Language: Английский

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Sinking Particles as Repository of Antimicrobial Resistome Diversity in the Sansha Yongle Blue Hole

Published: Jan. 1, 2025

The aquatic environment plays a central role in the selection and spread of antimicrobial resistance genes (ARGs). Using metagenomic approaches, several studies have provided comprehensive view resistome across different ecosystems. However, unique systems, such as oceanic blue holes, remain largely unexplored. In this study, free-living particle-associated shotgun metagenome sequencing data, collected Sansha Yongle Blue Hole (South China Sea) water column, were analyzed to reveal (total content ARGs within defined bacterial community) potential for horizontal gene transfer detected ARGs. diversity overall significantly decreased with depth, although trend was driven by community, while it remained stable communities. Additionally, latter showed higher frequency co-occurring mobile genetic elements on same contigs, frequent plasmid localization these genes. Overall, bacteria proved essential sustaining diversity. Furthermore, findings suggest potentially rate ARG microbial communities along column ensuring persistence resistances deep marine waters.

Language: Английский

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Using A Harmonised Study Design and Quantitative Toolbox Reveals Major Inconsistencies When Investigating the Main Drivers of Water and Biofilm Antibiotic Resistomes in Different Rivers

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 137343 - 137343

Published: Jan. 1, 2025

Language: Английский

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Environmental drivers of the resistome across the Baltic Sea

Microbiome, Journal Year: 2025, Volume and Issue: 13(1)

Published: April 7, 2025

Abstract Background Antimicrobial resistance is a major global health concern, with the environment playing key role in its emergence and spread. Understanding relationships between environmental factors, microbial communities, mechanisms vital for elucidating resistome dynamics. In this study, we characterized of Baltic Sea evaluated how gradients spatial variability, alongside communities associated functional genes, influence diversity composition across geographic regions. Results We analyzed metagenomes benthic sediments from 59 monitoring stations 1,150 km distance Sea, revealing an comprised predicted antimicrobial genes (ARGs) against 26 antibiotic classes. observed variation profile, higher northern regions decline dead zones southern areas. The combined effects salinity temperature gradients, nutrient availability, created complex landscape that shaped distribution ARGs. Salinity predominantly influenced ARG composition, leading to clear distinctions high-saline those lower mid-level salinity. Furthermore, our analysis suggests community mobile genetic elements might be crucial shaping composition. Conclusions presented were identified as primary factors influencing regions, availability further these patterns Sea. Our study also highlighted interplay resistance, ecosystem, underscoring potential element distribution. modulate resistomes will help predict impact future changes on aquatic ecosystems.

Language: Английский

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Distribution status and influencing factors of antibiotic resistance genes in the Chaohu Lake, China

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19384 - e19384

Published: April 25, 2025

Background Chaohu Lake (CL) is one of the most polluted areas in China due to its high content antibiotics. However, distribution and influencing factors antibiotic resistance genes (ARGs) this lake are still controversial. Methods To solve problem, we used metagenomic sequencing investigate in-fluencing ARGs CL. Results Our findings revealed existence nine kinds ARGs, including 45 specific genes. The abundant types were multidrug, bacitracin, polymyxin, macrolide lincosamide streptogramin, aminoglycoside. Multiple microorganisms undeniable ARG reservoirs, although they not dominant species microbiota. results also showed that both microbiota physiochemical played important roles shaping distributions Specifically, levels PO4-P (0.5927) total phosphorus (0.4971) had a greater impact than nitrogen (0.0515), NO 3- N (0.0352), NO2-N (−0.1975), NH3-N (−0.0952). Conclusions These provide valuable insights into lakes.

Language: Английский

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