
International Journal of Microbiology, Journal Year: 2024, Volume and Issue: 2024(1)
Published: Jan. 1, 2024
The goal of the study is to evaluate metabolic and morphological changes facultative alkaliphile Bacillus aequororis 5‐DB weakly alkali‐resistant B. subtilis ATCC 6633 in a wide pH range at different NaCl concentrations. shown have broader general resistance adverse factors (wide range, 50 g/L NaCl) than alkali‐tolerant strain same genus. This also significantly greater not only high but low comparison with 6633. was expressed higher activity, maintenance ΔpH, no significant cell damage. selected set methods (reduction resazurin resorufin by dehydrogenases, bioluminescent method for determining ATP, AFM, measurement intracellular pH) allows us adequately assess ability microbial cells withstand harsh environmental factors. Nonspecific proven using complex methods. Tolerance salt concentrations may be useful biotechnological applications strain.
Language: Английский