Analytical methodology for unveiling human exposure to (micro)plastic additives

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 173, P. 117653 - 117653

Published: March 12, 2024

This review describes a wide variety of analytical approaches for the assessment human exposure to organic chemicals associated with plastic additives, focusing on works published in last decade plasticisers, bisphenols, flame retardants and antioxidants. Physiologically based extraction tests serve as preliminary in-vitro assays determine bioaccessibility these compounds from micro/nanoplastics body fluids gastrointestinal tract, skin, or lung. Whenever plastic-laden become bioavailable, metabolism is be monitored through phase I II metabolites. In this regard, methods chromatography mass spectrometry biomonitoring parent their metabolites biological samples (mostly urine plasma) are discussed depth. also covers role wastewater-based epidemiology determining overall given population plastic-related species.

Language: Английский

Electromembrane Extraction in Suspect Screening of Polar Organic Xenobiotics and their Metabolites in Human Urine: A New Approach to Enhance Compound Annotation?

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

Suspect and nontarget screening (SNTS) methodologies using human urine are invaluable strategies for understanding the exposome. However, very polar organic compounds often overlooked in those methods due to challenges sample preparation chromatographic analysis. Although "dilute-and-shoot" (DS) followed by mixed-mode liquid chromatography-high-resolution mass spectrometry (MMLC-HRMS) might be deemed suitable, complementary needed enhance SNTS expand compound identification. In this context, potential of nonsupported microelectromembrane extraction (μ-EME) is thoroughly studied as a supplement DS-MMLC-HRMS. It was demonstrated that μ-EME-MMLC-HRMS enables refinement suspect results from 24 h pooled sample. The selective capability μ-EME charged analytes, compared DS, allowed identification false positives 4 negatives. confidence level 6 suspects also enhanced through interpretation. Moreover, nine were identified exclusively experiments cleanup provided technique. Notably, containing carboxylic acid groups (phase II metabolites) amines particularly well-annotated employing conditions acids bases, respectively. Thus, proves confirmatory dimension MMLC-based approaches.

Language: Английский

Characterization of Chemical Exposome in A Paired Human Preconception Pilot Study

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(46), P. 20352 - 20365

Published: Nov. 7, 2024

Parental preconception exposure to synthetic chemicals may have critical influences on fertility and reproduction. Here, we present a robust LC-MS/MS method covering up 95 diverse xenobiotics in human urine, serum, seminal follicular fluids support exposome-wide assessment reproductive health outcomes. Extraction recoveries of validated analytes ranged from 62% 137% limits quantification 0.01 6.0 ng/mL all biofluids. We applied the cohort Australian couples (

Language: Английский