Enhanced Anaerobic Digestion Performance Through Acyl Homoserine Lactone-Mediated Quorum Sensing and Supplemental Voltage Regulation

Fermentation, Journal Year: 2025, Volume and Issue: 11(3), P. 117 - 117

Published: March 2, 2025

Traditional anaerobic digestion (AD) technology continues to have severe limitations in terms of complicated substrate degradation efficiency and methane production. This study optimizes the AD system using corn straw cattle manure as substrates by introducing an exogenous N-Hexanoyl-L-Homoserine lactone (C6-HSL) signaling molecule concert with applied external voltage 0.8 V, systematically investigating its impact on methanogenic performance microbial community dynamics. The results show that combined regulation significantly increased production (by 29.74%) utilization rate 74.73%) while preventing acid inhibition ammonia nitrogen inhibition. Mechanistic analysis revealed enhanced system’s electrocatalytic activity, C6-HSL further facilitated electron transfer biofilm electrode. notably enriched hydrogenotrophic methanogens (with Methanobacterium predominating cathode Methanobrevibacter digestate), establishing a stable metabolic cooperative network both electrode digestate, optimizing methanogenesis pathway, enhancing synergistic effects among communities robustness. uncovers enhancement mechanism voltage, providing new technological pathways theoretical support for efficient conversion low-quality biomass resources clean energy.

Language: Английский

Enzymes targeting distinct hydrolysis blind-spots of thermal and biological pre-treatments significantly uplift biogas production

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132353 - 132353

Published: March 1, 2025

Thermal hydrolysis process (THP) and biological (BH) are key pre-treatment technologies for anaerobic digestion (AD), termed advanced digesters (AADs). They target the rate-limiting step in AD. This study evaluates full-scale pre-treatments macromolecule bias implementation of enzymes to enhance biogas yield. Findings show THP significantly improves protein carbohydrate solubilisation by 30% 25%, respectively, but fully hydrolyses only carbohydrates. In contrast, BH targets fibres proteins, achieving 35% 23% solubilisation, partially Biomethane potential (BMP) tests indicate that protease raise biomethane yield 20-30% AAD with pre-treatment. comparison, α-amylase increases it over tailors enzyme selection dosage specifically address unique "hydrolysis blind spot" each pre-treatment, providing a strategic framework AD an improved understanding selectivity their transformation pathways.

Language: Английский

Quorum sensing in anaerobic wastewater treatment: Potential microbiological mechanisms and enhancement strategies

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 156150 - 156150

Published: Sept. 1, 2024

Language: Английский