A Biorefinery Approach Integrating Lipid and EPS Augmentation Along with Cr (III) Mitigation by Chlorella minutissima DOI Creative Commons

Sonia Choudhary,

Mansi Tiwari, Krishna Mohan Poluri

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2047 - 2047

Published: Dec. 11, 2024

The quest for cleaner and sustainable energy sources is crucial, considering the current scenario of a steep rise in consumption fuel crisis, exacerbated by diminishing fossil reserves rising pollutants. In particular, bioaccumulation hazardous substances like trivalent chromium has not only disrupted fragile equilibrium ecological system but also poses significant health hazards to humans. Microalgae emerged as promising solution achieving sustainability due their ability remediate contaminants produce greener alternatives such biofuels. This integrated approach provides an ambitious strategy address global concerns pertaining economic stability, environmental degradation, crisis. study investigates intricate defense mechanisms deployed freshwater microalgae Chlorella minutissima response Cr (III) toxicity. microalga achieved impressive 92% removal efficiency with IC50 value 200 ppm, illustrating its extraordinary resilience towards chromium-induced stress. Furthermore, this research embarked on thorough explorations encompassing morphological, pigment-centric, biochemical analyses, aimed at revealing adaptive strategies associated resilience, well dynamics carbon pool flow that contribute enhanced lipid extracellular polysaccharide (EPS) synthesis. FAME profile biodiesel produced complies benchmark established American European regulations, emphasizing suitability high-quality vehicular fuel. Elevated levels ROS, TBARS, osmolytes (such glycine-betaine), along increased activity antioxidant enzymes (CAT, GR, SOD), reveal activation robust against oxidative stress caused (III). finding investigation presents effective framework algal-based biorefinery approach, integrating pollutant detoxification generation vehicular-quality additional value-added compounds vital under concept circular economy.

Language: Английский

Amphora coffeiformis extracellular polymeric substances and their potential applications in lead removal DOI
Jihen Elleuch, Marwa Drira,

Imtinen Ghribi

et al.

Antonie van Leeuwenhoek, Journal Year: 2025, Volume and Issue: 118(3)

Published: Feb. 3, 2025

Language: Английский

Citations

1
Molecular and Enzymatic Responses of Chlorococcum dorsiventrale to Heavy Metal Exposure: Implications for Their Removal DOI Creative Commons

Rihab Hmani,

Jihen Elleuch, Fatma Elleuch

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(18), P. 8551 - 8551

Published: Sept. 23, 2024

Heavy metals are one of the main threats to marine life and ecosystems any remedial action in that regard is urgently required. The aim this work study bioremoval cadmium, chromium lead a microalgae strain Chlorococcum dorsiventrale isolated from Tunisian coastal waters along with assessing its enzymatic molecular responses. were tested artificial seawater evaluate their capacity for phycoremediation an aquatic environment. This tolerated exposure Cd (II), Cr (VI), Pb (II) was able grow 14 days. exposures elicited decrease chlorophyll, lipid polysaccharide contents, whereas no damages detected following treatment. For protein content, significant changes seen except after which induced slight increase treatment 5 mg/L. assessment stress defense-related gene expression using qRT-PCR revealed up-regulation catalase, superoxide dismutase photosystem II D1 encoding genes. Moreover, heat shock 70 slightly overexpressed. Removal efficiencies attained 89% 95%, respectively. mechanisms by C. removed involved both intracellular extracellular biosorption, while predominantly through membrane adsorption. highlights potential as efficient agent bioremediation heavy metal-contaminated water, including industrial wastewater, thus paving way practical environmental applications pollution control.

Language: Английский

Citations

4
Biotechnological Potential of Newly Isolated Microalga Strain in Cu and Cr Biosorption from Single and Bimetallic Systems DOI Open Access

Imtinen Ghribi,

Jihen Elleuch, Pascal Dubessay

et al.

Water, Journal Year: 2025, Volume and Issue: 17(7), P. 999 - 999

Published: March 28, 2025

The contamination of water by heavy metals is among the main ecological challenges society due to industrialization and urbanization. To overcome this issue, various treatment processes have been developed. Phycoremediation considered a promising strategy offering advantages in terms cost-effectiveness. present work aims investigate cellular responses an isolated green microalga strain (Chlamydomonas sp.) chromium (Cr) copper (Cu) exposure single bimetallic systems. At ½ IC50 concentration, metal removal efficiencies were reported: up 58.11 ± 0.979% for Cu 41.4 0.870% Cr When both combined, efficiency improved 57.71 0.832%, whereas showed minimal variation, reaching 58.43 1.059%. Furthermore, appeared negative effect on cell growth photosynthetic pigment accumulation. An enhancement lipid content microalgae cells after and/or exposure, particularly C14:0, C16:0, C20:0, C18:0, C16:1, C18:1, C20:1, as well polysaccharides, was detected, protein decreased. FTIR analysis that several functional groups could be involved phycoremediation process.

Language: Английский

Citations

0
Toxicological Effects of Silver-Modified Bentonite Nanocomposites on Microalgae: Impact on Cell Growth, Antioxidant Enzymes, and Gene Expression DOI Creative Commons

Oumayma Ghariani,

Jihen Elleuch, Anna Maria Ferretti

et al.

Nanomaterials, Journal Year: 2025, Volume and Issue: 15(8), P. 629 - 629

Published: April 20, 2025

The increasing use of nanostructured silver-containing inorganic materials raises concerns about their impact on aquatic organisms. This study assessed the toxicity silver-modified bentonite composites Chlamydomonas sp. Two were tested: silver-exchanged (Ben-Ag) and its reduced form (Ben-Ag (H2)).Microalgae exposed to 0.5 IC50, 1.5 2 IC50. Ben-Ag showed higher than (H2), which even promoted algal growth at low doses. Fluorescence microscopy revealed morphological shrinkage in treated cells. Increased phenol content, elevated malondialdehyde (MDA) levels, altered antioxidant enzyme activities further confirmed toxicity, along with photosynthetic pigments. Transcriptomic analysis significant changes gene expression under exposure. Genes involved photosynthesis (petB, psbL), caspase activity (casp), carotenoid metabolism (Q2CHY) down-regulated, indicating stress-induced damage. In contrast, genes encoding stress response enzymes (SOD, peroxidase), carbon (rbcL, PGQ1), β-carotene biosynthesis (Q2BKT) up-regulated, reflecting cellular defense mechanisms. Overall, highlights high sp., emphasizing importance evaluating environmental risks before using such environments.

Language: Английский

Citations

0
Integrated processes for olive mill wastewater treatment and its revalorization for microalgae culture DOI

Rihab Hachicha,

Jihen Frikha Elleuch, Pascal Dubessay

et al.

International Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 2, 2024

Language: Английский

Citations

0
A Biorefinery Approach Integrating Lipid and EPS Augmentation Along with Cr (III) Mitigation by Chlorella minutissima DOI Creative Commons

Sonia Choudhary,

Mansi Tiwari, Krishna Mohan Poluri

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2047 - 2047

Published: Dec. 11, 2024

The quest for cleaner and sustainable energy sources is crucial, considering the current scenario of a steep rise in consumption fuel crisis, exacerbated by diminishing fossil reserves rising pollutants. In particular, bioaccumulation hazardous substances like trivalent chromium has not only disrupted fragile equilibrium ecological system but also poses significant health hazards to humans. Microalgae emerged as promising solution achieving sustainability due their ability remediate contaminants produce greener alternatives such biofuels. This integrated approach provides an ambitious strategy address global concerns pertaining economic stability, environmental degradation, crisis. study investigates intricate defense mechanisms deployed freshwater microalgae Chlorella minutissima response Cr (III) toxicity. microalga achieved impressive 92% removal efficiency with IC50 value 200 ppm, illustrating its extraordinary resilience towards chromium-induced stress. Furthermore, this research embarked on thorough explorations encompassing morphological, pigment-centric, biochemical analyses, aimed at revealing adaptive strategies associated resilience, well dynamics carbon pool flow that contribute enhanced lipid extracellular polysaccharide (EPS) synthesis. FAME profile biodiesel produced complies benchmark established American European regulations, emphasizing suitability high-quality vehicular fuel. Elevated levels ROS, TBARS, osmolytes (such glycine-betaine), along increased activity antioxidant enzymes (CAT, GR, SOD), reveal activation robust against oxidative stress caused (III). finding investigation presents effective framework algal-based biorefinery approach, integrating pollutant detoxification generation vehicular-quality additional value-added compounds vital under concept circular economy.

Language: Английский

Citations

0