New Insights in Microplastic Cellular Uptake Through a Cell-Based Organotypic Rainbow-Trout (Oncorhynchus mykiss) Intestinal Platform

Cells, Journal Year: 2025, Volume and Issue: 14(1), P. 44 - 44

Published: Jan. 3, 2025

Microplastics (MPs) in fish can cross the intestinal barrier and are often bioaccumulated several tissues, causing adverse effects. While impacts of MPs on well documented, mechanisms their cellular internalization remain unclear. A rainbow-trout (Oncorhynchus mykiss) platform, comprising proximal distal epithelial cells cultured an Alvetex scaffold, was exposed to 50 mg/L (size 1–5 µm) for 2, 4, 6 h. MP uptake faster RTpi-MI compared RTdi-MI. Exposure microplastics compromised integrity by disrupting tight-junction protein zonula occludens-1, inducing significant decreases transepithelial-electrical-resistance (TEER) values. Consequently, were internalized fibroblasts. The expression genes related endocytosis (cltca, cav1), macropinocytosis (rac1), tight junctions’ formation (oclna, cldn3a, ZO-1) analyzed. No differences observed cltca, oclna, cldn3a expression, while upregulation cav1, rac1, ZO-1 detected, suggesting as route internalization, since also cav1 indirectly this mechanism. obtained results consistent with data previously reported vivo, confirming its validity identifying pathways. This could help develop strategies mitigate absorption through ingestion.

Language: Английский

Effect of black soldier fly (Hermetia illucens L.) larvae meal on growth performance, carcass characteristics, meat quality, and cecal microbiota in broiler chickens

Frontiers in Animal Science, Journal Year: 2025, Volume and Issue: 6

Published: March 20, 2025

The current study was conducted to evaluate the effects of Black Soldier Fly (BSF) larvae meal in broiler chicken diets on growth performance, carcass characteristics, meat quality, and cecal microbiota. 300 unsexed Arbor Acres chicks were divided into two treatment groups, each with six replicates 25 chicks. experimental group fed a diet which soybean partially substituted 5% BSF during starter phase (1–14 days) 10% grower (15–28 days). finisher period is BSF-free diet. Results showed no significant difference performance between control except for higher average weight second week (P=0.016). A observed yield hot eviscerated carcasses (P=0.001), while differences found quality parameters, including pH, myoglobin, water-holding capacity, cooking loss, chemical composition (dry matter, ash, ether extract), bacterial counts such as total aerobic mesophilic flora, Enterobacteriaceae E. coli . inclusion reduced harmful bacteria increased beneficial Lactobacillus spp., chicken’s These findings suggest that provide health benefits forms, supporting their potential viable sustainable alternative protein source replace diets.

Language: Английский

Mitigation of Dietary Microplastic Accumulation and Oxidative Stress Response in Rainbow Trout (Oncorhynchus mykiss) Fry Through Dietary Supplementation of a Natural Microencapsulated Antioxidant

Animals, Journal Year: 2025, Volume and Issue: 15(7), P. 1020 - 1020

Published: April 1, 2025

Microplastic (MP) contamination in aquafeed poses a significant risk to fish health and safety. This study evaluated the effectiveness of microencapsulated natural antioxidant, astaxanthin (AX), mitigating adverse effects dietary MPs rainbow trout fry. The microcapsules were composed an organic wall matrix designed preserve AX while limiting MP absorption intestine. During 60-day feeding trial, fed diets containing amino formaldehyde polymer fluorescent microbeads (1-5 µm; 50 mg/kg), either alone or combination with AX. localization tissues was assessed via confocal microscopy, quantification performed following chemical tissue digestion. Fish welfare using histological molecular analyses. No on growth gut morphology observed across experimental groups. However, mainly translocated liver, where they induced oxidative stress, as evidenced by upregulation sod1, sod2, cat gene expression. inclusion significantly mitigated stress response, facilitated coagulation gut, reducing intestinal absorption. These findings highlight potential antioxidants counteract MP-induced reduce bioavailability aquaculture species, contributing improved product quality.

Language: Английский