Exploring the Increased Activity of the Blue Light-Dependent Photoenzyme Fatty Acid Photodecarboxylase under Violet Light

ACS Catalysis, Journal Year: 2025, Volume and Issue: unknown, P. 6088 - 6097

Published: March 31, 2025

Fatty acid photodecarboxylase (FAP) is a FAD-containing enzyme that catalyzes the light-driven decarboxylation of medium-long chain fatty acids. Over recent years, substrate scope FAP has been broadened to improve its potential catalyze range blue light-dependent industrially relevant reactions. However, photoinactivation constitutes major hurdle for generalized applications. Previous studies have suggested violet light may be more suitable illumination wavelength many these Here, we investigated any possible enhancement in catalytic activity upon with and utilized spectrophotometric assay detects production CO2 real time monitor reaction under different conditions. We show at low intensities approximately 6-fold higher than identical conditions light. Moreover, product yield increases further when delivered pulsed manner, most likely as result lower levels observed continuous illumination. More detailed measurements confirmed employs similar cycle both Rather, efficiency attributable populations excited state FAD species can proceed along productive pathway. suggest pulses low-intensity provide an optimized route catalysis, highlighting importance expanding field flavin-based photobiocatalysis.

Language: Английский

Bridging chemistry and biology for light-driven new-to-nature enantioselective photoenzymatic catalysis

Chemical Society Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Merging enzymes with light-driven photocatalysis has given rise to the burgeoning field of photoenzymatic catalysis.

Language: Английский