Genomic epidemiology and public health implications of zoonotic monophasic Salmonella Typhimurium ST34

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: March 11, 2025

Background Monophasic Salmonella Typhimurium sequence type 34 (mSTM ST34) has emerged as a significant global health threat, but our understanding of its genomic epidemiology and potential public implications in international regional contexts remains limited. This study aims to fill this crucial gap by assessing the multidrug resistance (MDR) mSTM ST34, well clinical characteristics virulence. Methods To achieve objectives study, we conducted comprehensive analysis ST34 isolates. We obtained dataset comprising 13,844 strains from databases, along with 339 surveillance collection Zhejiang Province, China. provide in-depth insights into antimicrobial resistance, mobile genetic elements, pathogenicity. Additionally, meticulously assessed association between phenotypic profiles presentations. Results Our findings revealed that prevalence surpassed previously dominant ST19. In addition, observed an increase detection IncQ1 plasmid, which is responsible for disseminating MDR. The carriage was exceptionally high among children (≤12 years old) elderly individuals (≥65 old), 92.6% isolates exhibiting MDR, including frontline antimicrobials such third-generation cephalosporins ciprofloxacin. human strain demonstrates remarkable capacity biofilm formation, increases virulence animal models complicates therapeutic interventions. Conclusions ST19, ability transmit across multi-species further transmission. addresses critical gaps prevalence, highlighting importance whole genome sequencing surveilling zoonotic pathogens.

Language: Английский

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The Microbiome in Asthma Heterogeneity: The Role of Multi‐Omic Investigations

Immunological Reviews, Journal Year: 2025, Volume and Issue: 330(1)

Published: March 1, 2025

ABSTRACT Asthma is one of the most prevalent and extensively studied chronic respiratory conditions, yet heterogeneity asthma remains biologically puzzling. Established factors like exogenous exposures treatment adherence contribute to variability in risk clinical outcomes. It also clear that endogenous genetics immune system response patterns play key roles asthma. Despite significant existing knowledge above, divergent trajectories outcomes are still observed, even among individuals with similar profiles, biomarkers, optimal medical management. This suggests uncaptured biological interactions asthma's heterogeneity, for which role host microbiota has lately attracted much research attention. review will highlight recent evidence this area, focusing on bedside‐to‐bench investigations have leveraged omic technologies uncover microbiome links immunobiology. Studies centered use multi‐omics noted particular. These represent a new generation reverse‐translational revealing potential functional crosstalk microbiomes may drive phenotypic diseases Multi‐omic data offer wide lens into ecosystem within host. informs hypotheses experimental work elucidate mechanistic pathways unresolved endotypes. Further incorporation patient‐centered can yield insights hopefully lead more precise, microbiome‐informed strategies reduce burden.

Language: Английский

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Toxicity mechanism analysis of cGAS-STING-TBK1 signaling pathway small molecule modulator based on network toxicology and molecular docking strategy: quinacrine acetate as an example

Frontiers in Chemistry, Journal Year: 2025, Volume and Issue: 13

Published: April 22, 2025

Objective This study aims to investigate the toxicity characteristics and mechanisms of quinacrine acetate, a small molecule modulator cGAS-STING-TBK1 signaling pathway, establish validate application value network toxicology analysis strategy. Methods ProTox ADMETlab platforms were used evaluate toxic effects acetate on human tissues organs. Potential targets associated with identified through ChEMBL, STITCH, GeneCards, OMIM, TD databases. GO KEGG analyses employed elucidate related functions molecular mechanisms. STRING Cytoscape software utilized identify key hub genes, while docking validation was performed using CB-Dock2 database. Based results, COPD selected as disease model, GEO database analyze expression characteristics, immune correlation, drug target genes in COPD. Results revealed that exhibited significant respiratory system (toxicity level 4, risk coefficient 0.959). Through integrated multi-database analysis, 14 potential acetate-induced identified. pathway indicated primarily mediated metabolic pathways. Network via AKT1, PLA2G4A, ALOX5 three core targets. Molecular results confirmed strong binding affinity between these In patients, PLA2G4A showed significantly upregulated expression, gene ROC curve AUC reaching 0.829, demonstrating good diagnostic value. Further correlation closely various cell expressions served for multiple drugs including histamine, melittin, formic acid. Conclusion demonstrates may influence progression diseases by regulating The findings provide not only theoretical foundation understanding but also new perspectives methodological references evaluating compounds diseases. Therefore, we practical an efficient predictive tool identifying pathways, which can guide subsequent experimental mechanistic insights traditional approaches might miss.

Language: Английский

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Targeting Calprotectin S100A8/A9 to Overcome AML Progression in DNMT3A-Mutant Cells

Current Medical Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Language: Английский

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The Respiratory Tract Microbiome and Human Health

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(5)

Published: April 28, 2025

ABSTRACT The respiratory tract microbiome (RTM) is a multi‐kingdom microbial ecosystem that inhabits various niches of the system. While previously overlooked, there now sufficient evidence RTM plays crucial role in human health related to immune system training and protection against pathogens. Accordingly, dysbiosis or disequilibrium has been linked several communicable non‐communicable diseases, highlighting need unveil its disease. Here, we define place medicine. Moreover, outline challenges research, emphasising for combining methodologies, including multi‐omics computational tools. We also discuss RTM's potential diagnosing, preventing treating diseases developing novel microbiome‐based therapies improve pulmonary health.

Language: Английский

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The intricate interplay among microbiota, mucosal immunity, and viral infection in the respiratory tract

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 29, 2025

The mucosal system serves as the primary barrier against respiratory diseases and plays a crucial role in combating viral infections through immunity. resident microbial community constitutes main component of exerts significant inhibitory impact on invasion exogenous agents. However, precise relationship between microbiota, immunity, remains incomplete. This review aims to summarize regulatory interactions microbiota innate immune components such immunity trained By clarifying these complex relationships, this seeks identify potential targets for augmenting disease prevention strategies developing novel vaccine formulations. Furthermore, we propose possibility integrating fields microbiome-based therapeutics development create multifunctional formulations capable targeting induction. Such an approach holds great offering pathways treatment diseases.

Language: Английский

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Harnessing human microbiomes for disease prediction

Trends in Microbiology, Journal Year: 2024, Volume and Issue: 32(7), P. 707 - 719

Published: Jan. 21, 2024

Language: Английский

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Environmental exposures, the oral–lung axis and respiratory health: The airway microbiome goes on stage for the personalized management of human lung function

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(6)

Published: June 1, 2024

The human respiratory system is constantly exposed to environmental stimuli, sometimes including toxicants, which can trigger dysregulated lung immune responses that lead symptoms, impaired function and airway diseases. Evidence supports the microbiome in lungs has an indispensable role health disease, acting as a local gatekeeper mediates interaction between cues health. Moreover, intimately intertwined with oral through oral-lung axis. Here, we discuss intricate three-way relationship (i) cigarette smoking, strong effects on microbial community structure of lung; (ii) dysbiosis disease cavity; (iii) its causal patients suffering chronic obstructive pulmonary (COPD), leading cause morbidity mortality worldwide. We highlight exciting outcomes arising from recently established interactions exposures, microbiome, metabolites-functional attributes host, well how these associations have potential predict status host index. For completion, argue incorporating (synthetic) ecology our contemporary understanding presents challenges also rises novel opportunities exploit axis towards innovative diagnostics, prognostics, patient stratification microbiota-targeted clinical interventions context current therapies.

Language: Английский

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The relevance of eosinophils in chronic obstructive pulmonary disease: inflammation, microbiome, and clinical outcomes

Journal of Leukocyte Biology, Journal Year: 2024, Volume and Issue: 116(5), P. 927 - 946

Published: June 28, 2024

Abstract Chronic obstructive pulmonary disease is caused by the inhalation of noxious particles such as cigarette smoke. The pathophysiological features include airway inflammation, alveolar destruction, and poorly reversible airflow obstruction. A subgroup patients with chronic has higher blood eosinophil counts, associated an increased response to inhaled corticosteroids biomarkers type 2 inflammation. Emerging evidence shows that counts have altered microbiome. Higher are also lung function decline, implicating inflammation in progressive pathophysiology disease. We provide a narrative review role eosinophils disease, encompassing microbiome, pharmacological targeting pathways clinical use count biomarker.

Language: Английский

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Call to Action: How Can We Promote the Development of New Pharmacologic Treatments in COPD?

American Journal of Respiratory and Critical Care Medicine, Journal Year: 2024, Volume and Issue: 210(11), P. 1300 - 1307

Published: Oct. 15, 2024

Language: Английский

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