ProQ-associated small RNAs control motility in Vibrio cholerae DOI Creative Commons
Rabea Ghandour,

Daniel Devlitsarov,

Phillip Popp

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

Abstract Gene regulation at the post-transcriptional level is prevalent in all domains of life. In bacteria, ProQ-like proteins have emerged as important RNA chaperones facilitating stability and duplex formation. major human pathogen Vibrio cholerae, gene key for virulence, biofilm formation, antibiotic resistance, yet role ProQ has not been studied. Here, we show that interacts with hundreds transcripts V. including highly abundant FlaX small (sRNA). Global analyses formation using RIL-Seq (RNA interaction by ligation sequencing) revealed a vast network ProQ-assisted interactions identified motility regulation. Specifically, base-pairs multiple sites on flaB flagellin mRNA, preventing 30S ribosome binding translation initiation. cholerae cells lacking flaX display impaired expression, altered flagella composition reduced swimming liquid environments. Our results provide global view ProQ-associated pinpoint mechanistic phenotypic consequences associated sRNAs cholerae.

Language: Английский

RNA interactome of hypervirulent Klebsiella pneumoniae reveals a small RNA inhibitor of capsular mucoviscosity and virulence DOI Creative Commons
Kejing Wu, Xingyu Lin,

Yujie Lu

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 13, 2024

Hypervirulent Klebsiella pneumoniae (HvKP) is an emerging bacterial pathogen causing invasive infection in immune-competent humans. The hypervirulence strongly linked to the overproduction of hypermucoviscous capsule, but underlying regulatory mechanisms hypermucoviscosity (HMV) have been elusive, especially at post-transcriptional level mediated by small noncoding RNAs (sRNAs). Using a recently developed RNA interactome profiling approach iRIL-seq, we interrogate Hfq-associated sRNA network and establish intracellular RNA-RNA HvKP. Our data reveal numerous interactions between sRNAs HMV-related mRNAs, identify plethora that repress or promote HMV. One strongest HMV repressors ArcZ, which activated catabolite regulator CRP targets many genes including mlaA fbp. We discover MlaA its function phospholipid transport crucial for capsule retention HMV, inactivation abolishes virulence mice. ArcZ overexpression drastically reduces burden mice multiple hypervirulent carbapenem-resistant clinical isolates, indicating potent inhibitor pneumonia with therapeutic potential. work unravels novel CRP-ArcZ-MlaA circuit provides mechanistic insights into posttranscriptional control superbug global concern. By performing analysis pneumoniae, authors key factor MlaA, inhibiting pathogenesis

Language: Английский

Citations

7
Invasive bacteriophages between a bell and a hammer: a comprehensive review of pharmacokinetics and bacterial defense systems DOI Creative Commons

Mohammed F Hamdi,

Alyaa Abdulhussein Alsaedi,

Alaa Qasim Hayder

et al.

Discover Life, Journal Year: 2025, Volume and Issue: 55(1)

Published: April 3, 2025

Language: Английский

Citations

0
Non-genetic messenger RNA silencing reveals essential genes in phage-host interplay DOI Creative Commons
Milan Gerovac, Svetlana Đurica-Mitić,

Leandro Buhlmann

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 31, 2024

Bacteriophages are the most abundant entities on earth and exhibit vast genetic phenotypic diversity. Exploitation of this largely unexplored molecular space requires identification functional characterisation genes that act at phage-host interface. To date, is restricted to few model systems amenable manipulation. overcome limitation, we introduce a non-genetic mRNA targeting approach using exogenous delivery programmable antisense oligomers (ASOs) silence both DNA RNA phages. A systematic knockdown screen core accessory nucleus-forming jumbo phage ΦKZ, coupled RNA-sequencing microscopy analyses, reveals previously unrecognised proteins essential for replication that, upon silencing, elicit distinct phenotypes level host response. One these factors ΦKZ-encoded nuclease acts major decision point during infection, linking formation protective nucleus genome amplification. The ASO-based gene silencing used here promises be versatile tool discovery in biology, will help elucidate defence anti-defence mechanisms non-model pairs, offers potential optimising therapy biotechnological procedures.

Language: Английский

Citations

3
A review on the crosstalk between non-coding RNAs and the cGAS-STING signaling pathway DOI
Zijian Xiong, Yu Wang, Zhaoqi Li

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 283, P. 137748 - 137748

Published: Nov. 19, 2024

Language: Английский

Citations

1
Slings and arrows: sRNAs mediate intragenomic competition DOI Creative Commons
Micah J. Ferrell, Christopher M. Waters

Cell Host & Microbe, Journal Year: 2024, Volume and Issue: 32(5), P. 634 - 636

Published: May 1, 2024

Language: Английский

Citations

0
Phage-encoded small RNA hijacks host replication machinery to support the phage lytic cycle DOI Creative Commons

Aviezer Silverman,

Raneem Nashef,

Robert Bruner

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 31, 2024

ABSTRACT Bacteriophages (phages) significantly influence bacterial populations in their natural environment. However, one aspect that has not been thoroughly explored the context of phage-bacteria interactions is post-transcriptional regulation gene expression, despite growing recognition its importance physiology over past two decades. Important players this process are small RNAs (sRNAs) regulate target mRNAs via base-pairing, typically using RNA chaperones like Hfq to facilitate regulation. Here, we apply RIL-seq map in-vivo sRNA-RNA network Escherichia coli upon lambda phage infection. We highlight changes transcriptome and sRNA interactome while uncovering a novel phage-encoded regulates key genes E. . decipher molecular mechanism sRNA-mediated illustrate how hijacks host replication machinery helps infection cycle. Overall, uncover an RNA-level regulatory layer shapes - interactions.

Language: Английский

Citations

0
Biological Insights from RNA–RNA Interactomes in Bacteria, as Revealed by RIL-seq DOI

Aviezer Silverman,

Sahar Melamed

Methods in molecular biology, Journal Year: 2024, Volume and Issue: unknown, P. 189 - 206

Published: Nov. 15, 2024

Language: Английский

Citations

0
ProQ-associated small RNAs control motility in Vibrio cholerae DOI Creative Commons
Rabea Ghandour,

Daniel Devlitsarov,

Phillip Popp

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

Abstract Gene regulation at the post-transcriptional level is prevalent in all domains of life. In bacteria, ProQ-like proteins have emerged as important RNA chaperones facilitating stability and duplex formation. major human pathogen Vibrio cholerae, gene key for virulence, biofilm formation, antibiotic resistance, yet role ProQ has not been studied. Here, we show that interacts with hundreds transcripts V. including highly abundant FlaX small (sRNA). Global analyses formation using RIL-Seq (RNA interaction by ligation sequencing) revealed a vast network ProQ-assisted interactions identified motility regulation. Specifically, base-pairs multiple sites on flaB flagellin mRNA, preventing 30S ribosome binding translation initiation. cholerae cells lacking flaX display impaired expression, altered flagella composition reduced swimming liquid environments. Our results provide global view ProQ-associated pinpoint mechanistic phenotypic consequences associated sRNAs cholerae.

Language: Английский

Citations

0