Targeted degradation of endogenous condensation-prone proteins improves crop performance DOI Creative Commons
Ming Luo, Qing Wen, Sitao Zhu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Abstract Effective methods, such as CRISPR and RNA interference, exist for modulating gene expression at DNA levels, but approaches directly modifying endogenous proteins remain lacking in plants. Here, we develop a targeted condensation-prone-protein degradation (TCD) strategy to eliminate proteins, particularly those prone condensation. We identify an E3 ligase, E3TCD1, that degrades itself selectively targets other when fused them. In rice, transgenic E3TCD1 fusions with Teosinte branched 1 Early flowering 3 (OsELF3) modulate tiller numbers times, respectively. The TCD system is also controllable. Using the Pro TBF1 -uORFs control cassette, can conditionally degrade negative defense regulator OsELF3 upon pathogen invasion, enhancing rice resistance without interfering time. This method, unlike animal-targeting strategies, does not rely on small molecules, antibodies, or genetic knock-ins, showing promise therapeutic avenue optimizing crop performance potentially addressing human diseases.

Language: Английский

Engineering crop performance with upstream open reading frames DOI

Rui Mou,

Ruixia Niu,

Rong‐Cai Yang

et al.

Trends in Plant Science, Journal Year: 2024, Volume and Issue: 30(3), P. 311 - 323

Published: Oct. 30, 2024

Language: Английский

Citations

4
Genetically-encoded targeted protein degradation technology to remove endogenous condensation-prone proteins and improve crop performance DOI Creative Commons
Ming Luo, Sitao Zhu,

Hua Dang

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 29, 2025

Effective modulation of gene expression in plants is achievable through tools like CRISPR and RNA interference, yet methods for directly modifying endogenous proteins remain lacking. Here, we identify the E3 ubiquitin ligase E3TCD1 develope a Targeted Condensation-prone-protein Degradation (TCD) strategy. The X-E3TCD1 fusion protein acts as genetically engineered degrader, selectively targeting prone to condensation. For example, transgenic with Teosinte branched 1 (TB1) degrades native TB1 protein, resulting increased tiller numbers rice. Additionally, conditional degradation negative defense regulator Early Flowering 3 via pathogen-responsive ProTBF1-uORFsTBF1 cassette enhances rice blast resistance without affecting flowering time absence pathogen. Unlike prevailing targeted strategies, TCD system does not rely on small molecules, antibodies, or genetic knock-in tags, demonstrating its promise transgene-based approach optimizing crop performance.

Language: Английский

Citations

0
Molecular condensates as roadblocks in plant defense DOI
Jianwei Li, Yan Liang

Cell Host & Microbe, Journal Year: 2024, Volume and Issue: 32(9), P. 1461 - 1463

Published: Sept. 1, 2024

Language: Английский

Citations

0
Targeted degradation of endogenous condensation-prone proteins improves crop performance DOI Creative Commons
Ming Luo, Qing Wen, Sitao Zhu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Abstract Effective methods, such as CRISPR and RNA interference, exist for modulating gene expression at DNA levels, but approaches directly modifying endogenous proteins remain lacking in plants. Here, we develop a targeted condensation-prone-protein degradation (TCD) strategy to eliminate proteins, particularly those prone condensation. We identify an E3 ligase, E3TCD1, that degrades itself selectively targets other when fused them. In rice, transgenic E3TCD1 fusions with Teosinte branched 1 Early flowering 3 (OsELF3) modulate tiller numbers times, respectively. The TCD system is also controllable. Using the Pro TBF1 -uORFs control cassette, can conditionally degrade negative defense regulator OsELF3 upon pathogen invasion, enhancing rice resistance without interfering time. This method, unlike animal-targeting strategies, does not rely on small molecules, antibodies, or genetic knock-ins, showing promise therapeutic avenue optimizing crop performance potentially addressing human diseases.

Language: Английский

Citations

0