Metabolic Regulation and Cardiorenal Protection: Deciphering the Potential and Multi-Target Mechanisms of Jiangtang Qingre Formula for Type 2 Diabetes Management

Published: Jan. 1, 2025

Language: Английский

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Absolute Quantitative Metagenomic Analysis Provides More Accurate Insights for the Anti-Colitis Effect of Berberine via Modulation of Gut Microbiota

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 400 - 400

Published: March 11, 2025

Current gut microbiota studies often rely on relative quantitative sequencing. However, under certain circumstances, while the abundance of these bacteria may remain stable, absolute quantities specific can vary considerably. Since function is directly linked to their total numbers, quantification crucial. This study aims identify optimal method for microbiome analysis by comparing and Using ulcerative colitis, which closely associated with microbiota, as a disease model berberine (which affects microbiota) versus sodium butyrate does not) drugs, methods were used evaluate varying effects different drugs regulation in UC-affected animals. The regulatory BBR further synthesized identified earlier using an individual-based meta-analysis, we compared findings our sequencing results. results from more consistent actual microbial community, suggesting that measurements might not accurately reflect true species. Moreover, meta-analysis only partially sometimes opposed, analyses are prone misinterpretation incorrect correlation underscores importance representing counts sample evaluating modulatory microbiome, plays vital role microbiome.

Language: Английский

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Dangua Fang induces anti-glucolipid metabolism disorder effects similar to those of direct NFIL3 inhibition

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 19, 2025

Background Dangua Fang (DGF) is a traditional Chinese herbal formula widely used to regulate glucolipid metabolism. Nuclear factor, interleukin-3 regulated (NFIL3) plays regulatory role in intestinal fat absorption and energy Gut microbiota can modulate NFIL3 expression affect host Purpose This study aimed investigate the effects of DGF or inhibition on gut their metabolites mice with metabolism disorder (GLMD) explore relationship between anti-GLMD those direct inhibition. Methods A GLMD mouse model was established by induction high-glucose high-fat diet. The were divided into control group (CG), (MG), (DFG), + siRNA (DFSG), (SG). administered sterile water, DGF, and/or intraperitoneal injections siRNA-NFIL3 normal saline for 15 weeks, following which metabolic indicators, levels, histopathological alterations liver small tissues evaluated. Additionally, differential analysed, linear regression analysis conducted microbial species indicators assess regulation. Results Significant differences observed CG MG groups various indicators. Compared that group, DFG, DFSG, SG significantly improved, pathological morphology intestine altered. mRNA protein levels serum, liver, decreased. relative abundance Bacteroidota decreased, whereas Firmicutes increased, changes correlated serum total cholesterol (TC), triglyceride (TG), free fatty acid (FFA) levels. Moreover, lipid metabolism-related pathways altered all three intervention groups. Conclusion reduced mice, metabolites, pathways, similar

Language: Английский

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Electroacupuncture modulates the gut-brain axis via the PI3K/Akt pathway to improve feeding behavior, body weight, glucolipid metabolism, and reduce insulin resistance in T2DM rats

Diabetology & Metabolic Syndrome, Journal Year: 2025, Volume and Issue: 17(1)

Published: April 9, 2025

Language: Английский

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Gut microbiome research: Revealing the pathological mechanisms and treatment strategies of type 2 diabetes

Diabetes Obesity and Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Abstract The high prevalence and disability rate of type 2 diabetes (T2D) caused a huge social burden to the world. Currently, new mechanisms therapeutic approaches that may affect this disease are being sought. With in‐depth research on pathogenesis T2D growing advances in microbiome sequencing technology, association between gut microbiota has been confirmed. participates regulation inflammation, intestinal permeability, short‐chain fatty acid metabolism, branched‐chain amino metabolism bile thereby affecting host glucose lipid metabolism. Interventions focusing gaining traction as promising approach management. For example, dietary intervention, prebiotics probiotics, faecal transplant phage therapy. Meticulous experimental design choice analytical methods crucial for obtaining accurate meaningful results from studies. How choose different machine learning data analysis extremely critical achieve personalized precision medicine.

Language: Английский

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Gut Microbiota Metabolites and Chronic Diseases: Interactions, Mechanisms, and Therapeutic Strategies

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3752 - 3752

Published: April 16, 2025

The gut microbiota, shaped by factors such as diet, lifestyle, and genetics, plays a pivotal role in regulating host metabolism, immune function, overall health. diversity balance of the microbiota are closely linked to onset progression various chronic diseases. A growing body evidence has demonstrated that alterations composition, metabolites significantly associated with cardiovascular diseases, including hypertension, atherosclerosis, heart failure; metabolic disorders obesity, type 2 diabetes, dysfunction-associated steatotic liver disease; gastrointestinal conditions like inflammatory bowel disease colorectal cancer. Despite substantial advances microbiome research, challenges remain fully elucidating causal relationships between disease, well translating these insights into clinical applications. This review aims investigate regulatory pathways via which affects health, disease. Additionally, it highlights emerging strategies for prevention treatment conditions, focusing on microbiota-targeted therapies personalized dietary interventions promising approaches improving health outcomes.

Language: Английский

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Uncovering the heterogeneity of the gut microbial taxa associated with the contents of different fatty acids in muscle with cecum luminal content and fecal samples from two pig populations

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 30, 2025

Fatty acids in pork are involved cellular physiological functions and related to meat nutrition, tenderness, flavor. Increasing evidences have suggested that short-chain fatty produced by the gut microbiota may affect host metabolism energy utilization. However, association between long-chain (LCFAs) has been largely unknown. In this study, microbial compositions of 243 cecum content samples from Erhualian pigs 235 fecal Bamaxiang were determined high throughput 16S rRNA gene sequencing. The contents 12 LCFAs longissimus dorsi (LD) muscle also for all experimental both pig populations. We systematically evaluated contribution variations acid α -diversity microbiota, co-abundance groups (CAGs) Amplicon Sequence Variants (ASVs), acid-associated bacterial taxa. identified hundred ASVs > 40 taxa significantly associated with two Different numbers specific detected luminal samples, suggesting heterogeneity LCFA-associated locations. uncovered some interesting associations contents. strongest was observed ASV annotated Akkermansia n-6/n-3 polyunsaturated ratio ( p = 6.45E-04, Z −9.65). could explain 1.47–4.62% variation twelve acids. functional prediction analysis KEGG pathways metabolisms carbohydrate lipids, fat digestion absorption positively adipocytokine signaling pathway thermogenesis negatively results study provided basic knowledge improving regulating microbiome.

Language: Английский

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Gut Commensal Barnesiella Intestinihominis Ameliorates Hyperglycemia and Liver Metabolic Disorders

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Abstract Recent studies have highlighted the role of gut microbiota in type 2 diabetes (T2D). Improving dysbiosis can be a potential strategy for prevention and management T2D. Here, this work finds that abundance Barnesiella intestinihominis is significantly decreased fecal T2D patients from 2‐independent centers. Oral treatment live B. (LBI) considerably ameliorates hyperglycemia liver metabolic disorders HFD/STZ‐induced models db/db mice. LBI‐derived acetate has similar protective effects against Mechanistically, enhances fibroblast growth factor 21 (FGF21) through inhibition histone deacetylase 9 (HDAC9) to increase H3K27 acetylation at FGF21 promoter. The screening puerarin Gegen Qinlian decoction microbiota‐dependent manner improved by promoting . This study suggests commensal puerarin, respectively as probiotic prebiotic

Language: Английский

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High-fat diet promotes type 2 diabetes mellitus by disrupting gut microbial rhythms and short-chain fatty acid synthesis

Food & Function, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

A schematic diagram of a high-fat diet promotes type 2 diabetes mellitus by disrupting gut microbial rhythms and short-chain fatty acid synthesis.

Language: Английский

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Unlocking the secrets of the human gut microbiota: Comprehensive review on its role in different diseases

World Journal of Gastroenterology, Journal Year: 2024, Volume and Issue: 31(5)

Published: Dec. 30, 2024

The human gut microbiota, a complex and diverse community of microorganisms, plays crucial role in maintaining overall health by influencing various physiological processes, including digestion, immune function, disease susceptibility. balance between beneficial harmful bacteria is essential for health, with dysbiosis - disruption this linked to numerous conditions such as metabolic disorders, autoimmune diseases, cancers. This review highlights key genera Enterococcus, Ruminococcus, Bacteroides, Bifidobacterium, Escherichia coli, Akkermansia muciniphila, Firmicutes (including Clostridium Lactobacillus), Roseburia due their well-established roles regulation but other bacteria, Clostridioides difficile, Salmonella, Helicobacter pylori, Fusobacterium nucleatum, are also implicated diseases. Pathogenic coli Bacteroides fragilis, contribute inflammation cancer progression disrupting responses damaging tissues. potential microbiota-based therapies, probiotics, prebiotics, fecal microbiota transplantation, dietary interventions, improve outcomes examined. Future research directions the integration multi-omics, impact diet lifestyle on composition, advancing engineering techniques discussed. Understanding microbiota's formulating personalized, efficacious treatments preventive strategies, thereby enhancing progressing microbiome research.

Language: Английский

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