Transcriptomics and microbiome insights reveal the protective mechanism of mulberry-derived postbiotics against inflammation in LPS-induced mice

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 18, 2025

Natural food-derived bioactive compounds have garnered increasing attention for their potential to modulate immune responses and promote gut health. In particular, like mulberry-derived postbiotics (MDP) may offer novel therapeutic strategies address inflammation, a key driver of many metabolic disorders. This study examines the protective effects MDP against inflammation in LPS-induced mice, using transcriptomic microbiome analyses explore underlying mechanisms. pretreatment alleviates LPSinduced villous atrophy intestinal barrier damage, promoting recovery morphology. Transcriptomic profiling revealed significant changes gene expression, with 983 upregulated 1220 downregulated genes NC vs LPS comparison, 380 204 LPS+MDP comparison. Enrichment analysis GO KEGG pathways associations transcriptional regulatory activity, NOD-like receptor signaling pathway among differentially expressed genes. Protein-protein interaction identified involved regulation, hub IL6, CXCL10, MYD88 group CD74, CIITA, H2-AB1 MDP-treated group. Microbiome suggested also influence microbiota composition, supporting systemic regulation. These findings highlight MDP's as food additive modulation

Language: Английский

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Nano-engineered probiotic treats atherosclerosis via inhibiting intestinal microbiota-TMA-TMAO axis

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Considerable numbers of patient are suffering from atherosclerosis without typical risk factors, which can cause severe cardiovascular complication but is lack practical treatment. Thereinto, trimethylamine N-oxide (TMAO), originated enteric microorganism, emerges as an unconventional and crucial factor causing atherosclerosis. Here we demonstrate a strategy to inhibit TMAO through intestinal microbiota-trimethylamine (TMA)-TMAO axis for atherosclerotic The therapy performed by oral-treated nano-engineered probiotic PDMF@LGG, where the Lacticaseibacillus rhamnosus GG (LGG) armed with polydopamine coating conjugated PMF nanoparticles based on ROS-responsive polymeric prodrug fluoromethylcholine (FMC). PDMF@LGG durably colonize canal due sticky protection against ROS-induced injury. ROS trigger delivery FMC nanoparticles, TMA production in microorganisms. Meanwhile, LGG strengthen tight junctions epithelium reduce entering blood. in vivo study suggests that reduces plasma suppresses progression. Furthermore, microbiomics metabolomics show also regulates gut microbial composition various metabolites, assisting therapeutic outcome. Together, offers potential candidate caused broadens range treatable

Language: Английский

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Oral Akkermansia muciniphila Biomimetic Nanotherapeutics for Ulcerative Colitis Targeted Treatment by Repairing Intestinal Epithelial Barrier and Restoring Redox Homeostasis

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

The structural disruption of intestinal barrier and excessive reactive oxygen/nitrogen species (RONS) generation are two intertwined factors that drive the occurrence development ulcerative colitis (UC). Synchronously restoring mitigating excess RONS is a promising strategy for UC management, but its treatment outcomes still hindered by low drug accumulation retention in colonic lesions. Inspired intestine colonizing bacterium, we developed mucoadhesive probiotic Akkermansia muciniphila-mimic entinostat-loaded hollow mesopores prussian blue (HMPB) nanotherapeutic (AM@HMPB@E) UC-targeted therapy via repairing scavenging RONS. After oral administration, negatively charged AM@HMPB@E specifically bind to positively inflamed colon lesions electrostatic interactions muciniphila membrane-mediated bioadhesion mechanism. Subsequently, superoxide dismutase (SOD)-, catalase (CAT)-like HMPB eliminated RONS, thereby alleviating RONS-mediated inflammation epithelial damage. Meanwhile, UC-site locally released entinostat could repair damaged inhibiting endothelial cell apoptosis up-regulating expression tight junctions. Both vitro vivo results shown not only exhibited an exceptional site also demonstrated superior therapeutic efficacy compared first-line sulfasalazine, as evidenced longer colon, less rectal bleeding body weight loss. Collectively, our findings highlight clinical application prospects this synchronous treatment, offering paradigm rational design nanomedicine.

Language: Английский

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Selenium-Enriched Lactiplantibacillus plantarum ZZU 8–12 Regulates Intestinal Microbiota and Inhibits Acute Liver Injury

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Language: Английский

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Shengjiang Xiexin Decoction ameliorates DSS-induced ulcerative colitis via activating Wnt/β-Catenin signaling to enhance epithelium renovation and modulating intestinal flora

Phytomedicine, Journal Year: 2025, Volume and Issue: 139, P. 156456 - 156456

Published: Feb. 1, 2025

Language: Английский

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Dental problems and oral microbiome alterations in ulcerative colitis

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 5, 2025

Ulcerative colitis is a chronic disease that has not well-established etiology. The role of microbial dysregulation in its pathogenesis been recently highlighted. Overall, microbiome alterations concern the reduction bacterial abundance and diversity, resulting gut imbalance negatively affecting immunological aspects. There link between ulcerative oral microbiome. changes are found at many levels, from gently dysbiotic composition to presence main periodontal microbes. analysis can be part personalized medicine due fact it potential biomarker. Patients with may manifest dental symptoms/problems, such as periodontitis (strongly related red-complex pathogens— Porphyromonas gingivalis , Tannerella forsythia Treponema denticola bacteria belonging other complexes, Fusobacterium nucleatum Aggregatibacter actinomycetecomitans ), caries, ulcerations, leukoplakia, halitosis, others. Notably, DMFT (Decayed, Missing, Filled Teeth) index higher these patients compared healthy subjects. According some data, lichen planus (which an background) also observed patients. It seems deep understanding association microbiome, immunology, manifestations crucial provide complex treatment point view.

Language: Английский

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Probiotic Delivery for Editing of the Gut Microbiota to Mitigate Colitis and Maintain Hepatic Homeostasis Via Gut–Liver Axis

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Inflammatory bowel disease (IBD) compromises the intestinal barrier and disrupts gut microbiota, impacting liver function via gut-liver axis, which in turn influences microbiota through lipid metabolites exacerbating IBD. This study introduced a probiotic-based treatment using Lactobacillus acidophilus encapsulated tungsten ion-loaded mesoporous polydopamine (LA@WMPDA) to ameliorate colitis balance enterohepatic homeostasis. After oral administration, encapsulation could protect acidophilus, scavenge reactive oxygen/nitrogen species, released ions would inhibit abnormal Enterobacteriaceae growth during colitis, consequently restoring regulating microbiota. Nontargeted metabolomics transcriptomics analyses showed increased short-chain fatty acids indole derivatives, decreased hepatic metabolism. Pathways associated with immune response, cell migration death, response bacterium significant down-regulation colon transcriptome analysis. Thus, this provided pioneered paradigm for IBD highlighted regulation of liver-related metabolic functions axis.

Language: Английский

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Oral Targeted MXenzyme – Armed Probiotic Regulates Intestinal Redox and Microbiota Homeostasis to Synergistically Ameliorate Ulcerative Colitis

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 16, 2025

Abstract Ulcerative colitis (UC) leads to complex alterations in the intestinal microenvironment, with gut microbiota dysbiosis and excessive reactive oxygen species (ROS) being major drivers. Previously, microecological preparations have been used restore homeostasis demonstrated considerable potential; however, efficacy is hindered due hostile environment gastrointestinal tract high ROS levels at lesion sites. Accordingly, a multienzyme mimicking vanadium carbide (V2C) MXenzyme armored colon‐colonizing Akkermansia muciniphila (Akk) probiotic designed synchronously alleviate inflammation regulate microbiota. V2C induced on boron hydroxyl groups coated onto via boronic acid vicinal‐diol‐based click reactions called Akk@V2C. Akk@V2C targets UC lesional sites Akk colonizing capabilities electrostatic interactions. effectively scavenges enhances colonialization mouse model colon tissue biopsies from patients UC. Furthermore, exhibits notable curative effects by alleviating inflammation, reprogramming macrophage polarization, regulating homeostasis, thus promoting short chain fatty production restoring barriers. Moreover, this approach exerts negligible impact bacterial viability has shown potential other probiotics. Collectively, given its flexibility favorable biocompatibility, promising for biomaterial delivery biomedical applications.

Language: Английский

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Multifunctional Double-Loaded Oral Nanoplatform for Computed Tomography Imaging-Guided and Integrated Treatment of Inflammatory Bowel Disease

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Excessive reactive oxygen species, disruption of the epithelial barrier, immune dysregulation, and gut microbiota imbalance are key factors driving onset inflammatory bowel disease (IBD) complicating its treatment. Prompt diagnosis diseases precise delivery therapeutic agents to inflamed intestinal sites offer promising targeted strategies for effectively treating IBD. Here, a barium sulfate-based nanoplatform (BaSO4@PDA@CeO2/DSP, BPCD) synergistic nanozymes drugs was developed. With enhanced colonic retention after oral drug delivery, this enables effective targeting CT imaging guidance address multiple contributing A comprehensive effect achieved through action cerium oxide with optimized Ce3+/Ce4+ ratio sustained release dexamethasone sodium phosphate. Benefiting from superior gastrointestinal stability, is highly in IBD by alleviating oxidative stress, modulating macrophage polarization balance, flora composition, repairing barrier. BPCD inhibits development mechanisms has biocompatibility, emerging as practical alternative traditional therapies.

Language: Английский

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Probiotic Membrane-Modified Nanocomposite Alleviates Inflammation and Microbiota Dysbiosis in Colitis by Scavenging Oxidative Stress and Restoring Immune Homeostasis

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Inflammatory bowel disease (IBD) is a complex chronic intestinal disorder in which excessive oxidative stress, dysregulated immune response, and microbiota dysbiosis contribute to recurrent episodes of inflammation the colonic mucosa. Current clinical treatments focusing solely on resolution often exhibit limited efficacy due inability fundamentally improve pathological microenvironment. Herein, probiotic membrane-modified drug delivery nanocomposite, namely, MPDA@Cur@EM, developed for comprehensive treatment IBD. It contains two components: curcumin-loaded mesoporous polydopamine nanoparticle (MPDA@Cur) as core Escherichia coli Nissle 1917 outer membrane (EM) surface. For MPDA@Cur, microenvironment triggers responsive release curcumin. Importantly, MPDA@Cur can effectively alleviate inflammatory response LPS-activated macrophages through MPDA-mediated ROS scavenging curcumin-induced M2 polarization. In dextran sulfate sodium (DSS)-induced colitis model, EM coating not only allows targeting enrichment orally administered MPDA@Cur@EM inflamed mucosa, but also participates regulation flora. Consequently, efficiently attenuates reaction restores barrier functions, demonstrated by multipronged manner restoring redox balance, remodeling homeostasis, reshaping gut microecology. Collectively, this work provides safe promising codelivery strategy product, antioxidative nanoenzyme, therapeutic management

Language: Английский

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