Strain-specific galactose utilization by commensalE. colimitigatesSalmonellaestablishment in the gut DOI Creative Commons
Christopher Schubert,

Jana Näf,

Lisa Petukhov

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Abstract Salmonella enterica serovar Typhimurium ( S . Tm) is a major cause of gastrointestinal diseases worldwide. To date, options for prevention or curative therapy remain limited. The gut microbiota plays protective role against enteric diseases, particularly in preventing establishment and proliferation Tm. While most research has focused on microbiota-mediated pathogen exclusion during the later, inflammation-dominated stages infection, little known about how members mitigate Tm early colonization. address this gap, we conducted 24 h vivo competitive experiments using different commensal E. coli strains. We observed significant reduction load, which was strain-specific evident with 8178. investigate underlying molecular mechanisms, performed an screen rationally designed library -which includes wide range carbohydrate utilization mutants - both absence presence Our findings revealed that 8178-mediated competition driven by exploitation galactose stage infection. Identifying as key metabolite enhances our mechanistic understanding protection opens new avenues developing microbiota- dietary-based strategies to better control intestinal infections.

Language: Английский

Strain-specific galactose utilization by commensalE. colimitigatesSalmonellaestablishment in the gut DOI Creative Commons
Christopher Schubert,

Jana Näf,

Lisa Petukhov

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Abstract Salmonella enterica serovar Typhimurium ( S . Tm) is a major cause of gastrointestinal diseases worldwide. To date, options for prevention or curative therapy remain limited. The gut microbiota plays protective role against enteric diseases, particularly in preventing establishment and proliferation Tm. While most research has focused on microbiota-mediated pathogen exclusion during the later, inflammation-dominated stages infection, little known about how members mitigate Tm early colonization. address this gap, we conducted 24 h vivo competitive experiments using different commensal E. coli strains. We observed significant reduction load, which was strain-specific evident with 8178. investigate underlying molecular mechanisms, performed an screen rationally designed library -which includes wide range carbohydrate utilization mutants - both absence presence Our findings revealed that 8178-mediated competition driven by exploitation galactose stage infection. Identifying as key metabolite enhances our mechanistic understanding protection opens new avenues developing microbiota- dietary-based strategies to better control intestinal infections.

Language: Английский

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