International Journal of Applied Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 134 - 141
Published: March 7, 2025
Objective: This study aimed to evaluate the biological activity of benzylidene benzohydrazide derivatives against Cancer Stem Cells (CSCs) through in vitro cytotoxicity tests and silico analyses using molecular docking. Methods: Four hydrazone compounds, namely benzo hydrazide (L1), 2-methyl (L2), 2-nitro (L3), 2-bromobenzylidene (L4) were used for studies. The interaction between compounds EGFR protein receptor (PDB ID: 1m17) was investigated AutoDock tools 1.5.7. PASS server predicted activities substances. ADMET assessed ADMETLab 2.0. Meanwhile, cytotoxic test on CSCs evaluated MTT Assay method. Results: results docking analysis L1-L4 provide binding energy values ranging from -6.69 to-7.74 kcal/mol. value is lower than reference Doxorubicin (-4.30 Kcal/mol). with IC50 L1 0.220±0.360 μg/ml, L2 0.034±0.023 L3 0.355±0.276 L4 1.193±1.122 μg/ml 0.220±0.180 μg/ml. These indicate that have potential be inhibitor. Conclusion: (L2) had as a inhibitor vigorous cell lines
Language: Английский