IN SILICO STUDIES AND CYTOTOXICITY ASSAY OF BENZYLIDENE BENZO HYDRAZIDE DERIVATIVES ON CANCER STEM CELL DOI Open Access
Imanuel Gauru,

Yusuf Syahril Alam,

Mardi Santoso

et al.

International Journal of Applied Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 134 - 141

Published: March 7, 2025

Objective: This study aimed to evaluate the biological activity of benzylidene benzohydrazide derivatives against Cancer Stem Cells (CSCs) through in vitro cytotoxicity tests and silico analyses using molecular docking. Methods: Four hydrazone compounds, namely benzo hydrazide (L1), 2-methyl (L2), 2-nitro (L3), 2-bromobenzylidene (L4) were used for studies. The interaction between compounds EGFR protein receptor (PDB ID: 1m17) was investigated AutoDock tools 1.5.7. PASS server predicted activities substances. ADMET assessed ADMETLab 2.0. Meanwhile, cytotoxic test on CSCs evaluated MTT Assay method. Results: results docking analysis L1-L4 provide binding energy values ranging from -6.69 to-7.74 kcal/mol. value is lower than reference Doxorubicin (-4.30 Kcal/mol). with IC50 L1 0.220±0.360 μg/ml, L2 0.034±0.023 L3 0.355±0.276 L4 1.193±1.122 μg/ml 0.220±0.180 μg/ml. These indicate that have potential be inhibitor. Conclusion: (L2) had as a inhibitor vigorous cell lines

Language: Английский

IN SILICO STUDIES AND CYTOTOXICITY ASSAY OF BENZYLIDENE BENZO HYDRAZIDE DERIVATIVES ON CANCER STEM CELL DOI Open Access
Imanuel Gauru,

Yusuf Syahril Alam,

Mardi Santoso

et al.

International Journal of Applied Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 134 - 141

Published: March 7, 2025

Objective: This study aimed to evaluate the biological activity of benzylidene benzohydrazide derivatives against Cancer Stem Cells (CSCs) through in vitro cytotoxicity tests and silico analyses using molecular docking. Methods: Four hydrazone compounds, namely benzo hydrazide (L1), 2-methyl (L2), 2-nitro (L3), 2-bromobenzylidene (L4) were used for studies. The interaction between compounds EGFR protein receptor (PDB ID: 1m17) was investigated AutoDock tools 1.5.7. PASS server predicted activities substances. ADMET assessed ADMETLab 2.0. Meanwhile, cytotoxic test on CSCs evaluated MTT Assay method. Results: results docking analysis L1-L4 provide binding energy values ranging from -6.69 to-7.74 kcal/mol. value is lower than reference Doxorubicin (-4.30 Kcal/mol). with IC50 L1 0.220±0.360 μg/ml, L2 0.034±0.023 L3 0.355±0.276 L4 1.193±1.122 μg/ml 0.220±0.180 μg/ml. These indicate that have potential be inhibitor. Conclusion: (L2) had as a inhibitor vigorous cell lines

Language: Английский

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