Urinary Catheters Coated with a Novel Biofilm Preventative Agent Inhibit Biofilm Development by Diverse Bacterial Uropathogens

Antibiotics, Journal Year: 2022, Volume and Issue: 11(11), P. 1514 - 1514

Published: Oct. 30, 2022

Despite the implementation of stringent guidelines for prevention catheter-associated (CA) urinary tract infection (UTI), CAUTI remains one most common health care-related infections. We previously showed that an antimicrobial/antibiofilm agent inhibited biofilm development by Gram-positive and Gram-negative bacterial pathogens isolated from human In this study, we examined ability a novel preventative (BPA) coating on silicone catheters to inhibit formation six different UTIs: three Escherichia coli strains, representative bacterium UTI; Enterobacter cloacae, multidrug-resistant isolate; Pseudomonas aeruginosa, among patients with long-term catheterization; isolate methicillin-resistant Staphylococcus aureus, as both resistant organism. First, tested these strains form biofilms made red rubber, polyvinyl chloride (PVC), using microtiter plate assay. When grown in artificial urine medium, which closely mimics urine, all isolates formed considerable catheter materials. As biomass was 0.5 1.6 logs less than rubber or PVC, respectively, then coated BPA (benzalkonium chloride, polyacrylic acid, glutaraldehyde), further uropathogens. Compared uncoated catheters, BPA-coated completely prevented uropathogens, except P. no reduction biomass. To explore reason aeruginosa resistance coating, utilized two specific lipopolysaccharide (LPS) mutants. contrast their parent strain, mutants failed suggests composition LPS plays role wild-type coating. Together, our results suggest that, may prevent

Language: Английский

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Martini-3 Coarse-Grained Models for the Bacterial Lipopolysaccharide Outer Membrane of Escherichia coli

Journal of Chemical Theory and Computation, Journal Year: 2023, Volume and Issue: 20(4), P. 1704 - 1716

Published: Sept. 7, 2023

The outer lipopolysaccharide (LPS) membrane of Gram-negative bacteria forms the main barrier for transport antimicrobial molecules into bacterial cell. In this study we develop coarse-grained models

Language: Английский

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Electrochemical and Infrared Studies of a Model Bilayer of the Outer Membrane of Gram-Negative Bacteria and its Interaction with polymyxin─the Last-Resort Antibiotic

Langmuir, Journal Year: 2024, Volume and Issue: 40(15), P. 8248 - 8259

Published: April 5, 2024

A model bilayer of the outer membrane (OM) Gram-negative bacteria, composed lipid and 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE), was assembled on β-Tg modified gold (111) single crystal surface using a combination Langmuir–Blodgett Langmuir–Schaefer transfer. Electrochemical spectroscopic methods were employed to study properties its interaction with polymyxin. The is stable in transmembrane potential region between 0.0 −0.7 V. presence Mg2+ coordinates phosphate carboxylate groups leaflet stabilizes structure bilayer. Polymyxin causes leakage damage 0.2 −0.4 At potentials lower than −0.5 V, polymyxin does not affect integrity. binds molecules increase tilt angle acyl chains decrease C═O bond. results this paper indicate that antimicrobial activity depends at provides useful information for development new antibiotics.

Language: Английский

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Detection and photothermal inactivation of Gram-positive and Gram-negative bloodstream bacteria using photonic crystal biosensor and plasmonic core–shell

RSC Advances, Journal Year: 2024, Volume and Issue: 14(16), P. 11594 - 11603

Published: Jan. 1, 2024

Plasmonics and core-shell nanomaterials hold great potential to develop pharmaceuticals medical equipment due their eco-friendly cost effective fabrication procedures. Despite these advancements, combating drug-resistant bacterial infections remains a global challenge. Therefore, this study aims introduce tailored theoretical framework for one-dimensional (1D) photonic crystal biosensor (PCB) composed of (ZrO

Language: Английский

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What Spectro-electrochemical Studies Tell about the Interactions between Antimicrobial Peptides and Biomimetic Membranes at Electrode Surfaces

The Journal of Physical Chemistry C, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Language: Английский

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LPS-Dephosphorylating Cobetia amphilecti Alkaline Phosphatase of PhoA Family Divergent from the Multiple Homologues of Cobetia spp.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(3), P. 631 - 631

Published: March 21, 2024

A highly active alkaline phosphatase (ALP) of the protein structural family PhoA, from a mussel gut-associated strain marine bacterium Cobetia amphilecti KMM 296 (CmAP), was found to effectively dephosphorylate lipopolysaccharides (LPS). Therefore, aim this work perform comprehensive bioinformatics analysis structure, and suggest physiological role enzyme in bacteria genus Cobetia. scrutiny CmAP-like sequences 36 available genomes revealed nine homologues intrinsic subspecies C. amphilecti, whereas PhoA distant relative crustatorum JO1T carried an inactive mutation. However, phylogenetic all ALP showed that each possesses several variants, mostly genes encoding for PhoD PhoX families. The strains have complete set four families' genes, namely: PafA, PhoX, two structures. marina species is distinguished by presence only three genes. proteins are clustered together with human squid LPS-detoxifying enzymes. In addition, predicted biosynthesis gene cluster suggests its involvement control cellular redox balance, homeostasis, cell cycle. Apparently, variety ALPs spp. indicates significant adaptability phosphorus-replete depleted environments notable organophosphate destructor eco-niches which they once emerged, including Zostera clusterization degree similarity genus-specific biosynthetic ectoine polyketide T1PKS, responsible sulfated extracellular polysaccharide synthesis, coincide new whole genome-based taxonomic classification their suggested be adaptable use agriculture, biotechnology biomedicine.

Language: Английский

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The Effect of Lipopolysaccharides from Salmonella enterica on the Size, Density, and Compressibility of Phospholipid Vesicles

Biomimetics, Journal Year: 2025, Volume and Issue: 10(1), P. 55 - 55

Published: Jan. 15, 2025

The properties of the large unilamellar vesicles (LUVs) from 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), modified by lipopolysaccharides (LPS) Salmonella enterica sv. Enteritidis, which mimics Gram-negative bacteria, were studied various physical methods. LPS, in range 0/20/50 % w/w relative to lipid, had a regulatory role structure LUVs toward lower size, low polydispersity, and over-a-month size stability due negative zeta potential. addition LPS resulted increased density, determined ultrasound velocity specific adiabatic compressibility. In 0.5/1/2 mg/mL concentration range, total lipid content did not significantly affect influenced density-related attributes similarly content. A positive correlation was found between temperature vesicle density compressibility—except for anomaly behavior at 25 °C, around melting point DMPC.

Language: Английский

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The Biphasic Effect of Lipopolysaccharide on Membrane Potential

Membranes, Journal Year: 2025, Volume and Issue: 15(3), P. 74 - 74

Published: March 2, 2025

Lipopolysaccharide (LPS) from certain strains of Gram-negative bacteria can induce a rapid (<1 s) hyperpolarization membrane potential, followed by gradual depolarization exceeding the initial resting potential. Through overexpression Drosophila ORK1 two-pore-domain K+ channel (K2P) in larval muscles and altering external concentrations Na+ ions, it is clear that due to activating K2P channels promoting an inward leak. When concentration negligible, LPS-delayed dampened. The induced LPS exceed −100 mV when are lowered. These results indicate direct action on ion independently immune responses. Such actions may need be considered developing clinical treatments for forms bacterial septicemia.

Language: Английский

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Advanced antimicrobial surfaces in cellulose-based food packaging

Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 341, P. 103472 - 103472

Published: March 13, 2025

Language: Английский

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Bacterial Membrane Mimetics: From Biosensing to Disease Prevention and Treatment

Biosensors, Journal Year: 2023, Volume and Issue: 13(2), P. 189 - 189

Published: Jan. 26, 2023

Plasma membrane mimetics can potentially play a vital role in drug discovery and immunotherapy owing to the versatility assemble facilely cellular membranes on surfaces and/or nanoparticles, allowing for direct assessment of drug/membrane interactions. Recently, bacterial (BMs) have found widespread applications biomedical research as antibiotic resistance is rise, bacteria-associated infections become one major causes death worldwide. Over last decade, BM has greatly benefited from parallel advancements nanotechnology bioelectronics, resulting multifaceted systems variety sensing applications. As such, BMs coated electroactive are particularly promising label-free platform investigate interfacial phenomena, well interactions with drugs at first point contact: membrane. Another common approach suggests use lipid-coated nanoparticles carrier system therapies infectious diseases cancer. Herein, we discuss emerging platforms that make biosensing, bioimaging, delivery/discovery, immunotherapy, focusing Further, detail synthesis characteristics BMs, followed by various models utilizing them The key areas required augment facilitate wider applicability also touched upon. Overall, this review provides an interdisciplinary exploit potential current technologies generate novel solutions unmet clinical needs.

Language: Английский

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