One‐Pot Synthesis of [(l‐GluA)‐b‐(PCL)]‐Based Diblock Copolymer and Solvent‐Assisted Multifaceted Capsules as Potential Cargo Payload for Nanomedicines DOI
Chander Amgoth,

Raju Bura,

David Xu Luo

et al.

ChemistrySelect, Journal Year: 2024, Volume and Issue: 9(41)

Published: Oct. 28, 2024

Abstract This study shows that the synthesis of di‐BCP between amino acid l ‐glutamic ( ‐GluA) and hydrophobic polycaprolactone (PCL) leads to multifunctional multifaceted capsule formation with different dispersion solvents. Successful [( ‐GluA)‐b‐(PCL)] was confirmed by using FTIR 1H NMR characterizations. For morphological studies, synthesized ‐GluA)‐ b ‐(PCL)] dispersed in four solvents such as acetone, DMSO, IPA, ethanol used for sample preparation SEM imaging. Interestingly, smooth‐surfaced NPs (defined type I NPs), rough‐surfaced (type II porous III football‐type deep excavation having IV NPs). Furthermore, these were characterized through BET, AFM, DLS particle size ζ potential NPs. LC types calculated be 1.96% ± 0.01% 1.92% 0.01%, respectively. EE 91% 1.5% 94% 1.2%. We K562 (leukemia blood cancer) HEK293 (embryonic kidney) cells vitro studies. DOX@[( nanoformulation related significant early apoptosis is, 29.17%, which is a remarkable programmed cell death nanoparticles.

Language: Английский

Toward understanding lipid reorganization in RNA lipid nanoparticles in acidic environments DOI Creative Commons
Adiran Garaizar, Christian Díaz-Oviedo,

Nina Zablowsky

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(45)

Published: Oct. 30, 2024

The use of lipid nanoparticles (LNPs) for therapeutic RNA delivery has gained significant interest, particularly highlighted by recent milestones such as the approval Onpattro and two mRNA-based SARS-CoV-2 vaccines. However, despite substantial advancements in this field, our understanding structure internal organization RNA-LNPs -and their relationship to efficacy, both vitro vivo- remains limited. In study, we present a coarse-grained molecular dynamics (MD) approach that allows simulations full-size LNPs. By analyzing MD-derived structural characteristics conjunction with cellular experiments, investigate effect critical parameters, pH composition, on LNP potency. Additionally, examine mobility chemical environment within LNPs at level. Our findings highlight impact composition can have key stages LNP-based intracellular delivery.

Language: Английский

Citations

6
Medium-chain fatty acids and monoglycerides: Nanoarchitectonics-based insights into molecular self-assembly, membrane interactions, and applications DOI
Bo Kyeong Yoon, Joshua A. Jackman

Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 340, P. 103465 - 103465

Published: March 1, 2025

Language: Английский

Citations

0
Fast and reliable probabilistic reflectometry inversion with prior-amortized neural posterior estimation DOI Creative Commons
Vladimir Starostin, Maximilian Dax, Alexander Gerlach

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(11)

Published: March 14, 2025

Reconstructing the structure of thin films and multilayers from measurements scattered x-rays or neutrons is key to progress in physics, chemistry, biology. However, finding all structures compatible with reflectometry data computationally prohibitive for standard algorithms, which typically results unreliable analysis only a single potential solution identified. We address this lack reliability probabilistic deep learning method that identifies realistic seconds, redefining standards reflectometry. Our method, prior-amortized neural posterior estimation (PANPE), combines simulation-based inference adaptive priors inform network about known structural properties controllable experimental conditions. PANPE networks support scenarios such as high-throughput sample characterization, real-time monitoring evolving structures, corefinement several datasets can be adapted provide fast, reliable, flexible across many other inverse problems.

Language: Английский

Citations

0
Single-Particle Adsorption of Ultra-Small Gold Nanoparticles at the Biomembrane Phase Boundary DOI Creative Commons
Rashad Kariuki, Saffron J. Bryant,

T.J. Shepherd

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 253, P. 114734 - 114734

Published: April 24, 2025

Language: Английский

Citations

0
Advancements in Engineering Planar Model Cell Membranes: Current Techniques, Applications, and Future Perspectives DOI Creative Commons

Sara Coronado,

Johan Herrera,

María Graciela Pino

et al.

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(18), P. 1489 - 1489

Published: Sept. 13, 2024

Cell membranes are crucial elements in living organisms, serving as protective barriers and providing structural support for cells. They regulate numerous exchange communication processes between cells their environment, including interactions with other cells, tissues, ions, xenobiotics, drugs. However, the complexity heterogeneity of cell membranes-comprising two asymmetric layers varying compositions across different types states (e.g., healthy vs. diseased)-along challenges manipulating real represent significant obstacles vivo studies. To address these challenges, researchers have developed various methodologies to create model or membrane fragments, mono- bilayers organized planar systems. These models facilitate fundamental studies on component well components external agents, such drugs, nanoparticles (NPs), biomarkers. The applications extended beyond basic research, encompassing areas biosensing nanoparticle camouflage evade immune detection. In this review, we highlight advancements engineering membranes, focusing nanoarchitectonic tools used fabrication. We also discuss approaches incorporating challenging materials, proteins enzymes, into models. Finally, present our view future perspectives field membranes.

Language: Английский

Citations

0
One‐Pot Synthesis of [(l‐GluA)‐b‐(PCL)]‐Based Diblock Copolymer and Solvent‐Assisted Multifaceted Capsules as Potential Cargo Payload for Nanomedicines DOI
Chander Amgoth,

Raju Bura,

David Xu Luo

et al.

ChemistrySelect, Journal Year: 2024, Volume and Issue: 9(41)

Published: Oct. 28, 2024

Abstract This study shows that the synthesis of di‐BCP between amino acid l ‐glutamic ( ‐GluA) and hydrophobic polycaprolactone (PCL) leads to multifunctional multifaceted capsule formation with different dispersion solvents. Successful [( ‐GluA)‐b‐(PCL)] was confirmed by using FTIR 1H NMR characterizations. For morphological studies, synthesized ‐GluA)‐ b ‐(PCL)] dispersed in four solvents such as acetone, DMSO, IPA, ethanol used for sample preparation SEM imaging. Interestingly, smooth‐surfaced NPs (defined type I NPs), rough‐surfaced (type II porous III football‐type deep excavation having IV NPs). Furthermore, these were characterized through BET, AFM, DLS particle size ζ potential NPs. LC types calculated be 1.96% ± 0.01% 1.92% 0.01%, respectively. EE 91% 1.5% 94% 1.2%. We K562 (leukemia blood cancer) HEK293 (embryonic kidney) cells vitro studies. DOX@[( nanoformulation related significant early apoptosis is, 29.17%, which is a remarkable programmed cell death nanoparticles.

Language: Английский

Citations

0