Novel Salinity-Tolerant Third-Generation Hybrid Rice Developed via CRISPR/Cas9-Mediated Gene Editing

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(9), P. 8025 - 8025

Published: April 28, 2023

Climate change has caused high salinity in many fields, particularly the mud flats coastal regions. The resulting become one of most significant abiotic stresses affecting world's rice crop productivity. Developing elite cultivars with novel salinity-tolerance traits is regarded as cost-effective and environmentally friendly approach for utilizing saline-alkali land. To develop a highly efficient green strategy create germplasms salt-tolerant breeding, this study aimed to improve tolerance by combining targeted CRISPR/Cas9-mediated editing OsRR22 gene heterosis utilization. alleles genic male-sterility (GMS) restorer line (733Srr22-T1447-1 HZrr22-T1349-3) produced 110 1 bp deletions at third exon conferred level tolerance. Homozygous transgene-free progeny were identified via segregation T2 generation, osrr22 showing similar agronomic performance wild-type (733S HZ). Furthermore, these two lines used new promising third-generation hybrid Overall, results demonstrate that CRISPR/Cas9 "third-generation system" allows development varieties exhibit tolerance, thereby ensuring improved cultivar stability enhanced

Language: Английский

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Comparative transcriptome analysis of gene responses of salt-tolerant and salt-sensitive rice cultivars to salt stress

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Nov. 4, 2023

Salt stress is one unfavorable factor of global climate change that adversely affects rice plant growth and yield. To identify novel salt-tolerant genes new varieties rice, a better understanding the molecular regulation mechanism salt tolerance in needed. In this study we used transcriptome analyses to examine changes gene expression salt-sensitive plants. The cultivar HH11 IR29 were treated with 200 mM NaCl solution for 0 h, 6 24 h 48 at three leaf stage. Physiological parameters measured analyzed after each treatment. Activity SOD POD, as well MDA protein content two cultivars generally increased increasing time exposure NaCl. Meanwhile, APX activity first increased, then decreased both cultivars, maximum values seen HH11. GR GPX stronger than response stress. H2O2 0-6 6-24 again 24-48 under Compared IR29, SOD, POD was more sluggish stress, reaching or h. MDA, proline lower Relative untreated plants (0 h) those exposed (H0-H6, H0-H24 H0-H48), 7462, 6363 6636, differentially expressed (DEGs), respectively, identified. For respective total DEGs 7566, 6075 6136. GO KEGG enrichment analysis showed metabolic pathways related antioxidative responses osmotic balance played vital roles tolerance. Sucrose starch metabolism, addition flavonoid biosynthesis glutathione positive Expression SPS (LOC_Os01g69030 LOC_Os08g20660) GST (LOC_Os06g12290 LOC_Os10g38740) up-regulated whereas LOC_Os09g12660, glucose-1-phosphate adenylyltransferase gene, SS (LOC_Os04g17650 LOC_Os04g24430) differential results had favorable adjustment antioxidant upon highlighted candidate could play function rice.

Language: Английский

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Bacillus amyloliquefaciens Modulate Carbohydrate Metabolism in Rice-PGPR Cross-Talk Under Abiotic Stress and Phytohormone Treatments

Journal of Plant Growth Regulation, Journal Year: 2023, Volume and Issue: 42(7), P. 4466 - 4483

Published: Feb. 3, 2023

Language: Английский

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The rice LATE ELONGATED HYPOCOTYL enhances salt tolerance by regulating Na⁺/K⁺ homeostasis and ABA signalling

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: 47(5), P. 1625 - 1639

Published: Jan. 28, 2024

Abstract The circadian clock plays multiple functions in the regulation of plant growth, development and response to various abiotic stress. Here, we showed that core oscillator component late elongated hypocotyl (LHY) was involved rice salt mutations OsLHY gene led reduced tolerance rice. Transcriptomic analyses revealed regulates expression genes related ion homeostasis abscisic acid (ABA) signalling pathway, including encoded High‐affinity K + transporters ( OsHKTs ) stress‐activated protein kinases OsSAPKs ). We demonstrated directly binds promoters OsHKT1;1 , OsHKT1;4 OsSAPK9 regulate their expression. Moreover, ossapk9 mutants exhibited under Taken together, our findings integrates ABA pathway rice, providing insights into understanding how controls

Language: Английский

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Halotolerant Microorganism-Based Soil Conditioner Application Improved the Soil Properties, Yield, Quality and Starch Characteristics of Hybrid Rice under Higher Saline Conditions

Plants, Journal Year: 2024, Volume and Issue: 13(16), P. 2325 - 2325

Published: Aug. 21, 2024

Soil salinity represents a significant factor affecting agricultural productivity and crop quality. The present study was conducted to investigate the effects of soil conditioner (SC) comprising halotolerant microorganisms on fertility, yield, rice quality, physicochemical structural properties starch in hybrid under saline conditions. experimental treatments were composed two high-quality varieties, i.e., ‘Y Liangyou 957’ (YLY957) Jing 534 (JLY534), amendment treatments, application SC at control levels 2250 kg hm−2, or ‘CK SC’, respectively. subjected mixture fresh sea water (EC 11 dS/m). results demonstrated that significantly enhanced yield salt stress conditions owing an increase number grains per panicle. Furthermore, found be effective improving organic matter nutrient content. resulted improvement antioxidant defense, higher leaf SPAD values, greater biomass, as well translocation photo-assimilates heading stage. not only improved milling appearance quality but also taste value by increasing amylose reducing protein decreased indentations surfaces granules cracks edges granules. varieties exhibited excellent pasting properties, characterized reduced proportions amylopectin short chains lower gelatinization temperature enthalpy gelatinization. Overall, these findings serve reinforce efficacy valuable tool improve sustainability with grain

Language: Английский

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