TRANSPARENT TESTA 16 collaborates with the MYB-bHLH-WD40 transcriptional complex to produce brown fiber cotton DOI Creative Commons
Yuanxue Li, Tian Yao, Chao Fu

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 11, 2024

Naturally colored cotton (NCC; Gossypium spp.) does not require additional chemical dyeing and is an environmentally friendly textile material with great research potential applications. Our previous study using linkage association mapping identified TRANSPARENT TESTA 2 (Gh_TT2) as acting on the proanthocyanin synthesis pathway. However, limited information available about genetic regulatory network of NCC. Here, we verified effectiveness Gh_TT2 roles red foliated mutant gene (Re) in pigment formation deposition brown fiber (BFC). Variations derived from interspecific hybridization between barbadense acc. Pima 90-53 hirsutum Handan208 resulted expression differences, thereby causing phenotypic variation. Additionally, MYB-bHLH-WD complex was found to be negatively modulated by 16/ARABIDOPSIS BSISTER (TT16/ABS). RNA-seq suggested that differential homologous genes key enzymes pathway strongly contributes color rendering natural dark light cotton. proposes a model BFC, which will provide theoretical guidance for improvement

Language: Английский

Unveiling the power of MYB transcription factors: Master regulators of multi-stress responses and development in cotton DOI
Zhenzhen Wang, Zhen Peng,

Sana Khan

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 276, P. 133885 - 133885

Published: July 16, 2024

Language: Английский

Citations

14
Fine Mapping and Candidate Genes Analysis for Regulatory Gene of Anthocyanin Synthesis in the Corolla, Shedding Light on Wild Potato Evolution DOI Open Access
Zewei Zhang, Hongjun Li, Lingkui Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1966 - 1966

Published: Feb. 25, 2025

Petota includes more than 100 species (wild and cultivated), presenting a rich variety of corolla colors associated traits. This variability provides important opportunities for investigating the differentiation orthologous genes' functions their evolutionary pathways. However, genetic underpinnings this diversity in are still to be further explored. In our previous study, locus responsible color potato was mapped 740 kb region on chromosome 10, which contains AN2 gene previously identified as regulation color. present refined 380 interval through recombinant analysis. Targeted analysis anthocyanidins carotenoids revealed that purple corollas exhibit significantly higher levels petunidin delphinidin, while showing lower lutein β-carotene compared yellow corollas. Transcriptome qRT-PCR indicated StMYB180, rather AN2, is candidate regulating coloration, specifically abaxial side potato. Expression StMYB180 exclusively highly expressed leaf tissues, with coloration both leaves. Phylogenetic suggests color-regulatory genes may closely tied origin trajectory species. study valuable insights into tissue-specific expression anthocyanin biosynthesis lays groundwork understanding evolution section.

Language: Английский

Citations

0
MYB regulation of GST/GT mediates red petal spot development in cotton DOI Creative Commons
Sujun Zhang, Jie Chen, Tao Jiang

et al.

The Crop Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
The genetic basis of replicated bullseye pattern reduction across the Hibiscus trionum complex DOI Creative Commons
May T. S. Yeo, Alice L. M. Fairnie,

Valentina Travaglia

et al.

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: May 20, 2025

Summary Colorful petal patterns fulfill important functions and constitute excellent systems to illuminate the evolutionary processes that generate morphological diversity or instead support repetitive emergence of similar forms. Here, we combined phylogenomic approaches, genetic manipulations, molecular techniques, bee behavioral experiments (i) solve species relationships across Trionum complex, a small Hibiscus clade displays bullseye varying in size, hue, composition, (ii) identify key genes involved production pigmentation, (iii) reveal events underpinning pattern variation during evolution group. We found epidermal cell shape, texture, pigmentation are genetically distinct is most labile feature demonstrate reduction primarily occur through independent modifications single locus encoding BERRY1, an R2R3 MYB (myeloblastosis) regulates anthocyanin pigment petals. also buff‐tailed bumblebees discriminate against flowers with smaller sizes, suggesting changing proportions impact plant–pollinator interactions. Our results how repeated mutations led patterning, trait shown plant fitness other contribute angiosperm reproductive isolation speciation.

Language: Английский

Citations

0
Genetic analysis and molecular validation of gene conferring petal spot phenotype in interspecific crosses of cotton DOI Creative Commons

Salil Jindal,

Dharminder Pathak,

Tanvir Dutt

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(10), P. e31538 - e31538

Published: May 1, 2024

Cotton (

Language: Английский

Citations

2
An R2R3-MYB Transcriptional Factor LuMYB314 Associated with the Loss of Petal Pigmentation in Flax (Linum usitatissimum L.) DOI Open Access
Dongliang Guo, Haixia Jiang,

Liqiong Xie

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(4), P. 511 - 511

Published: April 18, 2024

The loss of anthocyanin pigments is one the most common evolutionary transitions in petal color, yet genetic basis for these changes flax remains largely unknown. In this study, we used crossing studies, a bulk segregant analysis, genome-wide association phylogenetic and transgenic testing to identify genes responsible transition from blue white petals flax. This study found no correspondence between color seed refuting conclusion that locus controlling coat associated with as reported previous studies. was mapped using BSA-seq analysis based on F

Language: Английский

Citations

1
Anthocyanin gene enrichment in the distal region of cotton chromosome A07: mechanisms of reproductive organ coloration DOI Creative Commons

Liuchang Zheng,

Jilong Zhang,

Haiyan He

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: April 18, 2024

The biosynthesis of secondary metabolites like anthocyanins is often governed by metabolic gene clusters (MGCs) in the plant ancestral genome. However, existence specifically regulating anthocyanin accumulation certain organs not well understood.

Language: Английский

Citations

1
R2R3-MYB Gene Family in Coptis teeta Wall.: Genome-Wide Identification, Phylogeny, Evolutionary Expansion, and Expression Analyses during Floral Development DOI Open Access

Ji-Chen Yu,

Shaofeng Duan,

Zhenyang Shua

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8902 - 8902

Published: Aug. 15, 2024

The

Language: Английский

Citations

1
Natural variations in the Cis‐elements of GhRPRS1 contributing to petal colour diversity in cotton DOI Creative Commons
Wei Hu, Yanli Chen, Zhenzhen Xu

et al.

Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 16, 2024

Summary The cotton genus comprises both diploid and allotetraploid species, the diversity in petal colour within this offers valuable targets for studying orthologous gene function differentiation evolution. However, genetic basis remains largely unknown. red primarily comes from C, G, K, D genome it is likely that common ancestor of had petals. Here, by employing a clone mapping strategy, we mapped trait to specific region on chromosome A07 upland cotton. Genomic comparisons phylogenetic analyses revealed phenotype introgressed G. bickii . Transcriptome analysis indicated GhRPRS1 , which encodes glutathione S‐transferase, was causative colour. Knocking out resulted white petals absence spots, while overexpression genotypes led Further suggested played role transporting pelargonidin‐3‐O‐glucoside cyanidin‐3‐O‐glucoside. Promoter activity variations promoter, but not body have different colours genus. Our findings provide new insights into evolution as well strategies modifying promoters breeding.

Language: Английский

Citations

1
Comparative Transcriptome Analysis Reveals Changes in Gene Expression Associated with Anthocyanin Metabolism in Begonia semperflorens under Light Conditions DOI Creative Commons
Kunkun Zhao,

Airong Liu,

Yuanbing Zhang

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(1), P. 96 - 96

Published: Jan. 19, 2024

Anthocyanins, recognized as stress indicators, particularly under high-light conditions, play a pivotal role in plant responses. The advent of transcriptomics has opened avenues to elucidate the mechanisms underlying high light-induced anthocyanin biosynthesis. This study delved into transcriptomic changes Begonia semperflorens leaves varying light intensities: 950–9600 lx (TL_100), 6800–7000 (HS_75), and 4300–4500 (LS_25). To confirm expression profiles key genes, we chose 12 critical genes associated with production for quantitative reverse transcription PCR (qRT-qPCR) analysis. Following this, measured levels anthocyanins substantiate findings from gene transcriptome assembly this was extensive, yielding 43,038 unigenes that collectively spanned about 49.83 million base pairs, an average unigene length 1157 bp N50 value 1685 bp. facilitated thorough functional annotation across seven distinct protein databases, leading classification 16,363 58 different families factors. Our comparative analysis transcriptomes highlighted substantial number differentially expressed (DEGs): 5411 DEGs between HS_75 TL_100 3078 showing increased 2333 decreased expression; 4701 LS_25 TL_100, consisting 2648 up-regulated 2053 down-regulated genes; 6558 HS_75, 3032 3526 down-regulated. These were significantly involved pathways, such synthesis, hormone signaling, other regulatory mechanisms. suggests including F3′H, MYB102, SWEET1, could vital roles regulating synthesis response various potentially impacting like WRKYs, ATHB12, those similar HSP.

Language: Английский

Citations

0