Transcriptomic and Metabolomic Analyses Reveal the Response to Short-Term Drought Stress in Bread Wheat (Triticum aestivum L.)

Agronomy, Journal Year: 2024, Volume and Issue: 14(4), P. 704 - 704

Published: March 28, 2024

Drought stress, a major abiotic significantly affects wheat (Triticum aestivum L.) production globally. To identify genes and metabolic pathways crucial for responding to short-term drought we conducted transcriptomic metabolomic analyses of winter cultivar Jimai 418 at four developmental stages: jointing (GS31), booting (GS45), anthesis (GS65), 8 days after (DAA8). Transcriptomic analysis identified 14,232 differentially expressed (DEGs) under stress compared the control. Specifically, 1387, 4573, 7380, 892 DEGs were stages, respectively. Enriched associated with these included plant hormone signal transduction, mitogen-activated protein kinase (MAPK) signaling, galactose metabolism, starch sucrose metabolism. Totals 222, 633, 358, 38 accumulated metabolites (DAMs) Correlation both datasets revealed DAMs arginine proline ABC transporters, amino acid biosynthesis. These findings offer significant insights into 418’s molecular response stress. The DEGs, DAMs, enriched contribute our understanding tolerance. This research will facilitate further investigations tolerance mechanisms guide breeding varieties enhanced resistance.

Language: Английский

Changes in Allele Frequencies and Genetic Diversity in Red Clover after Selection for Cold Tolerance Using SSR Markers

Agriculture, Journal Year: 2023, Volume and Issue: 13(10), P. 2019 - 2019

Published: Oct. 18, 2023

The selection of red clover (Trifolium pratense L.) populations adapted to extreme environmental conditions is great importance due continuing climate change. plant material analyzed with simple sequence repeat (SSR) markers included two parent populations, P1 (cultivar ‘Reichersberger’) and P3 ‘Croatia’) their reselections, which were created after one cycle under cold temperature conditions. reselections PS1 PS3 produced by intercrossing 38 surviving plants P3, respectively. A total 48 from each cultivar reselection randomly selected for SSR analysis. Sixteen primer pairs selected, taking into account the presence loci on all seven chromosomes. An increase in average frequency alleles initial was observed, followed a decrease number alleles. Out 16 loci, Waples neutrality test revealed significant changes at 12 9 populations. genetic diversity studied did not change significantly selection, leaving enough variability as prerequisite success future selection.

Language: Английский