Characterization of a 4.1 Mb inversion harboring the stripe rust resistance gene YR86 on wheat chromosome 2AL DOI Creative Commons
Qiang Cao, Zhanwang Zhu, Dengan Xu

et al.

The Crop Journal, Journal Year: 2024, Volume and Issue: 12(4), P. 1168 - 1175

Published: June 13, 2024

Wheat cultivar Zhongmai 895 was earlier found to carry YR86 in an 11.6 Mb recombination-suppressed region on chromosome 2AL when crossed with Yangmai 16. To fine-map the locus, we developed two large F2 populations from crosses Emai 580/Zhongmai and Avocet S/Zhongmai 895. Remarkably, both exhibited suppressed recombination same region. Collinearity analysis across Chinese Spring, Aikang 58, 10+ wheat genomes revealed a 4.1 chromosomal inversion spanning 708.5–712.6 Spring reference genome. Molecular markers were breakpoint used assess panel, revealing that 895, Jimai 22 shared common sequence named InvCS, whereas 16, 580, S InvAK58. The inverted configuration explained observed all three bi-parental populations. Normal 22/Zhongmai population, facilitating mapping of genetic interval 0.15 cM corresponding 710.27–712.56 falling within Thirty-three high-confidence genes annotated using genome, six identified as potential candidates for based genome transcriptome analyses. These results will accelerate map-based cloning its deployment breeding.

Language: Английский

Identification of 39 stripe rust resistance loci in a panel of 465 winter wheat entries presumed to have high-temperature adult-plant resistance through genome-wide association mapping and marker-assisted detection DOI Creative Commons

Fangjie Yao,

Meinan Wang, Deven R. See

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 7, 2025

Stripe rust of wheat is a serious disease caused by Puccinia striiformis f. sp. tritici (Pst). Growing resistant cultivars the most preferred approach to control disease. To identify genotypes with quantitative trait loci (QTL) for durable resistance stripe rust, 465 winter entries that were presumed have high-temperature adult-plant (HTAP) used in this study. In greenhouse seedling tests seven Pst races, 16 all tested races. The also phenotyped responses at stage under natural infection multiple field locations from 2018 2021 Washington state, and 345 found stable resistance. contrast susceptibility indicated predominantly HTAP panel. durability was demonstrated subset 175 2007 2021. genotyped through genotyping multiplexed sequencing single-nucleotide polymorphism (SNP) markers. Combining response SNP marker data, genome-wide association study (GWAS) conducted, resulting 143 marker-trait associations, which 28 QTL detected least two races or environments identified, including all-stage 21 These each explained 6.0% 40.0% phenotypic variation. Compared previously reported Yr genes based on their genomic positions, five identified as new. A total 10 user-friendly Kompetitive allele specific PCR (KASP) markers developed eight loci. addition, molecular detect 13 genes/QTL, GWAS analyses, frequencies ranged 0.86% 88.17% genotypes, genes/QTL KASP should be useful develop long-lasting rust.

Language: Английский

Citations

0
Genetic Basis of Resistance to Wheat Yellow Rust DOI

Т. В. Чугункова,

N. L. Pastukhova, Ya. V. Pirko

et al.

Cytology and Genetics, Journal Year: 2025, Volume and Issue: 59(2), P. 186 - 196

Published: April 1, 2025

Language: Английский

Citations

0
Characterization of a 4.1 Mb inversion harboring the stripe rust resistance gene YR86 on wheat chromosome 2AL DOI Creative Commons
Qiang Cao, Zhanwang Zhu, Dengan Xu

et al.

The Crop Journal, Journal Year: 2024, Volume and Issue: 12(4), P. 1168 - 1175

Published: June 13, 2024

Wheat cultivar Zhongmai 895 was earlier found to carry YR86 in an 11.6 Mb recombination-suppressed region on chromosome 2AL when crossed with Yangmai 16. To fine-map the locus, we developed two large F2 populations from crosses Emai 580/Zhongmai and Avocet S/Zhongmai 895. Remarkably, both exhibited suppressed recombination same region. Collinearity analysis across Chinese Spring, Aikang 58, 10+ wheat genomes revealed a 4.1 chromosomal inversion spanning 708.5–712.6 Spring reference genome. Molecular markers were breakpoint used assess panel, revealing that 895, Jimai 22 shared common sequence named InvCS, whereas 16, 580, S InvAK58. The inverted configuration explained observed all three bi-parental populations. Normal 22/Zhongmai population, facilitating mapping of genetic interval 0.15 cM corresponding 710.27–712.56 falling within Thirty-three high-confidence genes annotated using genome, six identified as potential candidates for based genome transcriptome analyses. These results will accelerate map-based cloning its deployment breeding.

Language: Английский

Citations

2