Mechanical stress contributes to ligamentum flavum hypertrophy by inducing local inflammation and myofibroblast transition in the innovative surgical rabbit model DOI Creative Commons

Qinghong Ma,

Xinyu Feng,

Yongxin Chen

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 15, 2025

Background Lumbar spinal canal stenosis (LSCS) ranks as a prevalent disorder in senior populations. Ligamentum flavum hypertrophy (LFH) is significant feature of LSCS, yet its cause unclear. The purpose this study was to create novel animal model for LFH and explore the pathological mechanisms involved. Methods A rabbit intervertebral mechanical stress concentration established through posterolateral fusion using steel wire. Radiological analysis biological validation were used determine crucial role effect model. Results After 12 weeks, LF subjected exhibited disruption reduction elastic fibers, collagen accumulation, increased thickness LF, elevated cells, levels certain factors related fibrosis inflammation. These findings histologically consistent those found human LFH. Furthermore, vitro , stretch discovered enhance conversion fibroblasts into myofibroblasts by boosting TGF-β1 secretion fibroblasts. In addition, compared conventional internal fixation, new surgical provided advantages such minor damage, decreased bleeding, reduced technical difficulty molding costs. Conclusion This able replicate moderate features Mechanical an independent factor leading LFH, which can promote cells some inflammatory subsequently induce myofibroblast transition, finally result accumulation fibrosis.

Language: Английский

Mechanical stress contributes to ligamentum flavum hypertrophy by inducing local inflammation and myofibroblast transition in the innovative surgical rabbit model DOI Creative Commons

Qinghong Ma,

Xinyu Feng,

Yongxin Chen

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 15, 2025

Background Lumbar spinal canal stenosis (LSCS) ranks as a prevalent disorder in senior populations. Ligamentum flavum hypertrophy (LFH) is significant feature of LSCS, yet its cause unclear. The purpose this study was to create novel animal model for LFH and explore the pathological mechanisms involved. Methods A rabbit intervertebral mechanical stress concentration established through posterolateral fusion using steel wire. Radiological analysis biological validation were used determine crucial role effect model. Results After 12 weeks, LF subjected exhibited disruption reduction elastic fibers, collagen accumulation, increased thickness LF, elevated cells, levels certain factors related fibrosis inflammation. These findings histologically consistent those found human LFH. Furthermore, vitro , stretch discovered enhance conversion fibroblasts into myofibroblasts by boosting TGF-β1 secretion fibroblasts. In addition, compared conventional internal fixation, new surgical provided advantages such minor damage, decreased bleeding, reduced technical difficulty molding costs. Conclusion This able replicate moderate features Mechanical an independent factor leading LFH, which can promote cells some inflammatory subsequently induce myofibroblast transition, finally result accumulation fibrosis.

Language: Английский

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