PeerJ,
Journal Year:
2023,
Volume and Issue:
11, P. e16119 - e16119
Published: Sept. 28, 2023
The
quest
for
microbial
cellulases
has
intensified
as
a
response
to
global
challenges
in
biofuel
production.
efficient
deconstruction
of
lignocellulosic
biomass
holds
promise
generating
valuable
products
various
industries
such
food,
textile,
and
detergents.
This
article
presents
systematic
bioprospection
aimed
at
isolating
actinomycetes
with
exceptional
cellulose
capabilities.
Our
methodology
explored
the
biodiverse
oligotrophic
region
Cuatro
Cienegas,
Coahuila,
within
Chihuahuan
Desert.
Among
evaluated
collection,
78%
exhibited
cellulolytic
activity.
Through
meticulous
screening
process
based
on
enzymatic
index
evaluation,
we
identified
highly
Streptomyces
strain
further
investigation.
Submerged
fermentation
this
revealed
an
endoglucanase
activity
149
U/mg.
Genomic
analysis
sp.
STCH565-A
unique
configurations
carbohydrate-active
enzyme
(CAZyme)
genes,
underscoring
its
potential
bioconversion
applications.
These
findings
not
only
highlight
significance
Desert
rich
source
microorganisms
but
also
offer
insights
into
exploration
selection
high-performing
application
diverse
environmental
contexts.
In
conclusion,
our
bioprospecting
study
lays
foundation
harnessing
from
Desert,
implications
advancing
processes
industries.
can
serve
blueprint
future
efforts
different
regions,
facilitating
targeted
discovery
cellulosic
Notulae Scientia Biologicae,
Journal Year:
2023,
Volume and Issue:
15(2), P. 11490 - 11490
Published: June 19, 2023
Rapid
industrialization
and
consumerism
have
aggravated
the
generation
of
industrial
waste
globally,
consequently
posing
a
serious
problem
related
to
their
treatment,
disposal,
management.
Industrial
wastes
or
sludges
are
mainly
characterised
by
undesirable
levels
heavy
metals,
toxic
chemicals,
other
organic
compounds.
Deposition
such
in
environmental
matrices
for
prolonged
periods
may
result
contamination,
consequent
accumulation
these
harmful
constituents
ecological
food
chain.
Unavailability
appropriate
disposal
mechanisms
is
matter
concern
that
could
severely
pollute
environment
risk
human
health.
Vermicomposting
has
emerged
as
feasible
environmentally
friendly
bioremediation
technology
provide
solution
this
problem.
However,
vermicomposting
requires
better
understanding
its
inextricable
factors
make
it
viable
process.
Thus,
present
study
was
undertaken
insights
on
influence
different
bulking
agents
abiotic
process,
well
as,
role
earthworm
species
successful
implementation
process
waste.
Current Research in Biotechnology,
Journal Year:
2024,
Volume and Issue:
8, P. 100237 - 100237
Published: Jan. 1, 2024
Plastic
waste
has
become
a
significant
global
ecological
concern
due
to
its
substantial
harmful
impacts.
They
are
eventually
colonized
and
transformed
by
diverse
microbial
communities
known
as
the
''plastisphere".
Only
limited
group
of
microbes
have
been
identified
so
far
prevalence
non-culturable
strains
that
inhabit
polymer
substrates.
We
investigated
community
diversity
predictive
functionality
plastisphere
microbiota
using
high-throughput
next-generation
sequencing
(NGS)
approach
unveil
their
plastic
degrading
potential.
Samples
were
collected
from
four
different
plastic-enriched
disposal
sites
adjacent
Kolkata,
West
Bengal,
India,
followed
processing,
gDNA
extraction,
library
preparation,
sequencing.
The
most
prevalent
phyla
Proteobacteria,
Bacteroidetes,
Actinobacteria,
Cyanobacteria,
Firmicutes,
Verrucomicrobia.
functional
profiling
revealed
genes
associated
with
metabolism,
cellular
processes,
signalling
prevalent,
poorly
characterized
information
storage
processing.
In
this
study,
we
predicted
existence
beneficial
microbiome
bioremediation
degradation,
which
suggests
potential
utilization
plastics
primary
carbon
sources.
Our
findings
also
highlighted
promising
enzymes
biodegradation
several
Furthermore,
our
research
unveiled
enriched
distribution
in
studied
metagenome,
offers
prospect.
This
investigation
establishes
connection
between
structure
actively
engaged
within
sites.
These
degraders
can
be
further
for
broad-spectrum
applications
bioremediation.
Subsequent
explorations
will
provide
profound
contributions
pollution
mitigation.
PeerJ,
Journal Year:
2023,
Volume and Issue:
11, P. e16119 - e16119
Published: Sept. 28, 2023
The
quest
for
microbial
cellulases
has
intensified
as
a
response
to
global
challenges
in
biofuel
production.
efficient
deconstruction
of
lignocellulosic
biomass
holds
promise
generating
valuable
products
various
industries
such
food,
textile,
and
detergents.
This
article
presents
systematic
bioprospection
aimed
at
isolating
actinomycetes
with
exceptional
cellulose
capabilities.
Our
methodology
explored
the
biodiverse
oligotrophic
region
Cuatro
Cienegas,
Coahuila,
within
Chihuahuan
Desert.
Among
evaluated
collection,
78%
exhibited
cellulolytic
activity.
Through
meticulous
screening
process
based
on
enzymatic
index
evaluation,
we
identified
highly
Streptomyces
strain
further
investigation.
Submerged
fermentation
this
revealed
an
endoglucanase
activity
149
U/mg.
Genomic
analysis
sp.
STCH565-A
unique
configurations
carbohydrate-active
enzyme
(CAZyme)
genes,
underscoring
its
potential
bioconversion
applications.
These
findings
not
only
highlight
significance
Desert
rich
source
microorganisms
but
also
offer
insights
into
exploration
selection
high-performing
application
diverse
environmental
contexts.
In
conclusion,
our
bioprospecting
study
lays
foundation
harnessing
from
Desert,
implications
advancing
processes
industries.
can
serve
blueprint
future
efforts
different
regions,
facilitating
targeted
discovery
cellulosic
