Recovery of High-Value Compounds from Yarrowia lipolytica IMUFRJ 50682 Using Autolysis and Acid Hydrolysis DOI Open Access
Rhonyele Maciel da Silva, Bernardo Dias Ribeiro, Ailton César Lemes

et al.

Processes, Journal Year: 2024, Volume and Issue: 12(6), P. 1132 - 1132

Published: May 30, 2024

This study aimed to evaluate the sequential hydrolysis of biomass from unconventional and versatile Y. lipolytica recover mannoproteins, carbohydrates, other compounds as well determine antioxidant activity ultrafiltered fractions. The crude underwent autolysis, resulting supernatant fraction was used for mannoprotein recovery via precipitation with ethanol. precipitate obtained after autolysis acid hydrolysis, ultrafiltered, precipitated, characterized. process yields were 55.5% 46.14% grown in glucose glycerol, respectively. higher carbohydrate content (from glycerol) exhibited a emulsification index 47.35% thermal stability (60% weight loss). In contrast, protein glucose) showed better surface tension reduction 44.50 mN/m. technological properties that food ingredients are feasible apply processing. fractionation portion allowed evaluation power synergism among components present hydrolysate, mostly peptide chain. method is viable extracting valuable products lipolytica.

Language: Английский

Acoholysis behavior of PET waste with hybrid fibers and their application in the synthesis of unsaturated resins DOI
Juhua Ou,

Zilin Dai,

Zhenxing Kong

et al.

Journal of Polymer Research, Journal Year: 2025, Volume and Issue: 32(4)

Published: March 22, 2025

Language: Английский

Citations

0
Exploring yeast biodiversity and process conditions for optimizing ethylene glycol conversion into glycolic acid DOI Creative Commons
Vittorio Giorgio Senatore, Riccardo Milanesi,

Fiorella Masotti

et al.

FEMS Yeast Research, Journal Year: 2024, Volume and Issue: 24

Published: Jan. 1, 2024

Plastics have become an indispensable material in many fields of human activities, with production increasing every year; however, most the plastic waste is still incinerated or landfilled, and only 10% new recycled even once. Among all plastics, polyethylene terephthalate (PET) produced polyester worldwide; ethylene glycol (EG) one two monomers released by biorecycling PET. While research focuses on bacterial EG metabolism, this work reports ability Saccharomyces cerevisiae nine other common laboratory yeast species not to consume EG, but also produce glycolic acid (GA) as main by-product. A two-step bioconversion GA S. was optimized a design experiment approach, obtaining 4.51 ± 0.12 g l-1 conversion 94.25 1.74% from 6.21 0.04 EG. To improve titer, screening biodiversity identified Scheffersomyces stipitis best producer, 23.79 1.19 (yield 76.68%) bioreactor fermentation, single-step bioprocess. Our findings contribute laying ground for upcycling strategies yeasts.

Language: Английский

Citations

2
Biodepolymerization of Polyamide Fibers Using Yarrowia lipolytica as Whole-Cell Biocatalyst DOI Creative Commons
Adriano Carniel, Nathália Ferreira dos Santos, Filipe Smith Buarque

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(5), P. 239 - 239

Published: April 30, 2024

Polyamide is a thermoplastic polymer widely used for several applications, including cables in offshore oil and gas operations. Due to its growing annual production worldwide, this poorly biodegradable material has been source of pollution. Given scenario, the need arisen develop environmentally friendly techniques degrade waste, biotechnology emerged as possible solution mitigate problem. This study aimed investigate potential Yarrowia lipolytica biodepolymerize polyamide fibers (PAF). Microbial cultures were grown shaken flasks containing different concentrations PAF (0.5 2 g·L−1) bioreactor with without pH adjustment. mass loss was up 16.8%, achieved after 96 h cultivation Additionally, NMR analyses revealed that amorphous regions PAF, which are more susceptible depolymerization, reduced by 6% during cultivation. These preliminary results indicate biotechnological Y. depolymerize PAF.

Language: Английский

Citations

1
Recovery of High-Value Compounds from Yarrowia lipolytica IMUFRJ 50682 Using Autolysis and Acid Hydrolysis DOI Open Access
Rhonyele Maciel da Silva, Bernardo Dias Ribeiro, Ailton César Lemes

et al.

Processes, Journal Year: 2024, Volume and Issue: 12(6), P. 1132 - 1132

Published: May 30, 2024

This study aimed to evaluate the sequential hydrolysis of biomass from unconventional and versatile Y. lipolytica recover mannoproteins, carbohydrates, other compounds as well determine antioxidant activity ultrafiltered fractions. The crude underwent autolysis, resulting supernatant fraction was used for mannoprotein recovery via precipitation with ethanol. precipitate obtained after autolysis acid hydrolysis, ultrafiltered, precipitated, characterized. process yields were 55.5% 46.14% grown in glucose glycerol, respectively. higher carbohydrate content (from glycerol) exhibited a emulsification index 47.35% thermal stability (60% weight loss). In contrast, protein glucose) showed better surface tension reduction 44.50 mN/m. technological properties that food ingredients are feasible apply processing. fractionation portion allowed evaluation power synergism among components present hydrolysate, mostly peptide chain. method is viable extracting valuable products lipolytica.

Language: Английский

Citations

0