Cytotechnology, Journal Year: 2024, Volume and Issue: 77(1)
Published: Dec. 30, 2024
Language: Английский
Poultry Science, Journal Year: 2025, Volume and Issue: 104(5), P. 105003 - 105003
Published: March 7, 2025
Fatty liver hemorrhage syndrome (FLHS) is the most common metabolic diseases in laying hens during late-laying period, and it causes a significant economic burden on poultry industry. The competing endogenous RNA plays crucial roles occurrence development of fatty liver. Based previously constructed lncRNA-miRNA-mRNA networks, we selected axis ENSGALT00000079786-LPL-miR-143-5p for further study to elucidate its mechanistic role In this study, identified novel highly conserved lncRNA (ENSGALT00000079786) poultry, which designated as A2ml2 based chromosomal location. Fluorescent situ hybridization (FISH) revealed that was localized both nucleus cytoplasm. Dual-luciferase reporter assay validated targeted relationship between A2ml2, miR-143-5p, LPL gene. To analyze miR-143-5p function, overexpression vector successfully transfected into Leghorn male hepatocellular (LMH) cells, could remarkably inhibit cellular lipid deposition detected by oil red staining (P < 0.01), opposite occurred 0.01). qPCR demonstrated an inverse correlation expression expression, confirmed directly target A2ml2. Similarly, found miR-143-5p. investigate interactions among these three factors their effects metabolism, assessed levels co-transfecting with mimic binding site mutants. Co-transfection experiments showed diminished promoting effect LPL. Meanwhile, has capacity mitigate suppressive impact accumulation LMH cells. results attenuated hepatic through negatively regulating enhancing Our findings offer insights ceRNA-mediated FLHS identify potential molecular biomarker.
Language: Английский
Citations
0
International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(10), P. 3802 - 3822
Published: Jan. 1, 2024
Chronic tissue injury triggers changes in the cell type and microenvironment at site of eventually fibrosis develops.Current research suggests that is a highly dynamic reversible process, which means human intervention after has occurred potential to slow down or cure fibrosis.The ubiquitin system regulates biological functions specific proteins involved development fibrosis, researchers have designed small molecule drugs treat fibrotic diseases on this basis, but their therapeutic effects are still limited.With molecular biology technology, found non-coding RNA (ncRNA) can interact with jointly regulate fibrosis.More in-depth explorations interaction between ncRNA will provide new ideas for clinical treatment diseases.
Language: Английский
Citations
1
Cellular Signalling, Journal Year: 2024, Volume and Issue: 124, P. 111430 - 111430
Published: Sept. 21, 2024
Language: Английский
Citations
1
Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(18)
Published: Sept. 1, 2024
Abstract Oral submucous fibrosis (OSF) is a precancerous condition in the oral cavity, which closely related to myofibroblast conversion of buccal mucosal fibroblasts (BMFs) after chronic consumption areca nut. Emerging evidence suggests pyroptosis, form programmed cell death that mediated by inflammasome, implicated persistent activation and fibrosis. Besides, numerous studies have demonstrated effects non‐coding RNAs on pyroptosis activities. Herein, we aimed target key long RNA PVT1 with natural compound, carvacrol, alleviate OSF. We first identified was downregulated carvacrol‐treated fBMFs then features expression makers were all reduced response carvacrol treatment. Subsequently, analysed found aberrantly upregulated OSF specimens positively correlated several markers. After revealing suppressive characterisitcs repression PVT1, explored potential mechanisms. Our data showed may serve as sponge microRNA(miR)‐20a mitigate pyroptosis. Altogether, these findings indicated anti‐fibrosis merit consideration be due attenuation targeting PVT1/miR‐20a axis.
Language: Английский
Citations
1
Cytotechnology, Journal Year: 2024, Volume and Issue: 77(1)
Published: Dec. 30, 2024
Language: Английский
Citations
0