Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(11), P. 4787 - 4793
Published: March 11, 2022
Bladder
cancer
(BC)
is
one
of
the
most
common
cancers
in
world,
with
high
morbidity
and
mortality.
It
essential
to
develop
a
non-invasive,
highly
accurate,
simple
method
for
BC
diagnosis.
This
work
proposed
fluorescent
biosensor
based
on
inorganic
nanoflares
combined
DNAzyme
walker
simultaneous
detection
exosomal
microRNAs
(miRNAs).
was
constructed
Au
nanoparticle
(AuNP)
modified
carbon
dot
(CD)-labeled
substrates
strands
(AuNP@CDs
nanoflares-DNAzyme,
APCD).
In
presence
target
miRNAs,
activated
then
cleaved
CD-labeled
automatically
walked
along
AuNP,
allowing
fluorescence
recovery.
Due
structure
functional
composition,
APCD
biosensors
demonstrated
sensitivity
specificity,
reached
limit
single
miRNA
at
femtomolar
level
wide
linear
range
from
50
fM
10
nM.
Furthermore,
analysis
BC-related
miR-133b
miR-135b
clinical
serum
specimens
achieved
consistent
qRT-PCR,
suggesting
it
potential
diagnosis
other
cancers.
International Journal for Parasitology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Cystoisospora
suis,
a
porcine
enteral
parasite
of
the
order
Coccidia,
is
characterized
by
complex
life
cycle,
with
asexual
and
sexual
development
in
epithelium
host
gut
an
environmental
phase
as
oocyst.
All
developmental
stages
vary
greatly
their
morphology
function,
therefore
excrete
different
bioactive
molecules
for
intercellular
communication.
Due
to
development,
we
hypothesized
that
extracellular
vesicles
(EVs)
cargo
highly
dependent
on
cycle
from
which
they
are
released.
This
study
aimed
characterize
compare
EVs
all
C.
suis.
Nanoparticle
tracking
analysis
microscopy
were
used
determine
particle
numbers
size
distributions
stage-specific
EVs.
Furthermore,
Fourier-transform
infrared
spectral
was
employed
metabolic
fingerprinting
EVs,
lipid
protein
profiles
determined.
Overall,
revealed
asexual,
transmissible
suis
release
during
parasite's
cycle.
endogenous
found
be
more
similar
each
other
than
those
stage,
ratio
fatty
acids
polysaccharides
proteins
changed
development.
In
particular,
associated
Apicomplexa
involved
vesicle
shedding
showed
changes
expression
stages.
Lipid
same
concentration
through
stages,
whereas
amount
stereolipids,
sphingolipids
glycerolipids
between
conclusion,
this
study,
presents
first
known
characterization
demonstrates
link
respective
parasite,
putative
functions
parasite-parasite
host-parasite
interplays.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2022,
Volume and Issue:
10
Published: Jan. 31, 2022
Diabetic
wounds
remain
a
great
challenge
for
clinicians
due
to
the
multiple
bacterial
infections
and
oxidative
damage.
Exosomes,
as
an
appealing
nanodrug
delivery
system,
have
been
widely
applied
in
treatment
of
diabetic
wounds.
Endovascular
cells
are
important
component
vascular
wall.
Herein,
we
investigated
effects
HUCMSCs
HUC-Exos
(exosomes
secreted
by
HUCMSCs)
on
wound
healing.
In
this
study,
HUVECs
were
coincubated
with
HUCMSCs,
utilized
vitro
vivo
experiments
verify
their
roles
regulation
Our
results
demonstrated
that
ability
regulate
stress
injuries
endothelial
through
exosomes
accelerate
cutaneous
healing
vitro.
The
present
study
suggests
healing,
providing
promising
therapeutic
strategy
chronic
repair.
Small,
Journal Year:
2022,
Volume and Issue:
18(34)
Published: July 8, 2022
Severe
inflammation
and
myogenic
differentiation
disorder
are
the
major
obstacles
to
skeletal
muscle
healing
after
injury.
MicroRNAs
(miRNAs)
play
an
important
role
as
regulatory
molecules
during
process
of
healing,
but
detailed
mechanism
miRNA-mediated
intercellular
communication
between
myoblasts
macrophages
remains
unclear.
Here,
it
is
reported
that
secrete
miRNAs-enriched
exosomes
in
inflammatory
environment,
through
which
miR-224
transferred
into
inhibit
M2
polarization.
Further
data
demonstrate
WNT-9a
may
be
a
direct
target
for
macrophage
In
turn,
secretome
M1
impairs
promotes
proliferation.
Single-cell
integration
analysis
suggests
elevation
exosome-derived
caused
by
activation
key
factor
E2F1
demonstrates
RB/E2F1/miR-224/WNT-9a
axis.
vivo
results
show
treatment
with
antagomir-224
or
liposomes
containing
inhibitors
suppresses
fibrosis
improves
recovery.
These
findings
indicate
importance
crosstalk
via
miRNA-containing
regulation
polarization
differentiation/proliferation
healing.
This
study
provides
strategy
treating
injury
designing
polarization-enabling
anti-inflammatory
miRNA-based
bioactive
material.
Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(11), P. 4787 - 4793
Published: March 11, 2022
Bladder
cancer
(BC)
is
one
of
the
most
common
cancers
in
world,
with
high
morbidity
and
mortality.
It
essential
to
develop
a
non-invasive,
highly
accurate,
simple
method
for
BC
diagnosis.
This
work
proposed
fluorescent
biosensor
based
on
inorganic
nanoflares
combined
DNAzyme
walker
simultaneous
detection
exosomal
microRNAs
(miRNAs).
was
constructed
Au
nanoparticle
(AuNP)
modified
carbon
dot
(CD)-labeled
substrates
strands
(AuNP@CDs
nanoflares-DNAzyme,
APCD).
In
presence
target
miRNAs,
activated
then
cleaved
CD-labeled
automatically
walked
along
AuNP,
allowing
fluorescence
recovery.
Due
structure
functional
composition,
APCD
biosensors
demonstrated
sensitivity
specificity,
reached
limit
single
miRNA
at
femtomolar
level
wide
linear
range
from
50
fM
10
nM.
Furthermore,
analysis
BC-related
miR-133b
miR-135b
clinical
serum
specimens
achieved
consistent
qRT-PCR,
suggesting
it
potential
diagnosis
other
cancers.
