Identifying Aberrant 1CM-Related Pathways by Multi-Omics Analysis and Validating Tumor Inhibitory Effect of One-Carbon Donor Betaine in Gastric Cancer

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3841 - 3841

Published: April 18, 2025

Metabolic reprogramming, a well-established hallmark of gastric carcinogenesis, has been implicated in driving tumor progression. Nevertheless, the precise mechanisms through which these metabolic alterations orchestrate cancer (GC) pathogenesis remain incompletely elucidated. We conducted metabolomic analyses plasma samples obtained from 334 patients with GC and healthy individuals to identify differential metabolites pathways. Transcriptome sequencing was on six pairs tissues, joint analysis transcriptome metabolome performed. Single-cell data were acquired co-analyzed metabolomics investigate abnormalities at single-cell level. Finally, four representative selected using Random Forest subjected cellular experiments elucidate exert their effects. Metabolomic revealed that serine glycine metabolism, glycolysis, glutamate metabolism significantly altered GC, suggesting one-carbon (1CM)-related pathways are aberrantly activated. A combined transcriptome, indicated related oxidative phosphorylation, nucleotide amino acid epithelial cells GC. Cellular demonstrated donor metabolite betaine could inhibit activity, invasion, migration while activating phosphorylation AMPKα. In conclusion, 1CM-related pathway play significant roles influences warrant further investigation.

Language: Английский

---

Serine Hydroxymethyltransferase Modulates Midgut Physiology in Aedes aegypti Through miRNA Regulation: Insights from Small RNA Sequencing and Gene Expression Analysis

Biomolecules, Journal Year: 2025, Volume and Issue: 15(5), P. 644 - 644

Published: April 30, 2025

Aedes aegypti mosquitoes are critical vectors of arboviruses, responsible for transmitting pathogens that pose significant public health challenges. Serine hydroxymethyltransferase (SHMT), a key enzyme in one-carbon metabolism, plays vital role various biological processes, including DNA synthesis, energy and cell proliferation. Although SHMT is expressed at low levels the midgut aegypti, its silencing has been shown to inhibit blood meal digestion. The precise mechanisms by which regulates physiology remain poorly understood. In this study, we employed small RNA sequencing quantitative PCR identify differentially miRNAs (DEMs) following downregulation. We focused on subset DEMs—miR-2940-5p, miR-2940-3p, miR-2941, miR-306-5p—to explore their potential functions. To further elucidate molecular underlying miRNA response downregulation, analyzed expression genes involved biogenesis pathway. Our results demonstrated several enzymes, Drosha, Dicer1, AGO1, exhibited changes upon silencing. This study provides new insights into through influences functions nutritional metabolism mosquito midgut. By linking activity regulation, our findings highlight pathway modulates physiology, offering foundation future research biology vector control strategies.

Language: Английский

---

Metabolomic Plasma Profile of Chronic Obstructive Pulmonary Disease Patients

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(10), P. 4526 - 4526

Published: May 9, 2025

The analysis of blood metabolites may help identify individuals at risk having COPD and offer insights into its underlying pathophysiology. This study aimed to COPD-related metabolic alterations generate a biological signature potentially useful for screening purposes. Plasma metabolomic profiles from 91 patients controls were obtained using complementary semi-targeted untargeted LC-MS approaches. Univariate identified with significant differences between groups, enrichment highlighted the most affected pathways. Multivariate analysis, including ROC curve assessment machine learning algorithms, was applied assess discriminatory capacity selected metabolites. After adjustment major potential confounders, 56 showed controls. revealed that COPD-associated primarily involved lipid metabolism (especially fatty acids acylcarnitines), followed by amino acid pathways xenobiotics. A panel 10 metabolites, mostly related metabolism, demonstrated high performance (ROC-AUC: 0.916; 90.1% sensitivity 89% specificity). These findings contribute improving strategies better understanding changes. However, our remain exploratory should be interpreted caution, needing further validation mechanistic studies.

Language: Английский

---

Polymer thin film-embedded metal oxide-modified electrochemical paper-based sensor for glycine detection

RSC Advances, Journal Year: 2025, Volume and Issue: 15(20), P. 15630 - 15638

Published: Jan. 1, 2025

A simple electrochemical paper-based sensor (ePAS) using mPEG- b -PLG/CdO quantum dots enabled selective glycine detection. Wax-printed channels and carbon/silver electrodes made this green, reusable, low-cost platform suitable for real samples.

Language: Английский

---

Sex-based differences in in vitro digestibility of milk and oat drink, and powder counterparts

Food Research International, Journal Year: 2025, Volume and Issue: unknown, P. 116610 - 116610

Published: May 1, 2025

Language: Английский

---

Comprehensive analysis of peripheral blood free amino acids in MASLD: the impact of glycine-serine-threonine metabolism

Amino Acids, Journal Year: 2024, Volume and Issue: 57(1)

Published: Dec. 24, 2024

Little is known about how blood free amino acids (FAAs) change in metabolic dysfunction-associated steatotic liver disease (MASLD). This study aims to identify the imbalance of FAAs MASLD and explore its correction as a potential therapeutic target. We analyzed plasma data from 23,036 individuals with steatosis information biobank Japan, 310 patients were enrolled. According diagnostic criteria for (SLD) or cardiometabolic (CC), we divided subjects into five groups: MASLD, dysfunction alcohol-associated (MetALD), CC-SLD-, CC + SLD-, CC-SLD . Twenty compared among these groups pathological information. Among 20 FAAs, levels 16 increased SLD- according number matches items associated insulin resistance (IR). Steatosis enhanced most changes but serine (Ser) threonine (Thr) unaffected. Glycine (Gly), Ser Thr significantly decreased grade. investigated association between imbalances pathogenesis using mouse models. In mice fed high-fat, fructose, cholesterol (FFC) diet, metabolomics RNA sequencing analyses indicated that abnormality Gly, Ser, metabolism was mitochondrial glycolysis via pyruvate. High-Gly, diet ameliorated leptin-deficient mice. Most increase due abnormalities, particularly IR. However, interventions targeting have improve MASLD.

Language: Английский

---

Comprehensive Analysis of Peripheral Blood Free Amino Acids in MASLD: The Impact of Glycine-Serine-Threonine Metabolism

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 22, 2024

Little is known about how blood free amino acids (FAAs) change in metabolic dysfunction-associated steatotic liver disease (MASLD). This study aims to identify the imbalance of FAAs MASLD and explore its correction as a potential therapeutic targets. We analyzed plasma data from 23,036 individuals with steatosis information biobank Japan, 310 patients were enrolled. According diagnostic criteria for (SLD) or cardiometabolic (CC), we divided subjects into five groups: MASLD, dysfunction alcohol-associated (MetALD), CC-SLD-, CC + SLD-, CC-SLD+. Twenty compared among these groups, pathological information. Among 20 FAAs, levels 16 increased SLD- according number matches items associated insulin resistance (IR). Steatosis enhanced most changes but serine (Ser) threonine (Thr) unaffected. Glycine (Gly), Ser Thr significantly decreased grade. investigated association between imbalances pathogenesis using mouse models. In mice fed high-fat, fructose cholesterol (FFC) diet, metabolomics RNA sequencing analyses indicated that abnormality Gly, Ser, metabolism was mitochondrial glycolysis via pyruvate. High-Gly, diet ameliorated leptin-deficient mice. Most increase due abnormalities, particularly IR. However, interventions targeting have improve MASLD.

Language: Английский

---

Multi-omics reveals the beneficial effect of Scytosiphon lomentaria fucoidan on glucose and lipid metabolism disorders via regulation of the gut-liver axis in pregnant mice

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105436 - 105436

Published: Nov. 1, 2024

Language: Английский

---