Clinical accuracy of instrument-based SARS-CoV-2 antigen diagnostic tests: a systematic review and meta-analysis

Virology Journal, Journal Year: 2024, Volume and Issue: 21(1)

Published: April 29, 2024

During the COVID-19 pandemic, antigen diagnostic tests were frequently used for screening, triage, and diagnosis. Novel instrument-based (iAg tests) hold promise of outperforming their instrument-free, visually-read counterparts. Here, we provide a systematic review meta-analysis SARS-CoV-2 iAg tests' clinical accuracy.

Language: Английский

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Clinical accuracy of instrument-based SARS-CoV-2 antigen diagnostic tests: A systematic review and meta-analysis

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 5, 2024

Background During the COVID-19 pandemic, antigen diagnostic tests were frequently used for screening, triage, and diagnosis because they are faster less expensive than molecular tests, despite being sensitive terms of analytical clinical performance. Novel instrument-based (iAg tests) hold promise outperforming their instrument-free, visually-read counterparts. Here, we provide a systematic review meta-analysis SARS-CoV-2 iAg tests’ accuracy. Methods We systematically searched MEDLINE (via PubMed), Web Science, medRxiv, bioRxiv articles published before November 7th, 2022, evaluating accuracy detection. performed random effects to estimate sensitivity specificity QUADAS-2 tool assess study quality risk bias. Results compiled data from 117 studies involving 95,181 individuals assessing 24 commercial tests. The varied in bias but showed high applicability. Of 99 assessed meta-analysis, pooled compared testing paired NP swab sample 76.7% (95% CI 73.5 79.7) 98.4% 98.0 98.7), respectively. Out eight with sufficient test-specific only LumiraDx satisfied WHO's standards. Higher was noted viral load (99.6% [95% 96.8 100] at Ct-level ≤ 20) within first week symptom onset (84.6% 78.2 89.3]), did not differ between conducted as per manufacturer’s instructions those differently, or point-of-care lab-based testing. Conclusion Our reveals that tests' increases lower Ct-values, proxy load, onset, enabling reliable identification most cases. While these characteristics similar instrument-free diagnostics, different types might allow standardized result interpretation curb human error, automated reporting, upscaling test runs additional functions, such indication levels. Depending on setup, can be point care laboratory high-throughput.

Language: Английский

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Current Perspectives on Antigen Testing in Respiratory Infections

The Pediatric Infectious Disease Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 7, 2024

Language: Английский

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