Bio-Based Surfactants and Biosurfactants: An Overview and Main Characteristics

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 863 - 863

Published: Feb. 13, 2025

Natural surfactants are surface-active molecules synthesized from renewable resources (i.e., plants, animals, or microorganisms) and possess properties comparable to conventional surfactants, making them an environmentally friendly potential alternative petrochemical surfactants. Additionally, they exhibit biological such as anti-microbial properties, biodegradability, less toxicity, allowing their use in everyday products with minimal risk human health the environment. Based on mode of production, natural can be classified into first-generation bio-based second-generation biosurfactants, although definition may vary depending author literature. This review offers extensive classification focusing composition, sources, production methods, applications across various industries. Furthermore, main challenges future perspectives discussed.

Language: Английский

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Bacillus lipopeptides as key players in rhizosphere chemical ecology

Trends in Microbiology, Journal Year: 2024, Volume and Issue: 33(1), P. 80 - 95

Published: Aug. 29, 2024

HighlightsCyclic lipopeptides (CLPs) represent unique bacterial natural products with an amazing chemical diversity driving multiple potent bioactivities.More than just lubricants, solubilizers or killing agents, CLPs retain unsuspected array of functions and feature as private tools shared goods produced on demand.Resolving the molecular rules CLP selectivity for a given function requires combined expertise in biology, structural chemistry, biophysics to comprehensively understand their intricate interactions biological membranes.Discovering new should exploit multilevel microbial involve recent advances metagenomics, genome mining, community metabolomicsDeciphering ultimate proximate aspects lipopeptide science highlights relevance ecology.AbstractMicrobial are widely explored therapeutic potential. Understanding underlying evolutionary adaptive forces production remains fundamental question biology. Amphiphilic cyclic (CLPs), prominent category specialized metabolites, show strong antimicrobial activity, particularly against phytopathogens. It is thus assumed that these compounds deployed by soil- rhizosphere-dwelling bacteria weapons competitive environments. Here, we challenge this reductionist perspective present evidence Bacillus mediators ecological interactions. They help communicate, compete, defend predators, cooperate establish mutualistic relationships other (micro)organisms. Additional parallel examples highlighted genera, such Pseudomonas. This broader underscores need further investigation into role shaping strategies key rhizobacterial species.

Language: Английский

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Antimicrobial Activity of Bacillus Cyclic Lipopeptides and Their Role in the Host Adaptive Response to Changes in Environmental Conditions

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 336 - 336

Published: Jan. 2, 2025

Bacillus cyclic lipopeptides (CLP), part of the three main families—surfactins, iturins, and fengycins—are secondary metabolites with a unique chemical structure that includes both peptide lipid components. Being amphiphilic compounds, CLPs exhibit antimicrobial activity in vitro, damaging membranes microorganisms. However, concentrations used vitro are difficult to achieve natural conditions. Therefore, environment, alternative mechanisms action by more likely, such as inducing apoptosis fungal cells, preventing microbial adhesion substrate, promoting death phytopathogens stimulating plant immune responses. In addition, low act signaling molecules Bacillus’s own metabolism, when environmental conditions change, they form an adaptive response host bacterium. Namely, trigger differentiation bacterial population into various specialized cell types: competent flagellated matrix producers, spores. this review, we have summarized current understanding under experimental We also shown relationship between some regulatory pathways involved CLP biosynthesis differentiation, well role determine changes physiological state subpopulations shifts

Language: Английский

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Fungal membrane determinants affecting sensitivity to antifungal cyclic lipopeptides from Bacillus spp.

Fungal Biology, Journal Year: 2024, Volume and Issue: 128(7), P. 2080 - 2088

Published: Aug. 15, 2024

Bacillus spp. produce numerous antimicrobial metabolites. Among these metabolites, cyclic lipopeptides (CLP) including fengycins, iturins, and surfactins are known to have varying antifungal activity against phytopathogenic fungi. The differential activities of CLP been attributed diverse mechanisms action on fungal membranes. However, the precise biochemical determinants driving their modes not conclusively identified. In this study, three plant pathogenic fungi lipopeptide sensitivities, Alternaria solani, Cladosporium cucumerinum, Fusarium sambucinum, were studied determine how cell membrane lipid compositions may confer sensitivity and/or tolerance fengycin, iturin, surfactin. Results indicated that all correlated with lower ergosterol content elevated phospholipid fatty acid unsaturation. Fungal surfactin was also notably different than fengycin as influenced more by phosphatidylethanolamine amounts, higher levels phosphatidylinositol, less acyl chain length. from study provide insight into composition A. F. C. cucumerinum specific characteristics influencing effectiveness Understanding should enable accurate prediction sensitivity-tolerance outcomes for other species exposed important CLP.

Language: Английский

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Ultrasonic preparation, structural characterization and functional enhancement of thyme essential oil-loaded microcapsules based on mannosylerythritol lipid-A

Ultrasonics Sonochemistry, Journal Year: 2025, Volume and Issue: 114, P. 107265 - 107265

Published: Feb. 9, 2025

Mannosylerythritol lipid-A (MEL-A) is a kind of novel biosurfactant and has great potential to apply into food pharmaceutical field with its outstanding physicochemical biological property. In this study, Thyme essential oil (TEO) microcapsules based on MEL-A were prepared through ultrasonic emulsification characterized by size, morphology, structure, antioxidant antibacterial activity. The results showed the optimal preparation condition was duration 15 min power intensities 400 W/cm2 ultrasound treatment, improving solubility applicability TEO. Further experiment explored properties activity TEO microcapsules, measuring particle size 276.19 ± 1.72 nm good dispersibility. FT-IR, X-ray, TEM confirmed successful encapsulation within microcapsules. Meanwhile, assayed 7 % exhibited better properties, while those containing 13 performance. conclusion, obvious structural stability functional enhancement in TEO-loaded indicating that applications preservation machinery sterilization are numerous.

Language: Английский

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Eco-friendly coating engineered with antimicrobial lipopeptides maintains freshness and induces genes expression in anthocyanin biosynthesis of blueberry

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141590 - 141590

Published: March 1, 2025

Language: Английский

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Biomimetic surfactants for tunable interfacial properties in drug delivery, biomedical coatings and tissue engineering

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: 677, P. 125658 - 125658

Published: April 30, 2025

Language: Английский

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Phospholipid membrane permeabilization and leakage of cell content by surfactin-C15 from novel B. Subtilis B-11 strain: a computational and experimental analysis

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: June 5, 2025

Surfactin-C₁₅ produced by novel Bacillus Subtilis B-11 strain has potential to inhibit phytopathogens permeabilizing their phospholipid cell membranes at the water/bilayer interface. This permeabilization leads disintegration of membranes, thus inhibiting growth, replication, and pathogenicity phytopathogens. Model dipalmitoyl phosphocholine (DPPC) vesicles for pathogenic were prepared liposomal assays used as representatives bilayer membrane. Results show that hydrophobic fatty acid tail surfactin-C₁₅ binds with acyl chains DPPC membrane rather than hydrophilic head groups which tilt these chains, causing lipid headgroups reorient forming pores in AFM results structural disorderness increases nanoscale, specifically within range 0 3 nm. The fluorescence intensity encapsulated carboxyfluorescein probe a concentration-dependent manner 25 µM, 50 75 measured constant concentration 10 showing an emission increase from 200 800 Heat flow decreases DPPC: (100:0) pretransition temperature T_m 42.2 ± 0.1 (T_onset 40.9 0.1) (10:90) 39.2 36.9 0.1). An cholesterol causes size 240 nm 285 These confirm larger exhibit higher interfacial activity compared smaller vesicles, due greater surface area exposed membrane-water vesicle content is likely cholesterol’s ability modulate fluidity packing, resulting altered morphology. provide more extensive contact molecules interface, facilitating stronger interactions disrupt integrity enhance antimicrobial efficacy. study suggests could be exploited developing major biocontrol strategies agriculture field.

Language: Английский

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Biosurfactants and bioamphiphiles, survey, perspectives and applicative potential from a colloid science point of view

Current Opinion in Colloid & Interface Science, Journal Year: 2024, Volume and Issue: unknown, P. 101891 - 101891

Published: Dec. 1, 2024

Language: Английский

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Deciphering the mechanisms involved in reduced sensitivity to azoles and fengycin lipopeptide in Venturia inaequalis

Microbiological Research, Journal Year: 2024, Volume and Issue: 286, P. 127816 - 127816

Published: June 27, 2024

Apple scab, caused by the hemibiotrophic fungus Venturia inaequalis, is currently most common and damaging disease in apple orchards. Two strains of V. inaequalis (S755 Rs552) with different sensitivities to azole fungicides bacterial metabolite fengycin were compared determine mechanisms responsible for these differences. Antifungal activity tests showed that Rs552 had reduced sensitivity tebuconazole tetraconazole, as well alone or a binary mixture other lipopeptides (iturin A, pumilacidin, lichenysin). S755 was highly sensitive fengycin, whose close tebuconazole. Unlike from iturin family (mycosubtilin, A) similar on both strains, while those surfactin (lichenysin, pumilacidin) not active, except mixtures fengycin. The varies according their structure. Analyses difference azoles (which target CYP51 enzyme involved ergosterol biosynthesis pathway) linked (i) constitutive increased expression Cyp51A gene insertions upstream region (ii) greater efflux membrane pumps involvement ABC transporters. Microscopic observations revealed known interact plasma membranes, induced morphological cytological changes cells strains. Sterol phospholipid analyses higher level ergosta-7,22-dien-3-ol lower PI(C16:0/C18:1) S755. These differences could therefore influence composition explain differential However, antifungal activities mycosubtilin A two indirectly indicate sterols are probably resistance mechanism. This leads conclusion susceptibility studied.

Language: Английский

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