Cytotoxicity Evaluation of Microbial Sophorolipids and Glucolipids using Normal Human Dermal Fibroblasts (NHDF) in vitro DOI Creative Commons

Sergio Oliveira Formoso,

Vincent Chaleix, Niki Baccile

et al.

Toxicology Reports, Journal Year: 2024, Volume and Issue: 14, P. 101862 - 101862

Published: Dec. 11, 2024

Fibroblasts are considered a key player in the wound healing process. Although this cellular family is constituted by several distinct subtypes, dermal fibroblasts crucial thanks to their ability secrete pro-regenerative growth factors, extracellular matrix (ECM) proteins and immune anti-inflammatory role. Sophorolipids (SL), sophorosides (SS) glucolipids (G), mono-unsaturated (C18:1) or saturated (C18:0), glycolipids derived from microbial fermentation of wild type engineered yeast Starmerella bombicola, constitute novel sustainable class bio-based chemicals with interesting physicochemical characteristics, which allow them form soft diverse structures hydrogels vesicles, micelles complex coacervates potential interest skin regeneration applications. In study, we first tested cytocompatibility broad set molecules on normal human (NHDF). Our results show that, up an upper threshold (0.1 % w/v), (SL-C18:1, G-C18:1, SSbola-C18:1, SL-C18:0 G-C18:0) under study were able sustain cell growth. Furthermore, selected least cytotoxic SL-C18:0) promote measuring gene expression markers (i.e. collagen, elastin, transforming factor β, fibroblast …) using qPCR. Unfortunately, none these modulated involved tissue repair. However, aims encourage community test for high-end biomedical Biosurfactants prepared natural amphiphiles growing importance, goal replacing synthetic surfactants commercial formulations. cytotoxicity profile still poorly known, especially new like single-glucose lipids bolaform sophorolipids. This wants contribute all those applications, could be developed biosurfactants contact (cosmetics, healing). We five structurally-related (C18:1 C18:0 sophorolipids, C18:1 lipids, di-sophoroside) against (NHDF) evaluate metabolic activity toxic among them. To best our knowledge, molecules, general, was never NHDF.

Language: Английский

Synthetic approaches of carbohydrate based self-assembling systems DOI Creative Commons
Guijun Wang, Anji Chen, Pramod Aryal

et al.

Organic & Biomolecular Chemistry, Journal Year: 2024, Volume and Issue: 22(27), P. 5470 - 5510

Published: Jan. 1, 2024

The synthesis of carbohydrate-derived self-assembling systems and the properties resulting stimulus-responsive materials via bottom-up approaches are reviewed.

Language: Английский

Citations

3
Biosurfactants and bioamphiphiles, survey, perspectives and applicative potential from a colloid science point of view DOI
Niki Baccile, Jochen Kleinen

Current Opinion in Colloid & Interface Science, Journal Year: 2024, Volume and Issue: unknown, P. 101891 - 101891

Published: Dec. 1, 2024

Language: Английский

Citations

2
Cytotoxicity Evaluation of Microbial Sophorolipids and Glucolipids using Normal Human Dermal Fibroblasts (NHDF) in vitro DOI Creative Commons

Sergio Oliveira Formoso,

Vincent Chaleix, Niki Baccile

et al.

Toxicology Reports, Journal Year: 2024, Volume and Issue: 14, P. 101862 - 101862

Published: Dec. 11, 2024

Fibroblasts are considered a key player in the wound healing process. Although this cellular family is constituted by several distinct subtypes, dermal fibroblasts crucial thanks to their ability secrete pro-regenerative growth factors, extracellular matrix (ECM) proteins and immune anti-inflammatory role. Sophorolipids (SL), sophorosides (SS) glucolipids (G), mono-unsaturated (C18:1) or saturated (C18:0), glycolipids derived from microbial fermentation of wild type engineered yeast Starmerella bombicola, constitute novel sustainable class bio-based chemicals with interesting physicochemical characteristics, which allow them form soft diverse structures hydrogels vesicles, micelles complex coacervates potential interest skin regeneration applications. In study, we first tested cytocompatibility broad set molecules on normal human (NHDF). Our results show that, up an upper threshold (0.1 % w/v), (SL-C18:1, G-C18:1, SSbola-C18:1, SL-C18:0 G-C18:0) under study were able sustain cell growth. Furthermore, selected least cytotoxic SL-C18:0) promote measuring gene expression markers (i.e. collagen, elastin, transforming factor β, fibroblast …) using qPCR. Unfortunately, none these modulated involved tissue repair. However, aims encourage community test for high-end biomedical Biosurfactants prepared natural amphiphiles growing importance, goal replacing synthetic surfactants commercial formulations. cytotoxicity profile still poorly known, especially new like single-glucose lipids bolaform sophorolipids. This wants contribute all those applications, could be developed biosurfactants contact (cosmetics, healing). We five structurally-related (C18:1 C18:0 sophorolipids, C18:1 lipids, di-sophoroside) against (NHDF) evaluate metabolic activity toxic among them. To best our knowledge, molecules, general, was never NHDF.

Language: Английский

Citations

0