Chlamydomonas FBB18 is a ubiquitin-like protein essential for the cytoplasmic preassembly of various ciliary dyneins

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(12)

Published: March 19, 2025

Motile cilia are organelles found on many eukaryotic cells that play critical roles in development and fertility. Human CFAP298 has been implicated the transport/assembly of ciliary dyneins, defects this protein cause primary dyskinesia. However, neither exact function nor structure have elucidated. Here, we took advantage Chlamydomonas, a ciliated alga, to study FBB18, an ortholog CFAP298. Multiple dyneins were greatly reduced Chlamydomonas fbb18 mutants. In addition, both stability dynein heavy chains (HCs) association between HCs intermediate/light (IC/LCs) cytoplasm, strongly suggesting FBB18 functions cytoplasmic assembly (the so-called "preassembly") complexes from HC/IC/LCs. Furthermore, X-ray crystallography revealed forms bilobed with globular domains at ends molecule, connected by α-helical bundle. Unexpectedly, one domain shows high similarity ubiquitin, small for modification variety complexes, ubiquitin-like is indispensable molecular FBB18. Our results demonstrate specialized member family, plays role preassembly, most likely mediating diverse interactions HCs, chaperone(s), other preassembly factor(s) using as well regions, facilitating proper folding HCs.

Language: Английский

Editorial Overview: Cilia and Endocrinology

Current Opinion in Endocrine and Metabolic Research, Journal Year: 2024, Volume and Issue: unknown, P. 100558 - 100558

Published: Oct. 1, 2024

Language: Английский