Synthetic biology and engineering,
Journal Year:
2023,
Volume and Issue:
1(1), P. 1 - 9
Published: Jan. 1, 2023
Hydroxybenzoic
acids
(HBAs),
including
4-HBA,
3-HBA,
and
2-HBA,
are
valuable
platform
chemicals
for
production
of
commodity
materials
fine
chemicals.
Herein,
we
employed
metabolic
engineering
techniques
to
enhance
the
these
HBAs
in
Corynebacterium
glutamicum
ATCC
13032.
Our
approach
augmented
shikimate
pathway
eliminated
genes
associated
with
HBA
degradation,
particularly
phenol
2-monooxygenase
encoded
by
cg2966.
Increased
titers
3-HBA
4-HBA
were
achieved
via
selection
suitable
promoters
3-hydroxybenzoate
synthase
chorismate
pyruvate
lyase.
A
tac-M1
promoter
was
lyase
expression
8.3
g/L
achieved.
Efficient
2-HBA
enabled
maintaining
a
balanced
isochorismate
Consequently,
strains
KSD5-tacM1-H
KSD5-J2-PE
exhibited
levels
19.2
12.9
respectively,
using
1
L
jar
fermenter
containing
80
glucose.
Therefore,
this
engineered
strain
holds
significant
potential
other
products
derived
from
chorismate.
iScience,
Journal Year:
2023,
Volume and Issue:
26(8), P. 107255 - 107255
Published: July 3, 2023
The
development
of
biohydrogen
as
an
alternative
energy
source
has
had
great
economic
and
environmental
benefits.
Hydrogen
production
from
microalgae
is
considered
a
clean
sustainable
method
that
can
both
alleviate
fuel
shortages
recycle
waste.
Although
algal
hydrogen
low
consumption
requires
only
simple
pretreatment,
it
not
been
commercialized
because
product
yields.
To
increase
microalgal
several
technologies
have
developed,
although
they
struggle
with
the
oxygen
sensitivity
hydrogenases
responsible
for
complexity
metabolic
network.
In
this
review,
genetic
engineering
studies
on
enhancing
are
discussed,
feasibility
future
direction
commercialization
also
proposed.
Bioresource Technology,
Journal Year:
2023,
Volume and Issue:
381, P. 129147 - 129147
Published: May 9, 2023
Microbial
production
of
medium
chain
length
fatty
acids
(MCFAs)
from
renewable
resources
is
becoming
increasingly
important
in
establishing
a
sustainable
and
clean
chemical
industry.
This
review
comprehensively
summarizes
current
advances
microbial
MCFA
resources.
Detailed
information
provided
on
two
major
pathways
using
various
other
auxiliary
supporting
to
help
understand
the
fundamentals
bio-based
production.
In
addition,
conventional
well-studied
producers
are
classified
into
categories,
natural
synthetic
producers,
their
characteristics
outlined.
Moreover,
engineering
strategies
employed
achieve
highest
MCFAs
up
date
showcased
together
with
key
enzymes
suggested
for
overproduction.
Finally,
future
challenges
perspectives
discussed
towards
more
efficient
Critical Reviews in Biotechnology,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 20
Published: May 30, 2024
Second
generation
biorefineries
play
an
important
role
in
the
production
of
renewable
energy
and
fuels,
utilizing
forest
agro-industrial
residues
by-products
as
raw
materials.
The
integration
novel
bioproducts,
such
as:
xylitol,
β-carotene,
xylooligosaccharides,
biopigments
into
biorefinery's
portfolio
can
offer
economic
benefits
valorization
lignocellulosic
materials,
particularly
cellulosic
hemicellulosic
fractions.
Fungal
biopigments,
known
for
their
additional
antioxidant
antimicrobial
properties,
are
appealing
to
consumers
have
applications
various
industrial
sectors,
including
food
pharmaceuticals.
use
materials
carbon
nutrient
sources
growth
medium
helps
reduce
costs,
increasing
competitiveness
fungal
market.
In
addition,
implementation
biopigment
allows
utilization
underutilized
fractions,
hemicellulose,
value-added
bioproducts.
This
study
deals
with
potential
second
order
diversify
produced
biomolecules
together
generation.
A
comprehensive
critical
review
recent
literature
on
this
topic
has
been
conducted,
covering
major
possible
general
aspects
biorefineries,
incorporation
biorefineries.
Industrial Biotechnology,
Journal Year:
2023,
Volume and Issue:
19(3), P. 125 - 137
Published: June 1, 2023
As
concerns
over
climate
crisis
and
environmental
issues
continue
to
mount,
there
is
an
urgent
need
transition
towards
a
more
sustainable
society.
One
key
aspect
of
this
establishing
systems
for
production
industrially
important
chemicals
from
fossil
feedstocks
renewable
biomass.
Succinic
acid
(SA)
recognized
as
one
the
most
platform
due
its
potential
in
numerous
industrial
applications.
Consequently,
extensive
efforts
have
been
made
develop
metabolically
engineered
microbial
strains
capable
producing
SA
with
high
efficiency.
In
paper,
current
status
reviewed
accompanying
table
summarizing
titer,
yield,
productivity
achieved
by
various
microorganisms.
addition,
paper
discusses
downstream
processes
purification
biobased
showcases
several
derivatives
interest.
Finally,
future
perspectives
on
successful
industrialization
are
discussed.
Proceedings of the National Academy of Sciences,
Journal Year:
2024,
Volume and Issue:
121(37)
Published: Sept. 6, 2024
Succinic
acid
(SA),
a
dicarboxylic
of
industrial
importance,
can
be
efficiently
produced
by
metabolically
engineered
Mannheimia
succiniciproducens
.
Although
the
importance
magnesium
(Mg
2+
)
ion
on
SA
production
has
been
evident
from
our
previous
studies,
role
Mg
remains
largely
unexplored.
In
this
study,
we
investigated
impact
and
developed
hyper-SA
producing
strain
M.
reconstructing
transport
system.
To
achieve
this,
optimal
alkaline
neutralizer
comprising
was
physiological
effect
analyzed.
Subsequently,
system
reconstructed
introducing
an
efficient
transporter
Salmonella
enterica
A
high-inoculum
fed-batch
fermentation
final
152.23
±
0.99
g/L
SA,
with
maximum
productivity
39.64
0.69
g/L/h.
These
findings
highlight
ions
transportation
optimization
in
succinic
