Hydroxybenzoic Acid Production Using Metabolically Engineered Corynebacterium glutamicum DOI Creative Commons

Misa Doke,

Mayumi Kishida,

Yuuki Hirata

et al.

Synthetic biology and engineering, Journal Year: 2023, Volume and Issue: 1(1), P. 1 - 9

Published: Jan. 1, 2023

Hydroxybenzoic acids (HBAs), including 4-HBA, 3-HBA, and 2-HBA, are valuable platform chemicals for production of commodity materials fine chemicals. Herein, we employed metabolic engineering techniques to enhance the these HBAs in Corynebacterium glutamicum ATCC 13032. Our approach augmented shikimate pathway eliminated genes associated with HBA degradation, particularly phenol 2-monooxygenase encoded by cg2966. Increased titers 3-HBA 4-HBA were achieved via selection suitable promoters 3-hydroxybenzoate synthase chorismate pyruvate lyase. A tac-M1 promoter was lyase expression 8.3 g/L achieved. Efficient 2-HBA enabled maintaining a balanced isochorismate Consequently, strains KSD5-tacM1-H KSD5-J2-PE exhibited levels 19.2 12.9 respectively, using 1 L jar fermenter containing 80 glucose. Therefore, this engineered strain holds significant potential other products derived from chorismate.

Language: Английский

Improving microbial bioproduction under low-oxygen conditions DOI

Shawn Kulakowski,

Deepanwita Banerjee, Corinne D. Scown

et al.

Current Opinion in Biotechnology, Journal Year: 2023, Volume and Issue: 84, P. 103016 - 103016

Published: Nov. 3, 2023

Language: Английский

Citations

3
Tuning Fatty Acid Profile and Yield in Pichia pastoris DOI Creative Commons

Simon Kobalter,

Alena Voit,

Myria Bekerle‐Bogner

et al.

Bioengineering, Journal Year: 2023, Volume and Issue: 10(12), P. 1412 - 1412

Published: Dec. 12, 2023

Fatty acids have been supplied for diverse non-food, industrial applications from plant oils and animal fats many decades. Due to the massively increasing world population demanding a nutritious diet thrive provide feedstocks production lines in sustainable way, i.e., independent food supply chains, alternative fatty acid sources gained importance. Carbohydrate-rich side-streams of agricultural production, e.g., molasses, lignocellulosic waste, glycerol biodiesel even CO2, are considered employed as carbon fermentative accumulation selected microbial hosts. While certain species readily accumulated native metabolic routes, other scarce, host strains need be metabolically engineered their high-level production. We report engineering Pichia pastoris produce palmitoleic glucose discuss beneficial detrimental steps detail. secretion was achieved through deletion acyl-CoA synthetases overexpression truncated E. coli thioesterase 'TesA. The best secreted >1 g/L free into culture medium. Additionally, introduction C16-specific ∆9-desaturases synthases, coupled with improved cultivation conditions, increased content 5.5% 22%.

Language: Английский

Citations

2
Ductile Copolyesters Prepared Using Succinic Acid, 1,4-Butanediol, and Bis(2-hydroxyethyl) Terephthalate with Minimizing Generation of Tetrahydrofuran DOI Open Access
Sang Uk Park,

Hyeon Jeong Seo,

Yeong Hyun Seo

et al.

Polymers, Journal Year: 2024, Volume and Issue: 16(4), P. 519 - 519

Published: Feb. 14, 2024

Poly(1,4-butylene succinate) (PBS) is a promising sustainable and biodegradable synthetic polyester. In this study, we synthesized PBS-based copolyesters by incorporating 5–20 mol% of –O2CC6H4CO2– –OCH2CH2O– units through the polycondensation succinic acid (SA) with 1,4-butanediol (BD) bis(2-hydroxyethyl) terephthalate (BHET). Two different catalysts, H3PO4 conventional catalyst (nBuO)4Ti, were used comparatively in synthesis process. The produced using former treated M(2-ethylhexanoate)2 (M = Mg, Zn, Mn) to connect chains ionic interactions between M2+ ions either –CH2OP(O)(OH)O− or (–CH2O)2P(O)O− groups. By BHET (i.e., –OCH2CH2O–), resulting exhibited improved ductile properties enhanced elongation at break, albeit reduced tensile strength. prepared H3PO4/M(2-ethylhexanoate)2 displayed less random distribution units, leading faster crystallization rate, higher Tm value, yield strength compared those (nBuO)4Ti same amount BHET. Furthermore, they substantial shear-thinning behavior their rheological due presence long-chain branches (–CH2O)3P=O units. Unfortunately, H3PO4/M(2-ethylhexanoate)2, hence containing M2+, –CH2OP(O)(OH)O−, groups, did not exhibit biodegradability under ambient soil conditions.

Language: Английский

Citations

0
Use of antioxidants to extend the storage of lyophilized cell-free synthesis system DOI

Kyu Jae Kim,

So Jeong Lee, Dong‐Myung Kim

et al.

Biotechnology and Bioprocess Engineering, Journal Year: 2024, Volume and Issue: 29(2), P. 263 - 269

Published: March 13, 2024

Language: Английский

Citations

0
Hydroxybenzoic Acid Production Using Metabolically Engineered Corynebacterium glutamicum DOI Creative Commons

Misa Doke,

Mayumi Kishida,

Yuuki Hirata

et al.

Synthetic biology and engineering, Journal Year: 2023, Volume and Issue: 1(1), P. 1 - 9

Published: Jan. 1, 2023

Hydroxybenzoic acids (HBAs), including 4-HBA, 3-HBA, and 2-HBA, are valuable platform chemicals for production of commodity materials fine chemicals. Herein, we employed metabolic engineering techniques to enhance the these HBAs in Corynebacterium glutamicum ATCC 13032. Our approach augmented shikimate pathway eliminated genes associated with HBA degradation, particularly phenol 2-monooxygenase encoded by cg2966. Increased titers 3-HBA 4-HBA were achieved via selection suitable promoters 3-hydroxybenzoate synthase chorismate pyruvate lyase. A tac-M1 promoter was lyase expression 8.3 g/L achieved. Efficient 2-HBA enabled maintaining a balanced isochorismate Consequently, strains KSD5-tacM1-H KSD5-J2-PE exhibited levels 19.2 12.9 respectively, using 1 L jar fermenter containing 80 glucose. Therefore, this engineered strain holds significant potential other products derived from chorismate.

Language: Английский

Citations

0