Dendritic Cells Overcome Cre/Lox Induced Gene Deficiency by Siphoning Material From Neighboring Cells Using Intracellular Monitoring—a Novel Mechanism of Antigen Acquisition DOI Creative Commons

Christopher H. Herbst,

Aurélie Bouteau,

Evelin J. Menykő

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 25, 2023

Macrophages and dendritic cells (DCs) in peripheral tissue interact closely with their local microenvironment by scavenging protein nucleic acids released neighboring cells. Material transfer between cell types is necessary for pathogen detection antigen presentation, but thought to be relatively limited scale. Recent reports, however, demonstrate that the quantity of transferred material can quite large when DCs are direct contact live This observation may problematic conditional gene deletion models assume products will remain they produced in. Here, we investigate whether deletions induced widely used Cre/Lox system overcome at level DCs. Of concern, using human Langerin Cre mouse model, find epidermal Langerhans CD11b+CD103+ mesenteric if deleted expressed Surprisingly, also mechanism does not resemble known mechanisms uptake, dependent on extra- intracellular calcium, PI3K, scavenger receptors, mediates a majority We term this novel process

Language: Английский

Dendritic Cells Overcome Cre/Lox Induced Gene Deficiency by Siphoning Material From Neighboring Cells Using Intracellular Monitoring—a Novel Mechanism of Antigen Acquisition DOI Creative Commons

Christopher H. Herbst,

Aurélie Bouteau,

Evelin J. Menykő

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 25, 2023

Macrophages and dendritic cells (DCs) in peripheral tissue interact closely with their local microenvironment by scavenging protein nucleic acids released neighboring cells. Material transfer between cell types is necessary for pathogen detection antigen presentation, but thought to be relatively limited scale. Recent reports, however, demonstrate that the quantity of transferred material can quite large when DCs are direct contact live This observation may problematic conditional gene deletion models assume products will remain they produced in. Here, we investigate whether deletions induced widely used Cre/Lox system overcome at level DCs. Of concern, using human Langerin Cre mouse model, find epidermal Langerhans CD11b+CD103+ mesenteric if deleted expressed Surprisingly, also mechanism does not resemble known mechanisms uptake, dependent on extra- intracellular calcium, PI3K, scavenger receptors, mediates a majority We term this novel process

Language: Английский

Citations

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