An NF‐κB‐regulated cytokine enhances the antiviral resistance of silkworm, Bombyx mori DOI Open Access

Dongmei Wei,

Jinming Liu, Jie Hu

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Insect NF‐κB‐like factor, Relish, is activated by viral infection and induces the production of antiviral proteins. In this study, we performed a transcriptomic analysis BmE cells expressing active form BmRelish (BmRelish act ) identified BmVago‐like as most strongly‐induced secreted‐protein. Expression was specifically triggered Bombyx mori Nucleo Polyhedro Virus (BmNPV) regulated BmSTING‐BmRelish pathway. Incubating fresh culture with supernatant medium or recombinant protein (rBmVago‐like) significantly increased resistance. On contrary, reducing expression Bmvago‐like RNA interference (RNAi) in well silkworm larvae impaired response. Furthermore, constructed transgenic line over‐expressing (BmVago‐like OV found they had markedly lower load higher survival rate after BmNPV compared wild‐type control. Co‐immunoprecipitation assay showed Bmintegrin β1 interacts it involved mediated Finally, level signalling molecules JAK–STAT pathway rBmVago‐like‐treated but decreased RNAi‐treated cells. summary, our research uncovered an inducible response cytokine BmVago‐like, which downstream effector functions cytokine.

Language: Английский

Highly Pathogenic Avian Influenza (HPAI) H5N1 virus in Finland in 2021-2023 – Genetic diversity of the viruses and infection kinetics in human dendritic cells DOI Creative Commons
Eda Altan, Veera Avelin, Kirsi Aaltonen

et al.

Emerging Microbes & Infections, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Highly pathogenic avian influenza (HPAI) H5N1 is known for its virulence and zoonotic potential, infecting birds mammals, thus raising public health concerns. Since 2021 spread among has led to cross-species transmission causing epizootics eventually impacting fur animal farms in Finland 2023. To analyze the infectivity of Finnish isolates human cells, representatives diverse were selected based on genetic differences, host species, year occurrence. The infection kinetics from wild pheasant fox, animals blue fox white mink examined monocyte-derived dendritic cells (moDCs) with isolate as a control. Although (a bird) showed weakly reduced replication viral protein expression compared mammalian isolates, no discernible differences virus moDCs was observed. This study revealed similar all five regardless observed differences. While infections remain rare, poses risk widespread mammals such and, more recently, dairy cattle.

Language: Английский

Citations

0
Radiation exposure induced blood-brain barrier injury via mitochondria-mediated sterile inflammation DOI Open Access
Peng Wang, Jiayue Liu, Min Zhang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Abstract Radiation-induced brain injury (RIBI) is caused by exposure to high doses of ionizing radiation and characterized severe cognitive dysfunction necrosis. The widespread application radiotherapy rapid development deep space exploration have substantially increased the risk RIBI. However, pathogenesis RIBI not fully understood, no effective intervention available. This work described a blood-brain barrier (BBB) microphysiological system (MPS), that allowed explore responses BBB distinct cells exposure. MPS can recapitulate interface structure function in microenvironment, including endothelial cells, astrocytes, pericytes microglia co-cultured under flow condition. Following acute X-ray or γ-ray, characteristic RIBI-associated pathological were observed, compromise, DNA breaks, inhibited cell proliferation, hypertrophy pro-inflammatory cytokine release. Among distinctive types showed highest radiosensitivity as compared other MPS. Intriguingly, γ-ray consistently induced prominent sterile inflammation responses, especially type I interferon response, These mediated radiation-induced mitochondrial release subsequent activation cGAS-STING signaling pathway. Furthermore, we found abrocitinib (JAK1 inhibitor) idebenone (mitochondrial protectant) attenuate ameliorate injury. findings revealed involvement mitochondria-mediated pathogenesis, identifying mitochondria potential target for new radioprotective measures.

Language: Английский

Citations

0
Origin and Function of Anti-Interferon Type I Viral Proteins DOI Creative Commons
Marta Acchioni, Chiara Acchioni, John Hiscott

et al.

Virology, Journal Year: 2025, Volume and Issue: 605, P. 110456 - 110456

Published: Feb. 20, 2025

Language: Английский

Citations

0
Molecular mechanisms in DYT-PRKRA: pathways regulated by PKR activator protein PACT DOI Creative Commons

Tricia A. Simon,

Rekha C. Patel

Dystonia, Journal Year: 2025, Volume and Issue: 4

Published: March 13, 2025

Dystonia- PRKRA (DYT- ), previously termed dystonia 16 (DYT16), is a movement disorder which currently has very limited treatments available and no cure. To develop effective therapeutic options, it essential to characterize the underlying pathophysiology identify potential drug targets. This review summarizes recent studies that shed light on molecular mechanisms involved in DYT- pathogenesis. gene encodes for protein PACT (Protein Activator of Protein Kinase R) individuals with mutations early-onset generalized dystonia. While precise linking neuronal etiology remain incompletely understood, research indicates such cause dysregulation signaling pathways cellular stress response as well production antiviral cytokines interferons (IFNs). focuses effect known functions PACT.

Language: Английский

Citations

0
NF-κB signaling directs a program of transient amplifications at innate immune response genes DOI Creative Commons
Michael P. Ludwig, Jason R. Wilson, Matthew D. Galbraith

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

The cellular response to pathogens involves an intricate directed by key innate immune signaling pathways which is characterized cell-to-cell heterogeneity. How this heterogeneity established and regulated remains unclear. We describe a program of transient site-specific gains (TSSG) producing extrachromosomal DNA (ecDNA) immune-related genes in signaling. Activation NF-κB drives TSSG the interferon receptor gene cluster through inducible recruitment transcription factor RelA pre-replication complex member MCM2 epigenetically control element. Targeted or p300 are sufficient induce formation. specify for at least six as many 179 regions enriched genes. Identification reveals production ecDNA mechanism host response.

Language: Английский

Citations

0
Radiation Exposure Induced Blood–Brain Barrier Injury via Mitochondria‐Mediated Sterile Inflammation DOI Creative Commons
Peng Wang, Jiayue Liu, Min Zhang

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 28, 2025

Abstract Radiation‐induced brain injury (RIBI) is caused by exposure to high doses of ionizing radiation and characterized severe cognitive dysfunction necrosis. However, the pathogenesis RIBI not fully understood, no effective intervention available. This work describes a blood–brain barrier (BBB) microphysiological system (MPS), that allowed explore responses BBB distinct cells exposure. Following acute X‐ray or γ‐ray, characteristic RIBI‐associated pathological are observed, including compromise, DNA breaks, inhibited cell proliferation, hypertrophy, proinflammatory cytokine release. Among distinctive types cells, endothelial show highest radiosensitivity as compared other in MPS. Intriguingly, γ‐ray consistently induce prominent sterile inflammation responses, especially type I interferon response, These mediated radiation‐induced mitochondrial release subsequent activation cGAS‐STING signaling pathway. Furthermore, it found abrocitinib (JAK1 inhibitor) idebenone (mitochondrial protectant) can attenuate ameliorate injuries findings reveal involvement mitochondria‐mediated pathogenesis, identifying mitochondria potential target for new radioprotective measures.

Language: Английский

Citations

0
A new antibacterial with anti-inflammatory properties promotes wound healing through inhibiting cGAS/STING/NF-κB/IRF3 pathway DOI

Zongze Yao,

Miaoqing Xiang,

Yang Yang

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 143, P. 113303 - 113303

Published: Oct. 3, 2024

Language: Английский

Citations

2
UL56 Is Essential for Herpes Simplex Virus-1 Virulence In Vivo but Is Dispensable for Induction of Host-Protective Immunity DOI Creative Commons
Nopprarat Tongmuang,

Meera Krishnan,

Viv Connor

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(8), P. 837 - 837

Published: July 25, 2024

Herpes simplex virus-1 (HSV-1) is common and can cause significant disease in humans. Unfortunately, efforts to develop effective vaccines against HSV-1 have so far failed. A detailed understanding of how the virus infects its host mounts potent immune responses may inform new vaccine approaches. Here, using a zosteriform mouse model, we examined gene

Language: Английский

Citations

1
Nuclear factor kappa-B cell (NF-κB), interferon regulatory Factor, and glucocorticoid receptor pathway activation in major depressive Disorder: The role of cytomegalovirus infection DOI
Jonathan Savitz, Brett A. McKinney, Timothy B. Meier

et al.

Brain Behavior and Immunity, Journal Year: 2024, Volume and Issue: 123, P. 1052 - 1060

Published: Nov. 10, 2024

Language: Английский

Citations

1
An NF‐κB‐regulated cytokine enhances the antiviral resistance of silkworm, Bombyx mori DOI Open Access

Dongmei Wei,

Jinming Liu, Jie Hu

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Insect NF‐κB‐like factor, Relish, is activated by viral infection and induces the production of antiviral proteins. In this study, we performed a transcriptomic analysis BmE cells expressing active form BmRelish (BmRelish act ) identified BmVago‐like as most strongly‐induced secreted‐protein. Expression was specifically triggered Bombyx mori Nucleo Polyhedro Virus (BmNPV) regulated BmSTING‐BmRelish pathway. Incubating fresh culture with supernatant medium or recombinant protein (rBmVago‐like) significantly increased resistance. On contrary, reducing expression Bmvago‐like RNA interference (RNAi) in well silkworm larvae impaired response. Furthermore, constructed transgenic line over‐expressing (BmVago‐like OV found they had markedly lower load higher survival rate after BmNPV compared wild‐type control. Co‐immunoprecipitation assay showed Bmintegrin β1 interacts it involved mediated Finally, level signalling molecules JAK–STAT pathway rBmVago‐like‐treated but decreased RNAi‐treated cells. summary, our research uncovered an inducible response cytokine BmVago‐like, which downstream effector functions cytokine.

Language: Английский

Citations

1