Glia-specific expression of neuropeptide receptor Lgr4 regulates development and adult physiology in Drosophila DOI Creative Commons
Seung Gee Lee, Jasdeep Kaur, Hongyu Miao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Oct. 7, 2022

ABSTRACT Similar to the human brain, Drosophila glia may well be divided into several subtypes that each carry out specific functions. Glial GPCRs plays key roles in crosstalk between neurons and glia. Lgr4 (dLgr4 ) is a relaxin receptor homolog involved angiogenesis, cardiovascular regulation, collagen remodeling, wound healing. Recent study suggests ilp7 might ligand for regulates escape behavior of larvae. Here we demonstrate expression glial cells, not neurons, necessary early development, adult behavior, lifespan. Reducing level but disrupts although knocking down other LGR family members has no impact. Adult-specific knockdown reduce locomotion, male reproductive success, animal longevity. The investigation how contributes this behavioral alteration will increase our understanding insulin signaling via selectively modulates neuronal activity behavior.

Language: Английский

Paying the brain's energy bill DOI Creative Commons
Zahid Padamsey, Nathalie L. Rochefort

Current Opinion in Neurobiology, Journal Year: 2022, Volume and Issue: 78, P. 102668 - 102668

Published: Dec. 24, 2022

How have animals managed to maintain metabolically expensive brains given the volatile and fleeting availability of calories in natural world? Here we review studies support three strategies that involve: 1) a reallocation energy from peripheral tissues functions cover costs brain, 2) an implementation energy-efficient neural coding, enabling brain operate at reduced costs, 3) efficient use costly resources during food scarcity. Collectively, these reveal heterogeneous set energy-saving mechanisms make energy-costly fit for survival.

Language: Английский

Citations

54
Ca2+ excitability of glia to neuromodulator octopamine in Drosophila living brain is greater than that of neurons DOI Creative Commons

Urška Černe,

Anemari Horvat,

Ena Sanjković

et al.

Acta Physiologica, Journal Year: 2025, Volume and Issue: 241(2)

Published: Jan. 13, 2025

Octopamine in the Drosophila brain has a neuromodulatory role similar to that of noradrenaline mammals. After release from Tdc2 neurons, octopamine/tyramine may trigger intracellular Ca We expressed Octopamine-stimulated In optic lobes, astrocytes, not appear be sole responders low concentration octopamine signals, and therefore likely drive synaptic plasticity visual processing. Given interconnectivity lobes with other regions, octopaminergic signals acting through lobe astrocytes also influence higher-order functions including learning memory.

Language: Английский

Citations

1
Mosquito Cell Atlas: A single-nucleus transcriptomic atlas of the adultAedes aegyptimosquito DOI Creative Commons
Olivia V. Goldman,

Alexandra E. DeFoe,

Yanyan Qi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

The female mosquito's remarkable ability to hunt humans and transmit pathogens relies on her unique biology. Here, we present the Mosquito Cell Atlas (MCA), a comprehensive single-nucleus RNA sequencing dataset of more than 367,000 nuclei from 19 dissected tissues adult male Aedes aegypti , providing cellular-level resolution mosquito We identify novel cell types expand our understanding sensory neuron organization chemoreceptors all tissues. Our analysis uncovers male-specific cells sexually dimorphic gene expression in antenna brain. In mosquitoes, find that glial brain, rather neurons, undergo most extensive transcriptional changes following blood feeding. findings provide insights into cellular basis behavior sexual dimorphism. MCA aims serve as resource for vector biology community, enabling systematic investigation cell-type specific across

Language: Английский

Citations

1
In vivo autofluorescence lifetime imaging of the Drosophila brain captures metabolic shifts associated with memory formation DOI Open Access

Philémon Roussel,

Mingyi Zhou, Chiara Stringari

et al.

Published: March 31, 2025

Neuronal energy regulation is increasingly recognized as a critical factor underlying brain functions and their pathological alterations, yet the metabolic dynamics that accompany cognitive processes remain poorly understood. As label-free minimally invasive technique, fluorescence lifetime imaging (FLIM) of coenzymes NADH NADPH (collectively referred to NAD(P)H) offers possibility resolve cellular profiles with high spatial precision. However, NAD(P)H FLIM’s capacity detect subtle changes in neuronal metabolism associated cognition has not been demonstrated. In this study, we applied FLIM map Drosophila neurons vivo across multiple scales, focusing on primary centers for associative memory: mushroom bodies (MBs). At broad scale, obtained an overview signatures main tissue identified marked difference between neuropil cortex areas. finer our findings revealed notable heterogeneity basal distinct MB neuron subtypes. Measurements performed after olfactory learning also uncovered subtype-specific shift memory formation, demonstrating utility detecting physiology-driven linked function. These results establish promising framework studying cerebral vivo.

Language: Английский

Citations

1
Drosophila glial system: an approach towards understanding molecular complexity of neurodegenerative diseases DOI

Shweta Shweta,

Khushboo Sharma,

Mallikarjun Shakarad

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: Nov. 12, 2024

Language: Английский

Citations

4
Pyruvate kinase deficiency links metabolic perturbations to neurodegeneration and axonal protection DOI Creative Commons
Thomas J. Waller, Catherine A. Collins, Monica Dus

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 5, 2025

Neurons rely on tightly regulated metabolic networks to sustain their high-energy demands, particularly through the coupling of glycolysis and oxidative phosphorylation. Here, we investigate role pyruvate kinase (PyK), a key glycolytic enzyme, in maintaining axonal synaptic integrity Drosophila melanogaster neuromuscular system. Using genetic deficiencies PyK, show that disrupting induces progressive degeneration severe locomotor deficits. These effects require conserved dual leucine zipper (DLK), Jun N-terminal (JNK), activator protein 1 (AP-1) Fos transcription factor damage signaling pathway SARM1 NADase driver degeneration. As both DLK regulate injured axons (Wallerian degeneration), probed effect PyK loss this process. Consistent with idea shifts may influence neuronal resilience context-dependent ways, find pyk knockdown delays Wallerian following nerve injury, suggesting reducing flux can promote axon survival under stress conditions. This protective is partially blocked by fully abolished overexpression. Together, our findings help bridge metabolism neurodegenerative demonstrating perturbations causally activate response pathways dictate balance between protection depending system's state. results provide mechanistic framework for understanding contributions neurodegeneration highlight potential as target therapeutic strategies.

Language: Английский

Citations

0
Glia: The Cellular Glue that binds Circadian Rhythms and Sleep DOI Creative Commons

Catarina Cavalhas-Almeida,

Amita Sehgal

SLEEP, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Glia are increasingly appreciated as serving an important function in the control of sleep and circadian rhythms. Glial cells Drosophila mammals regulate daily rhythms locomotor activity well homeostatic rebound following deprivation. In addition, they contribute to proposed functions sleep, with different mapping varied glial subtypes. Here, we discuss recent findings rodent models establishing a role glia or regulation synaptic plasticity, brain metabolism, removal cellular debris immune challenges. These underscore relevance for benefits attributed have implications understanding neurobiological mechanisms underlying associated disorders.

Language: Английский

Citations

0
In vivo autofluorescence lifetime imaging of the Drosophila brain captures metabolic shifts associated with memory formation DOI Open Access

Philémon Roussel,

Mingyi Zhou, Chiara Stringari

et al.

Published: March 31, 2025

Neuronal energy regulation is increasingly recognized as a critical factor underlying brain functions and their pathological alterations, yet the metabolic dynamics that accompany cognitive processes remain poorly understood. As label-free minimally invasive technique, fluorescence lifetime imaging (FLIM) of coenzymes NADH NADPH (collectively referred to NAD(P)H) offers possibility resolve cellular profiles with high spatial precision. However, NAD(P)H FLIM’s capacity detect subtle changes in neuronal metabolism associated cognition has not been demonstrated. In this study, we applied FLIM map Drosophila neurons vivo across multiple scales, focusing on primary centers for associative memory: mushroom bodies (MBs). At broad scale, obtained an overview signatures main tissue identified marked difference between neuropil cortex areas. finer our findings revealed notable heterogeneity basal distinct MB neuron subtypes. Measurements performed after olfactory learning also uncovered subtype-specific shift memory formation, demonstrating utility detecting physiology-driven linked function. These results establish promising framework studying cerebral vivo.

Language: Английский

Citations

0
Starvation Induces Upregulation of Monocarboxylate Transport in Glial Cells at the Drosophila Blood–Brain Barrier DOI
Andrés González‐Gutiérrez, Jorge Gaete,

Andrés Esparza

et al.

Glia, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

ABSTRACT Living organisms can sense and adapt to constant changes in food availability. Maintaining a homeostatic supply of energy molecules is crucial for animal survival normal organ functioning, particularly the brain, due its high‐energy demands. However, mechanisms underlying brain adaptive responses availability have not been completely established. The nervous system separated from rest body by physical barrier called blood–brain (BBB). In addition structural role, BBB regulates transport metabolites nutrients into system. This regulation achieved through that control nutrients, including glucose monocarboxylates such as lactate, pyruvate, ketone bodies. Drosophila melanogaster , carbohydrate transporters increase their expression glial cells response starvation. or activity monocarboxylate (dMCTs) at yet reported. Here, we show neuronal ATP levels remain unaffected despite reduced energy‐related hemolymph larvae during Simultaneously, lactate beta‐hydroxybutyrate increases BBB. Using genetically encoded sensors, identified Yarqay proton‐coupled transporter whose upregulated subperineurial glia Our findings reveal novel component starvation: across BBB, mediated Yarqay, dMCT enriched

Language: Английский

Citations

0
Glial metabolism versatility regulates mushroom body–driven behavioral output inDrosophila DOI Open Access

Ruchira Basu,

Thomas Préat, Pierre-Yves Plaçais

et al.

Learning & Memory, Journal Year: 2024, Volume and Issue: 31(5), P. a053823 - a053823

Published: May 1, 2024

Providing metabolic support to neurons is now recognized as a major function of glial cells that conserved from invertebrates vertebrates. However, research in this field has focused for more than two decades on the relevance lactate and glycolysis neuronal energy metabolism, while overlooking many other facets metabolism their impact physiology, circuit activity, behavior. Here, we review recent work unveiled new features especially Drosophila , modulation behavioral traits involving mushroom bodies (MBs). These findings reveal spatially biochemically distinct modes glucose-derived fueling are implemented within MB memory type–specific manner. In addition, cortex glia endowed with several antioxidant functions, whereas astrocytes can serve pro-oxidant agents beneficial redox signaling underlying long-term memory. Finally, fatty acid oxidation seems play dual fail-safe role: first, mode production upon glucose shortage, and, second, factor clearance excessive oxidative load during sleep. Altogether, these integrated studies performed indicate deterministic role

Language: Английский

Citations

2