The effect of seasonal temperatures on the physiology of the overwintered honey bee DOI Creative Commons
Olga Frunze,

Yumi Yun,

Hyunjee Kim

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0315062 - e0315062

Published: Dec. 9, 2024

Honey bee physiology follows an annual cycle, with winter bees living ten times longer than summer bees. Their transition can be disrupted by climate change. Several factors, mainly temperature, may contribute to the global losses of We simulated warming maintaining constant temperatures 25°C (Group 25) and 35°C 35) in rooms around hives from June October, while a Group control experienced natural conditions. Colony performance was assessed August September. In February, workers were examined for physiological traits (acinus size lipid content fat body) molecular markers ( vg JHAMT ), along potential ilp1 , ilp2 TOR1 HSP70 ). Our findings suggest that temperature decreases worker broods 25 fall led their different states related aging compared 35 workers. Changes end diapause detected upregulation genes. These signs response could lead development strategies prevent improve identification insect models.

Language: Английский

Expression of Elongase‐ and Desaturase‐Encoding Genes Shapes the Cuticular Hydrocarbon Profiles of Honey Bees DOI Creative Commons
Daniel Sebastián Rodríguez‐León, Thomas Schmitt, M. Alice Pinto

et al.

Molecular Ecology, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Most terrestrial insects have a layer of cuticular hydrocarbons (CHCs) protecting them from desiccation and mediating chemical communication. The composition these is highly plastic changes during their lifetime with environmental conditions. How in CHC are achieved largely unknown. profiles Apis mellifera honey bees vary among castes, task groups subspecies adapted to different climates. This makes A. an excellent model for studying the molecular mechanism underlying biosynthesis. We correlated expression specific elongase- desaturase-encoding genes performing social tasks two divergent subspecies. Elongases enzymes that lengthen hydrocarbon chain, while desaturases introduce double bonds it. evaluated hypothesis encoding determines worker bees. Our results revealed specificity elongases shapes tasks. Expression gene LOC100576797 elongase-encoding LOC550828 seemed be strongly associated abundance compounds were characteristic profile nurse In contrast, characterised forager LOC551527 LOC409638. data shed light on genetic basis task-specific differences hymenopterans paved ground unravelling underpinning

Language: Английский

Citations

0
Honeybees fed D-galactose exhibit aging signs with changes in gut microbiota and metabolism DOI Creative Commons
Guanzhou Zhou, Jing Hu, Mengqi Xu

et al.

mSystems, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

ABSTRACT Honeybees ( Apis mellifera ), as social insects, exhibit complex behaviors and cognitive functions. The short lifespan stable gut microorganisms of honeybees provide certain availability a rapid high-flux animal model for aging research. This study explored the effect D-galactose, common inducer, on investigated associated effects mechanisms, with particular focus potential protective role sodium butyrate. Experimental cohorts were established follows: conventional (CV) group, D-galactose-treated (DG) butyrate-treated (SB) group. CV group was fed sucrose solution; DG D-galactose SB butyrate solution. A comprehensive assessment conducted day 15 post-treatment, including survival analysis, starvation test, motor, learning memory ability tests, malondialdehyde Smurf test. Potential mechanisms through microbiome metabolome investigated. Compared to from those in showed shortened lifespan, weaker energy storage ability, impaired learning, abilities, reduced weight, increased oxidation, disrupted barrier. These phenotypic changes microbial dysbiosis characterized by Lactobacillus enrichment diminished levels. Notably, supplementation extended honeybees’ improved their abilities damaged D-galactose. Our findings establish valuable system research highlight crucial metabolism senescence regulation. IMPORTANCE presents novel approach investigating processes establishing D-galactose-induced honeybees. demonstrate that effectively attenuates phenotypes, suggesting its therapeutic intervention age-related decline. provides unique studies highlights significant modulating progression. results contribute our understanding molecular underlying offer new insights into anti-aging strategies.

Language: Английский

Citations

0
Age dominates flight distance and duration, while body size shapes flight speed in Bombus terrestris L. (Hymenoptera: Apidae) DOI

Milena Gilgenreiner,

Christoph Kurze

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 291(2027)

Published: July 1, 2024

Flight plays a crucial role in the fitness of insect pollinators, such as bumblebees. Despite their relatively large body size compared with wings, bumblebees can fly under difficult ambient conditions, cooler temperatures. While is often positively linked to foraging range and flight ability, influence age remains less explored. Here, we studied performance (distance, duration speed) ageing bumblebee workers using tethered mills. Additionally, measured intertegular distance dry mass proxies for size. We found that were predominantly influenced by age, challenging assumptions does not play key task allocation. From 7 14 days, increased sixfold fivefold, respectively. Conversely, primarily impacted maximum average speed workers. Our findings indicate substantially influences workers, affecting potentially altering allocation strategies. This underscores importance considering individual physiological changes alongside size/mass experiments involving

Language: Английский

Citations

2
Deciphering the variation in cuticular hydrocarbon profiles of six European honey bee subspecies DOI Creative Commons
Daniel Sebastián Rodríguez‐León, Aleksandar Uzunov, Cecília Costa

et al.

BMC Ecology and Evolution, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 28, 2024

Abstract The Western honey bee ( Apis mellifera ) subspecies exhibit local adaptive traits that evolved in response to the different environments characterize their native distribution ranges. An important trait is cuticular hydrocarbon (CHC) profile, which helps prevent desiccation and mediate communication. We compared CHC profiles of six European A. m. , carnica ligustica macedonica iberiensis ruttneri investigated potential factors shaping composition. did not find evidence adaptation climatic conditions range. Subspecies-specific differences composition might be explained by phylogenetic constraints or genetic drift. foragers were more subspecies-specific than those nurse bees, while latter showed variation profiles, likely due lower stress exerted controlled environment inside hive. strongest profile appeared between bees among all subspecies, suggesting an social task a role Foragers also increase relative amount alkanes nurses, indicating conditions.

Language: Английский

Citations

2
Nosema ceranae infection reduces the fat body lipid reserves in the honeybee Apis mellifera DOI

Juliette Gilbert,

Laurianne Paris, Aurore Dubuffet

et al.

Journal of Invertebrate Pathology, Journal Year: 2024, Volume and Issue: 207, P. 108218 - 108218

Published: Oct. 10, 2024

Language: Английский

Citations

1
Expression of elongase- and desaturase-encoding genes shapes the cuticular hydrocarbon profiles of honey bees DOI Open Access
Daniel Sebastián Rodríguez‐León, Thomas Schmitt, M. Alice Pinto

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 23, 2024

Abstract Most terrestrial insects have a layer of cuticular hydrocarbons (CHCs) protecting them from desiccation and mediating chemical communication. CHC composition is regulated by the expression genes coding for enzymes in biosynthetic pathway hydrocarbons. The diversity these determine abundance richness compounds profile an insect. For example, elongases are that lengthen hydrocarbon chain, while desaturases introduce double bonds it. profiles honey bees ( Apis mellifera ) vary among castes, task groups, subspecies. This makes A. excellent model to study molecular mechanism underlying biosynthesis. Here, we examined specific elongase- desaturase-encoding correlated gene with two different groups highly divergent subspecies: m. carnica iberiensis . We show , specificity shapes groups. Our results shed light on genetic basis task-specific differences social hymenopterans lay ground further studies aiming unravel underpinning Moreover, underline importance investigating subspecies better understand mechanisms driving composition.

Language: Английский

Citations

0
Editorial Scheiner, Bloch "How do social insects know their tasks?" DOI
Ricarda Scheiner, Guy Bloch

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 66, P. 101257 - 101257

Published: Sept. 6, 2024

Language: Английский

Citations

0
Deciphering the variation in cuticular hydrocarbon profiles of five European honey bee subspecies DOI
Daniel Sebastián Rodríguez‐León, Aleksandar Uzunov, Cecília Costa

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 6, 2024

The Western honey bee ( Apis mellifera ) subspecies exhibit local adaptive traits that evolved in response to the different environments characterize their native distribution ranges. An important trait is cuticular hydrocarbon (CHC) profile, which helps preventing desiccation and mediating communication. We compared CHC profiles of six European A. m. , carnica ligustica macedonica iberiensis ruttneri investigated potential factors shaping composition. did not find evidence adaptation climatic conditions range. Subspecies-specific differences composition might be explained by phylogenetic constraints or genetic drift. foragers were more subspecies-specific than those nurse bees, while latter showed variation profiles, likely due lower stress exerted controlled environment inside hive. strongest profile appeared between bees among all subspecies, suggesting an social task a role Foragers also increase relative amount alkanes nurses, indicating conditions.

Language: Английский

Citations

0
Exploring formation of turanose in honey via stable isotope labelling and high-resolution mass spectrometry analysis DOI
Yuzhe Yuan, Sha Yan, Liming Wu

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 460, P. 140471 - 140471

Published: July 15, 2024

Language: Английский

Citations

0
The effect of seasonal temperatures on the physiology of the overwintered honey bee DOI Creative Commons
Olga Frunze,

Yumi Yun,

Hyunjee Kim

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0315062 - e0315062

Published: Dec. 9, 2024

Honey bee physiology follows an annual cycle, with winter bees living ten times longer than summer bees. Their transition can be disrupted by climate change. Several factors, mainly temperature, may contribute to the global losses of We simulated warming maintaining constant temperatures 25°C (Group 25) and 35°C 35) in rooms around hives from June October, while a Group control experienced natural conditions. Colony performance was assessed August September. In February, workers were examined for physiological traits (acinus size lipid content fat body) molecular markers ( vg JHAMT ), along potential ilp1 , ilp2 TOR1 HSP70 ). Our findings suggest that temperature decreases worker broods 25 fall led their different states related aging compared 35 workers. Changes end diapause detected upregulation genes. These signs response could lead development strategies prevent improve identification insect models.

Language: Английский

Citations

0