Wolbachia modify host cell metabolite profiles in response to short‐term temperature stress

Environmental Microbiology Reports, Journal Year: 2024, Volume and Issue: 16(5)

Published: Sept. 23, 2024

Abstract Wolbachia are common heritable endosymbionts that influence many aspects of ecology and evolution in various insects, yet ‐mediated intracellular metabolic responses to temperature stress have been largely overlooked. Here, we introduced the strain wLhui from invasive Liriomyza huidobrensis (Blanchard) into a Drosophila Schneider 2 cell line (S2) investigated metabolite profile wLhui‐infected (S2_wLhui) uninfected lines (S2_wu) under short‐term exposure either high (37°C), moderate (27°C), or low (7 17°C) temperatures. We find infection, stress, their interactions significantly affect cellular profiles. Most significantly, when comparing changes metabolites between S2_wLhui S2_wu, glycerophospholipids, amino acids, fatty acids associated with pathways, microbial metabolism diverse environments, other pathways were accumulated at Our findings suggest ‐induced physiological which may turn fitness adaptive ability its host as an species.

Language: Английский

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Sub-lethal pesticide exposure facilitates the potential northward range shifts of ticks by increasing cold tolerance and overwintering survival

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 19, 2025

Abstract Pesticides are a primary tool for the control of harmful insects or other organisms throughout world. Although most likely to encounter pesticides in environment, little is known about effects these chemicals on physiology off-target species. We measured impact sublethal pesticide exposure cold tolerance two common tick predicted that would make ticks more sensitive extreme temperatures. In contrast, we found had opposite effect, increasing survival at low Sublethal decreased LT50 (lethal temperature which 50% mortality observed) adult and nymphal D. variabilis from −16°C −19°C improved overwintering survival. Evaluation previously available RNA-seq data between rapid hardening indicated potential transcriptional shifts associated with cross-tolerance exposure. To investigate population-level impacts this physiological shift tolerance, developed novel approach incorporating into spatially explicit species distribution models (SDMs). Using approach, demonstrate pesticide-induced increases may permit faster northward range . also SDMs moderates estimated environmental change provide accurate predictions responses changing environments. our study limited ticks, studies have shown thermal traits diverse The extensive use drive complex interactions their environments, leading altered establishment new habitats.

Language: Английский

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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogaster

Journal of Insect Physiology, Journal Year: 2024, Volume and Issue: 159, P. 104701 - 104701

Published: Sept. 7, 2024

Language: Английский

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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogaster

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 28, 2024

Abstract In the cold, chill susceptible insects lose ability to regulate ionic and osmotic gradients. This leads hemolymph hyperkalemia that drives a debilitating loss of cell membrane polarization, triggering death pathways causing organismal injury. Biotic abiotic factors can modulate insect cold tolerance by impacting mitigate or prevent this cascade events. present study, we test combined isolated effects dietary manipulations thermal acclimation on in fruit flies. Specifically, acclimated adult Drosophila melanogaster 15 25°C fed them either K + -loaded diet control diet. We then tested these flies recover from survive exposure, as well their capacity protect transmembrane gradients, intracellular Na concentration. As predicted, cold-exposed experienced cold-acclimated had improved due an maintenance concentration at low temperature. Feeding high-K additively, but paradoxically reduced maintain extracellular concentrations. Cold-acclimation -feeding additively increased concentration, aiding gradient during exposure despite cold-induced hyperkalemia. There was no effect These findings suggest loading muscle sensitivity mechanisms through which -fed are able tolerate Highlights - Insect varies relation ionoregulatory Cold improves handling A high also tolerance, reduces -handling highlight novel mechanism for preventing disruption

Language: Английский

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Editorial Overview: Insect cold tolerance research reaches a Swift new Era

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: unknown, P. 101284 - 101284

Published: Oct. 1, 2024

Language: Английский

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