Recent advances of CRISPR-based genome editing for enhancing staple crops

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 23, 2024

An increasing population, climate change, and diminishing natural resources present severe threats to global food security, with traditional breeding genetic engineering methods often falling short in addressing these rapidly evolving challenges. CRISPR/Cas systems have emerged as revolutionary tools for precise modifications crops, offering significant advancements resilience, yield, nutritional value, particularly staple crops like rice maize. This review highlights the transformative potential of technology, emphasizing recent innovations such prime base editing, development novel CRISPR-associated proteins, which significantly improved specificity, efficiency, scope genome editing agriculture. These enable targeted that enhance tolerance abiotic stresses well biotic stresses. Additionally, plays a crucial role improving crop yield quality by enhancing photosynthetic nutrient uptake, resistance lodging, while also taste, texture, shelf life, content through biofortification. Despite challenges off-target effects, need more efficient delivery methods, ethical regulatory concerns, underscores importance security sustainability It calls continued research integration CRISPR other emerging technologies nanotechnology, synthetic biology, machine learning fully realize its developing resilient, productive, sustainable agricultural systems.

Language: Английский

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Synthesis, characterization, and computational evaluation of some synthesized xanthone derivatives: focus on kinase target network and biomedical properties

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 3, 2025

Xanthones are dubbed as putative lead-like molecules for cancer drug design and discovery. This study was aimed at the synthesis, characterization, in silico target fishing of novel xanthone derivatives. The products reactions xanthydrol with urea, thiourea, thiosemicarbazide reacted α-haloketones to prepare thiazolone compounds. Xanthydrol sequentially ethyl chloroacetate, hydrazine, carbon disulfide, dithiolane. propargyl bromide it submitted click reaction azide triazole ring. Finally, four xanthones derivatives including (E)-2-(2-(9H-xanthen-9-yl)hydrazono)-1,3-dithiolan-4-one (L3), 2-(2-(9H-xanthen-9-yl)hydrazinyl)thiazol-5(4H)-one (L5), 2-(9H-xanthen-9-ylamino)thiazol-5(4H)-one (L7), 4-((9H-xanthen-9-yloxy)methyl)-1-(4-nitrophenyl)-1H-1,2,3-triazole (L9) were synthesized characterized using thin layer chromatography, Fourier-transform infrared spectroscopy, nuclear magnetic resonance (1H 13C). ADMET, Pfizer filter, adverse reaction, toxicity, antitarget interaction profiles, fishing, kinase screening, molecular docking validation, protein gene network analysis computed Ligands obeyed filter drug-likeness, while all ligands categorized toxic chemicals. Major targets predicted be kinases Haspin, WEE2, PIM3. Mitogen-activated 1 hub All show hepatotoxic potentials, L7 presented cardiac toxicity. Acute leukemic T-cells one top tumor cell lines these ligands. possible antileukemic effects potentially very interesting warrant further studies.

Language: Английский

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Exploring the performance and mechanisms of Mychonastes rotundus in fixing CO2 based on multi-omics analysis

Journal of Cleaner Production, Journal Year: 2025, Volume and Issue: unknown, P. 144820 - 144820

Published: Jan. 1, 2025

Language: Английский

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Identification and functional characterization of UDP‐glycosyltransferase genes involved in cyetpyrafen resistance in Panonychus citri (McGregor)

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Cyetpyrafen is a newly developed acaricide. The citrus red mite, Panonychus citri (McGregor), an important pest that has resistance to cyetpyrafen. Uridine diphosphate-glycosyltransferases (UGTs) have been widely reported be associated with multiple acaricides. However, it rarely documented UGT genes participate in cyetpyrafen P. citri. In this study, significantly upregulated gene, PcUGT201E1, was identified using transcriptome analysis. Expression of PcUGT201E1 at all stages the cyetpyrafen-resistant strain and silencing increased susceptibility Molecular docking uridine diphosphate glucose (UDPG) indicated UDPG can interact PcUGT202E1 via hydrogen bonds. Heterologous expression vitro functional assays revealed enzyme activity could inhibited by recombinant deplete These results participates sequestration, provided molecular foundation for understanding © 2025 Society Chemical Industry.

Language: Английский

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Surface porosity-boosted fluorescence performance of N,N′-Bis(salicylidene)-1,3-propanediamine nanoparticles for thiabendazole detection: Sensing mechanism by DFT

Materials Chemistry and Physics, Journal Year: 2025, Volume and Issue: unknown, P. 130826 - 130826

Published: April 1, 2025

Language: Английский

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Functional redundancy in the toxic pathway of Bt protein Cry1Ab, but not Cry1Fa, against the Asian corn borer

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(16)

Published: April 17, 2025

Crops genetically engineered to produce insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) have been used extensively control some major crop pests, but their benefits decrease when pests evolve resistance. Better understanding of genetic basis resistance is needed effectively monitor, manage, and counter pest Bt crops. Resistance in at least 11 species Lepidoptera, including many important associated with naturally occurring mutations that disrupt one or more three larval midgut proteins: cadherin ATP-binding cassette ABCC2 ABCC3. Here, we determined how CRISPR/Cas9-mediated disrupting cadherin, ABCC2, ABCC3 singly pairs affect Cry1Ab Cry1Fa Asian corn borer ( Ostrinia furnacalis ), which most damaging Asia closely related European nubilalis a Europe North America. The results bioassays six knockout strains parent susceptible strain support model can kill larvae via path requiring another ABCC3, whereas uses only first path. model’s predictions are generally supported by linkage analyses responses Sf9 cells Xenopus oocytes modified pairs. functional redundancy identified here for could sustain its efficacy against O. may exemplify widespread natural strategy delaying

Language: Английский

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Omics approaches to unravel insecticide resistance mechanism in Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae)

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e17843 - e17843

Published: Sept. 5, 2024

(Gennadius) whitefly (BtWf) is an invasive pest that has already spread worldwide and caused major crop losses. Numerous strategies have been implemented to control their infestation, including the use of insecticides. However, prolonged insecticide exposures evolved BtWf resist these chemicals. Such resistance mechanism known be regulated at molecular level systems biology omics approaches could shed some light on understanding this regulation wholistically. In review, we discuss various techniques (genomics, transcriptomics, proteomics, metabolomics) unravel in BtWf. We summarize key genes, enzymes, metabolic are associated with review impact resistance. Evidently, enzymes involved detoxification system such as cytochrome P450 (CYP), glutathione S-transferases (GST), carboxylesterases (COE), UDP-glucuronosyltransferases (UGT), ATP binding cassette transporters (ABC) family played roles These genes/proteins can then serve foundation for other targeted techniques, gene silencing using RNA interference CRISPR. future, will useful knock down detoxifying genes crucial neutralizing mechanism, which lead solutions coping against infestation.

Language: Английский

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Overexpression of TuABCC4 is associated with abamectin resistance in Tetranychus urticae Koch

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

Pesticide resistance greatly limits control efficacy after the long-term application of pesticides. The two-spotted spider mite, Tetranychus urticae Koch, is a notorious agricultural pest worldwide that resistant to various pesticides, including abamectin. While some studies abamectin have investigated target related glutamate-gated chloride channels (GluCls), on metabolic mechanisms are still limited. In this study, we identified an ABCC subfamily gene, TuABCC4, was overexpressed in populations T. urticae, based analysis previously obtained transcriptomic and RNA-seq data. No consistent nonsynonymous mutations TuABCC4 gene were found between susceptible populations, although expression significantly increased all studied. Synergistic experiments with inhibitor verapamil confirmed key role resistance. addition, increase shown by RNA interference genetic association be closely conclusion, overexpression involved urticae. These results can help us better understand molecular basis

Language: Английский

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Comparative Genomics Uncovers the Evolutionary Dynamics of Detoxification and Insecticide Target Genes Across 11 Phlebotomine Sand Flies

Genome Biology and Evolution, Journal Year: 2024, Volume and Issue: 16(9)

Published: Sept. 1, 2024

Abstract Sand flies infect more than 1 million people annually with Leishmania parasites and other bacterial viral pathogens. Progress in understanding sand fly adaptations to xenobiotics has been hampered by the limited availability of genomic resources. To address this gap, we sequenced, assembled, annotated transcriptomes 11 phlebotomine species. Subsequently, leveraged these resources generate novel evolutionary insights pertaining their xenobiotics, including those contributing insecticide resistance. Specifically, over 2,700 detoxification genes conducted large-scale phylogenetic comparisons uncover dynamics five major gene families: cytochrome P450s (CYPs), glutathione-S-transferases (GSTs), UDP-glycosyltransferases (UGTs), carboxyl/cholinesterases (CCEs), ATP-binding cassette (ABC) transporters. Using comparative approach, show that have evolved diverse CYP GST repertoires, notable lineage-specific expansions groups evolutionarily related known xenobiotic metabolizers. Furthermore, conserved orthologs (i) CYP4G involved cuticular hydrocarbon biosynthesis, (ii) ABCB toxicity, (iii) two primary targets, acetylcholinesterase-1 (Ace1) voltage gated sodium channel (VGSC). The biological produced study provide a foundation for generating testing hypotheses regarding molecular mechanisms underlying xenobiotics.

Language: Английский

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Characterization of ATP‐binding cassette transporters associated with emamectin benzoate tolerance: from the model insect Drosophila melanogaster to the agricultural pest Spodoptera frugiperda

Pest Management Science, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 26, 2024

Abstract BACKGROUND Multiple families of detoxification genes, including the increasingly recognized family ATP‐binding cassette (ABC) transporters, work together to influence toxicity synthetic insecticides and thus their resistance. Effective management insecticide resistance requires identification all toxicity‐affecting members from each toxicity‐related genes. RESULTS Here, we used emamectin benzoate (EB), ABC transporters Spodoptera frugiperda as a working case test whether strategy ‘from model insect Drosophila melanogaster agricultural pests’ can identify or most transporter related EB tolerance in S. . After confirming involvement against fruit fly with inhibitor verapamil, four genes ( DmCG3327 , DmCG11147 DmCG4822 DmCG7627 ) were found be involved using RNA interference‐based family‐wide functional screening. A combination phylogenic analysis reciprocal TBLASTN search identified five homologs SfABCC4 SfABCG1 SfABCG23 one‐way best hits SfABCG4 SfABCG20 Real‐time quantitative polymerase chain reaction (qPCR) that inducible by EB, expressed developmental stages larval tissues, but significant differences among tissues. cytotoxicity assay ABC‐overexpressing Sf9 cell lines showed made cells tolerant EB. CONCLUSIONS This study not only identifies nine D. (four genes) (five genes), also demonstrates utility effectiveness ‘model given © 2024 Society Chemical Industry.

Language: Английский

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