Plantaginis Semen Ameliorates Hyperuricemia Induced by Potassium Oxonate DOI Open Access

Tian Liu,

Liting Wang,

Li Ji

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8548 - 8548

Published: Aug. 5, 2024

Plantaginis semen is the dried ripe seed of Plantago asiatica L. or depressa Willd., which has a long history in alleviating hyperuricemia (HUA) and chronic kidney diseases. While major chemical ingredients mechanism remained to be illustrated. Therefore, this work aimed elucidate chemicals working mechanisms PS for HUA. UPLC-QE-Orbitrap-MS was applied identify main components vitro vivo. RNA sequencing (RNA-seq) conducted explore gene expression profile, genes involved were further confirmed by real-time quantitative PCR (RT-qPCR). A total 39 identified from PS, 13 them detected rat serum after treating with PS. The tissue injury uric acid (UA), xanthine oxidase (XOD), cytokine levels reversed Meanwhile, renal urate anion transporter 1 (Urat1) glucose 9 (Glut9) treatment. RNA-seq analysis showed that PPAR signaling pathway; glycine, serine, threonine metabolism fatty pathway significantly modified Further, Slc7a8, Pck1, Mgll, Bhmt elevated, Fkbp5 downregulated, consistent results. Pparα, Pparγ, Lpl, Plin5, Atgl, Hsl elevated URAT1 PPARα proteins Western blotting. In conclusion, study elucidates profile prevention therapy HUA provides promising traditional Chinese medicine agency prophylaxis.

Language: Английский

Network pharmacology, a promising approach to reveal the pharmacology mechanism of Chinese medicine formula DOI
Li Zhao, Hong Zhang, Ning Li

et al.

Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 309, P. 116306 - 116306

Published: Feb. 27, 2023

Language: Английский

Citations

400
Network Pharmacology Approach for Medicinal Plants: Review and Assessment DOI Creative Commons
Fatima Noor, Muhammad Tahir ul Qamar, Usman Ali Ashfaq

et al.

Pharmaceuticals, Journal Year: 2022, Volume and Issue: 15(5), P. 572 - 572

Published: May 4, 2022

Natural products have played a critical role in medicine due to their ability bind and modulate cellular targets involved disease. Medicinal plants hold variety of bioactive scaffolds for the treatment multiple disorders. The less adverse effects, affordability, easy accessibility highlight potential traditional remedies. Identifying pharmacological from active ingredients medicinal has become hot topic biomedical research generate innovative therapies. By developing an unprecedented opportunity systematic investigation medicines, network pharmacology is evolving as paradigm becoming frontier field drug discovery development. advancement opened up new avenues understanding complex components found various plants. This study attributed comprehensive summary based on current research, highlighting ingredients, related techniques/tools/databases, development applications. Moreover, this would serve protocol discovering novel compounds explore full range biological traditionally used We attempted cover vast review form. hope it will significant pioneer researchers working with by employing approaches.

Language: Английский

Citations

240
Network pharmacology approaches for research of Traditional Chinese Medicines DOI
Xiang Li,

Ziqi LIU,

Jie Liao

et al.

Chinese Journal of Natural Medicines, Journal Year: 2023, Volume and Issue: 21(5), P. 323 - 332

Published: May 1, 2023

Language: Английский

Citations

150
Identification and virtual screening of novel umami peptides from chicken soup by molecular docking DOI
Jingcheng Zhang, Jincheng Zhang, Li Liang

et al.

Food Chemistry, Journal Year: 2022, Volume and Issue: 404, P. 134414 - 134414

Published: Sept. 27, 2022

Language: Английский

Citations

77
Network pharmacology: a crucial approach in traditional Chinese medicine research DOI Creative Commons
Yiyan Zhai,

Liu Liu,

Fanqin Zhang

et al.

Chinese Medicine, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 12, 2025

Abstract Network pharmacology plays a pivotal role in systems biology, bridging the gap between traditional Chinese medicine (TCM) theory and contemporary pharmacological research. enables researchers to construct multilayered networks that systematically elucidate TCM’s multi-component, multi-target mechanisms of action. This review summarizes key databases commonly used network pharmacology, including those focused on herbs, components, diseases, dedicated platforms for analysis. Additionally, we explore growing use TCM, citing literature from Web Science, PubMed, CNKI over past two decades with keywords like “network pharmacology”, “TCM “herb pharmacology”. The application TCM is widespread, covering areas such as identifying material basis efficacy, unraveling action, evaluating toxicity, safety, novel drug development. However, challenges remain, lack standardized data collection across insufficient consideration processed herbs Questions also persist regarding reliability study outcomes. aims offer valuable insights reference points guide future research precision pharmacology.

Language: Английский

Citations

5
Network pharmacology and experimental investigation of Rhizoma polygonati extract targeted kinase with herbzyme activity for potent drug delivery DOI Creative Commons
Yingqiu Xie,

Chengling Mu,

Bexultan Kazybay

et al.

Drug Delivery, Journal Year: 2021, Volume and Issue: 28(1), P. 2187 - 2197

Published: Jan. 1, 2021

(Huangjing, RP) has been used for a long history with many chemical components in inducing anti-cancer, anti-aging, anti-diabetes, anti-fatigue, and more prevention of diseases or acts as nutrition sources food. Here we investigated RP extract combination kinase inhibitors anti-cell growth blockade pathways targeting kinases. Experimental investigation network pharmacology analysis were applied to test the potent kinase-mediated signaling. Herbzyme activity was determined by substrate optical density measurement. Extract processed inhibits cell much greater manner than alone when mTOR EGFR. Moreover, processing methods from Mount Tai (RP-Mount Tai) play essential roles herbzyme phosphatase suggesting interface is also essential, addition component. The showed component target networks involving AKT mTOR, which consistent experimental validation. Finally, EGFR inhibitor could be associated nano-extract RP-Mount but not significantly affects

Language: Английский

Citations

72
Mechanisms by Which Traditional Chinese Medicines Influence the Intestinal Flora and Intestinal Barrier DOI Creative Commons

Qingya Che,

Tingting Luo,

Junhua Shi

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2022, Volume and Issue: 12

Published: April 28, 2022

The effect of a drug on the intestinal flora and barrier is an important evaluation index for safety efficacy. Chemical synthetic drugs are widely used due to their advantages fast efficacy low doses, but they prone cause resistance inhibit proton pumps, which may harm health. Traditional Chinese medicine (TCM) has been applied clinically thousands years, how TCMs regulate health achieve effects disease treatment become hot research topic that needs be resolved. This paper reviews recent microorganisms mucosal after entering intestine, discusses interaction mechanisms between flora, details repair provide reference development, utilization, modernization TCM.

Language: Английский

Citations

50
Challenges in phytotherapy research DOI Creative Commons
Alexander Panossian

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: May 31, 2023

OPINION article Front. Pharmacol., 31 May 2023Sec. Ethnopharmacology Volume 14 - 2023 | https://doi.org/10.3389/fphar.2023.1199516

Language: Английский

Citations

22
Simulation screening of umami peptides in the microbiota of soy sauce based on molecular docking and molecular dynamics DOI
Qi Wu,

Chunming Xu,

Yuan Tian

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 61, P. 104722 - 104722

Published: July 11, 2024

Language: Английский

Citations

8
Pharmacological network analysis of the functions and mechanism of kaempferol from Du Zhong in intervertebral disc degeneration (IDD) DOI Creative Commons
Xiaobin Wang, Yanlin Tan,

Fusheng Liu

et al.

Journal of Orthopaedic Translation, Journal Year: 2023, Volume and Issue: 39, P. 135 - 146

Published: March 1, 2023

Senescence and apoptosis of the nucleus pulposus cells (NPCs) are essential components intervertebral disc degeneration (IDD) process. anti-apoptosis treatments could be effective ways to delay or even stop degeneration. IDD has been treated with Eucommia ulmoides Oliver (Du Zhong, DZ) its active ingredients. However, roles mechanisms DZ in NPC senescence remain unclear. Traditional Chinese Medicine Systems Pharmacology (TCMSP) database was used select main ingredients threshold oral bioavailability (OB) ​≥ ​30% drug-likeness (DL) ​0.2. GSE34095 contained expression profile degenerative tissues non-degenerative were downloaded for different genes analysis. The disease targets retrieved from GeneCards. online tool Metascape functional enrichment annotation specific effects ingredient on IL-1β cell proliferation, senescence, reactive oxygen species (ROS) accumulation determined by CCK-8, SA-β-gal staining, flowcytometry western blot assays. A total 8 compounds found meet OB DL ​0.2 4151 drug targets. After intersection 879 obtained GeneCards 230 DEGs IDD-related GSE dataset, a 13 hub overlapped. According analysis Metascape, these showed dramatically enriched AGE-RAGE signaling, proteoglycans cancer, wound healing, transmembrane receptor protein tyrosine kinase MAPK cascades, ERK1/2 PI3K/Akt signaling pathway, skeletal system, etc. Disease association DisGeNET indicated that significantly associated IDD, disease, dysplasia, other diseases. Active ingredients-targets-signaling pathway networks constructed Cytoscape, kaempferol identified as compound DZ. In IL-1β-induced vitro model, treatment improved viability suppression senescence. addition, attenuated ROS apoptosis. Furthermore, partially eliminated decreases aggrecan, collagen II, SOX9, FN1 levels increases MMP3, MMP13, ADAMTS-4, ADAMTS-5. Moreover, relieved promotive stimulation upon p38, JNK, phosphorylation. inhibitor PD0325901 further enhanced effect inhibition phosphorylation, downregulation MMP3 ADAMTS-4 expression, upregulation aggrecan II expressions. Kaempferol regarded major DZ, protecting NPCs damages through promoting viability, inhibiting apoptosis, increasing ECM production, decreasing degradation. may involved. This study provides experimental data support pharmacological treating lays solid foundation future clinical application treatment.

Language: Английский

Citations

22