Integrating Serum Pharmacochemistry With Network Pharmacology to Elucidate the Mechanism of Wushen Decoction in the Prevention and Treatment of Lower Extremity Erysipelas DOI

Ying Wang,

Xiao Dai,

Xianhong Zhu

et al.

Biomedical Chromatography, Journal Year: 2025, Volume and Issue: 39(5)

Published: April 10, 2025

ABSTRACT Lower extremity erysipelas (LEE), a frequently seen skin and soft tissue infection caused predominantly by streptococci, usually presents with fever, erythema pain. Wushen Decoction (WSD), Compound traditional Chinese medicine, has been used historically to treat LEE, though its exact mechanism of action remains unclear. In this study, we explored the therapeutic mechanisms WSD in treating LEE employing combination serum pharmacochemistry, network pharmacology, molecular docking techniques. Initially, using UPLC‐Q‐Exactive Orbitrap‐MS/MS, 39 candidate active compounds rats treated were identified. Subsequently, pharmacology analysis identified 35 overlapping targets between components, 23 related signaling pathways. Further studies have confirmed that key components (rutin, hyperoside luteoloside) possess potential for effective effects core (PTGS 2 TNF). Furthermore, vitro experiments demonstrated significantly downregulated expression PTGS TNF, thereby validating findings providing insights into mechanisms. Results suggested may exert on modulating TNF NF‐kappa B pathway, offering promising approach prevention treatment LEE.

Language: Английский

Involvement of SIRT1-mediated aging in liver diseases DOI Creative Commons
Yueming Zhang, Chang Gong, Lina Tao

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 20, 2025

Liver disease is a significant global health issue, responsible for millions of deaths annually. Aging, characterized by the gradual decline in cellular and physiological functions, impairs tissue regeneration, increases susceptibility to liver diseases, leads health. Silent information regulator 1 (SIRT1), NAD⁺-dependent deacetylase, has emerged as pivotal factor modulating age-related changes liver. SIRT1 preserves function regulating essential aging-related pathways, including telomere maintenance, epigenetic modifications, senescence, intercellular communication, inflammation, mitochondrial function. Notably, levels naturally with age, contributing progression increased vulnerability injury. This review summarizes regulatory role aging its impact on diseases such fibrosis, alcoholic associated (ALD), metabolic dysfunction-associated steatotic (MASLD), steatohepatitis (MASH), hepatocellular carcinoma (HCC). We also discuss emerging therapeutic approaches, activators, gene therapy, nutritional interventions, which are evaluated their potential restore mitigate progression. Finally, we highlight future research directions optimize SIRT1-targeted therapies clinical applications conditions.

Language: Английский

Citations

1
Design and interaction mechanism of novel SIRT1 inhibitors for the treatment of hepatocellular carcinoma DOI
Jing Luo, Xiaomeng Wang, Qiao Fu

et al.

New Journal of Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Silent information regulator sirtuin 1 (SIRT1) is a niacinamide adenine dinucleotide (NAD)-dependent histone deacetylase and promising target for the treatment of hepatocellular carcinoma (HCC).

Language: Английский

Citations

0
Recent advancement in prevention against hepatotoxicity, molecular mechanisms, and bioavailability of gallic acid, a natural phenolic compound: challenges and perspectives DOI Creative Commons
Peng Chen, Fan Zou, Wei Liu

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 21, 2025

Drug-induced liver injury (DILI) results from the toxicity caused by drugs or their metabolites. Gallic acid (GA) is a naturally occurring secondary metabolite found in many fruits, plants, and nuts. Recently, GA has drawn increasing attention due to its potent pharmacological properties, particularly anti-inflammatory antioxidant capabilities. To best of our knowledge, this first review focus on properties related molecular activation mechanisms regarding protection against hepatotoxicity. We also provide thorough explanation physicochemical fruit sources, toxicity, pharmacokinetics after reviewing substantial number studies. Pharmacokinetic studies have shown that quickly absorbed eliminated when taken orally, which restricts use development. However, bioavailability can be increased optimizing structure changing form administration. Notably, according toxicology conducted range animals clinical trials, rarely exhibits side effects. The antioxidation mainly involved Nrf2, while MAPKs NF-κB signaling pathways. Owing marked prospective candidate for management diverse xenobiotic-induced discuss applications cutting-edge technologies (nano-delivery systems, network pharmacology, organoids) DILI. In addition guiding future research development as medicine, study offers theoretical foundation application.

Language: Английский

Citations

0
Bioinformatics analysis and experimental validation of potential targets and pathways in chronic kidney disease associated with renal fibrosis DOI Creative Commons

Cui Huimin,

Zhao Yuxin,

Peng Wang

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 2, 2025

Chronic kidney disease (CKD) has emerged as a major health problem worldwide. Previous studies have shown that specific miRNA expression profiles of patients with CKD are significantly changed. In this study, we aim to elucidate the role miRNAs potential biomarkers in progression by integrating bioinformatics analysis experimental validation, thereby providing medical evidence for prevention and treatment CKD. Bioinformatics was used identify targets pathways CKD-associated renal fibrosis through randomly obtaining microarray data related Gene Expression Omnibus (GEO) database according inclusion exclusion criteria, conducting pathway enrichment constructing protein-protein interaction (PPI) networks miRNA-mRNA network Cytoscape 3.8.0. vitro experiments were employed verify mechanism miR-223-3p human tubular epithelial cells (HK2) Quantitative real-time PCR assays, Western blot, Immunofluorescence Double luciferase reporter gene experiment. Multi-group one-way variance (ANOVA) Dunnett-t test uesd analyze results SPSS24.0. 10 up-regulated 11 down-regulated screened out. Phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) first analysis. MiR-223-3p (logFC=-2.047, p = 0.002) one four hub miRNAs. Furthermore, observed reduction α-smooth muscle actin (α-SMA) (p 0.001) Collagen type I alpha 1 (Col1-a1) 0.023) levels upon overexpression, which aligned our predictions. This downregulation attributed inhibition nuclear factor kappa-B (NF-κB) translocation subsequent decrease secretion inflammatory cytokines, such interleukin-6 (IL-6) 0.005). Conversely, when CHUK further overexpressed, inhibitory effect on epithelial-mesenchymal transition (EMT) attenuated, confirming between CHUK. Our findings provide compelling acts suppressor EMT specifically targeting modulating PI3K/Akt pathway, holds great promise novel therapeutic target treatment. Additionally, study offers avenue development future interventions aimed at halting or reversing

Language: Английский

Citations

0
Integrating Serum Pharmacochemistry With Network Pharmacology to Elucidate the Mechanism of Wushen Decoction in the Prevention and Treatment of Lower Extremity Erysipelas DOI

Ying Wang,

Xiao Dai,

Xianhong Zhu

et al.

Biomedical Chromatography, Journal Year: 2025, Volume and Issue: 39(5)

Published: April 10, 2025

ABSTRACT Lower extremity erysipelas (LEE), a frequently seen skin and soft tissue infection caused predominantly by streptococci, usually presents with fever, erythema pain. Wushen Decoction (WSD), Compound traditional Chinese medicine, has been used historically to treat LEE, though its exact mechanism of action remains unclear. In this study, we explored the therapeutic mechanisms WSD in treating LEE employing combination serum pharmacochemistry, network pharmacology, molecular docking techniques. Initially, using UPLC‐Q‐Exactive Orbitrap‐MS/MS, 39 candidate active compounds rats treated were identified. Subsequently, pharmacology analysis identified 35 overlapping targets between components, 23 related signaling pathways. Further studies have confirmed that key components (rutin, hyperoside luteoloside) possess potential for effective effects core (PTGS 2 TNF). Furthermore, vitro experiments demonstrated significantly downregulated expression PTGS TNF, thereby validating findings providing insights into mechanisms. Results suggested may exert on modulating TNF NF‐kappa B pathway, offering promising approach prevention treatment LEE.

Language: Английский

Citations

0