Nature Communications,
Journal Year:
2017,
Volume and Issue:
8(1)
Published: Nov. 15, 2017
Upon
mating,
fruit
fly
females
become
refractory
to
further
mating
for
several
days.
An
ejaculate
protein
called
sex
peptide
(SP)
acts
on
uterine
neurons
trigger
this
behavioural
change,
but
it
is
still
unclear
how
the
SP
signal
modifies
decision.
Here
we
describe
two
groups
of
female-specific
local
interneurons
that
are
important
process-the
ventral
abdominal
lateral
(vAL)
and
medial
(vAM)
interneurons.
Both
vAL
vAM
express
myoinhibitory
(Mip)-GAL4.
positive
Mip
neuropeptides
sex-determining
transcriptional
factor
doublesex.
Silencing
in
induces
active
rejection
male
courtship
attempts,
whereas
activation
makes
even
mated
receptive
re-mating.
located
ganglion
(AG)
where
they
relay
other
AG
project
brain.
appear
promote
receptivity
both
virgins
females,
although
dispensable
normal
virgin
females.
The
neural
circuits
responsible
for
animal
behavior
remain
largely
unknown.
We
summarize
new
methods
and
present
the
circuitry
of
a
large
fraction
brain
fruit
fly
Drosophila
melanogaster
.
Improved
include
procedures
to
prepare,
image,
align,
segment,
find
synapses
in,
proofread
such
data
sets.
define
cell
types,
refine
computational
compartments,
provide
an
exhaustive
atlas
examples
many
them
novel.
detailed
consisting
neurons
their
chemical
most
central
brain.
make
public
simplify
access,
reducing
effort
needed
answer
circuit
questions,
linking
defined
by
our
analysis
with
genetic
reagents.
Biologically,
we
examine
distributions
connection
strengths,
motifs
on
different
scales,
electrical
consequences
compartmentalization,
evidence
that
maximizing
packing
density
is
important
criterion
in
evolution
fly’s
Nature Communications,
Journal Year:
2016,
Volume and Issue:
7(1)
Published: June 13, 2016
Abstract
How
organisms
adapt
to
new
environments
is
of
fundamental
biological
interest,
but
poorly
understood
at
the
genetic
level.
Chemosensory
systems
provide
attractive
models
address
this
problem,
because
they
lie
between
external
environmental
signals
and
internal
physiological
responses.
To
investigate
how
selection
has
shaped
well-characterized
chemosensory
system
Drosophila
melanogaster
,
we
have
analysed
genome-wide
data
from
five
diverse
populations.
By
couching
population
genomic
analyses
protein
families
within
parallel
other
large
families,
demonstrate
that
proteins
are
not
outliers
for
adaptive
divergence
species.
However,
often
display
strongest
recent
D.
.
We
show
adaptation
operated
almost
exclusively
on
standing
variation,
patterns
mutations
predict
effects
function.
Finally,
evidence
experienced
relaxed
constraint,
argue
been
important
their
rapid
over
short
timescales.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2020,
Volume and Issue:
unknown
Published: Jan. 21, 2020
Abstract
The
neural
circuits
responsible
for
behavior
remain
largely
unknown.
Previous
efforts
have
reconstructed
the
complete
of
small
animals,
with
hundreds
neurons,
and
selected
larger
animals.
Here
we
(the
FlyEM
project
at
Janelia
collaborators
Google)
summarize
new
methods
present
circuitry
a
large
fraction
brain
much
more
complex
animal,
fruit
fly
Drosophila
melanogaster
.
Improved
include
procedures
to
prepare,
image,
align,
segment,
find
synapses,
proofread
such
data
sets;
that
define
cell
types
based
on
connectivity
in
addition
morphology;
simplify
access
evolving
set.
From
resulting
derive
better
definition
computational
compartments
their
connections;
an
exhaustive
atlas
examples
types,
many
them
novel;
detailed
most
central
brain;
exploration
statistics
structure
different
compartments,
as
whole.
We
make
public,
web
site
resources
specifically
designed
it
easy
explore,
all
levels
expertise
from
expert
merely
curious.
public
availability
these
data,
simplified
means
it,
dramatically
reduces
effort
needed
answer
typical
circuit
questions,
identity
upstream
downstream
partners,
regions,
link
neurons
defined
by
our
analysis
genetic
reagents
can
be
used
study
functions.
Note:
In
next
few
weeks,
will
release
series
papers
involved
discussions.
One
paper
detail
hemibrain
reconstruction
extensive
interpretation
made
possible
this
dense
connectome.
Another
explore
complex,
region
navigation,
motor
control,
sleep.
A
final
insights
mushroom
body,
center
multimodal
associative
learning
brain.
Cell,
Journal Year:
2023,
Volume and Issue:
186(12), P. 2556 - 2573.e22
Published: May 25, 2023
In
Drosophila,
a
dedicated
olfactory
channel
senses
male
pheromone,
cis-vaccenyl
acetate
(cVA),
promoting
female
courtship
while
repelling
males.
Here,
we
show
that
separate
cVA-processing
streams
extract
qualitative
and
positional
information.
cVA
sensory
neurons
respond
to
concentration
differences
in
5-mm
range
around
male.
Second-order
projection
encode
the
angular
position
of
by
detecting
inter-antennal
concentration,
which
are
amplified
through
contralateral
inhibition.
At
third
circuit
layer,
identify
47
cell
types
with
diverse
input-output
connectivity.
One
population
responds
tonically
flies,
second
is
tuned
looming,
integrates
taste
coincidentally
promote
mating.
The
separation
features
resembles
mammalian
what
where
visual
streams;
together
multisensory
integration,
this
enables
behavioral
responses
appropriate
specific
ethological
contexts.
Cell,
Journal Year:
2019,
Volume and Issue:
178(4), P. 901 - 918.e16
Published: Aug. 1, 2019
Physiology
and
metabolism
are
often
sexually
dimorphic,
but
the
underlying
mechanisms
remain
incompletely
understood.
Here,
we
use
intestine
of
Drosophila
melanogaster
to
investigate
how
gut-derived
signals
contribute
sex
differences
in
whole-body
physiology.
We
find
that
carbohydrate
handling
is
male-biased
a
specific
portion
intestine.
In
contrast
known
sexual
dimorphisms
invertebrates,
intestinal
extrinsically
controlled
by
adjacent
male
gonad,
which
activates
JAK-STAT
signaling
enterocytes
within
this
portion.
Sex
reversal
experiments
establish
roles
for
metabolic
state
controlling
food
intake
sperm
production
through
citrate.
Our
work
uncovers
gonad-gut
axis
coupling
diet
production,
revealing
communication
across
organs
physiologically
important.
The
instructive
role
citrate
inter-organ
might
be
significant
more
biological
contexts
than
previously
recognized.
