Microbiological Research, Journal Year: 2022, Volume and Issue: 261, P. 127078 - 127078
Published: May 26, 2022
Language: Английский
Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14
Published: June 23, 2023
With an ever-growing human population, the need for sustainable production of nutritional food sources has never been greater. Aquaculture is a key industry engaged in active development to increase line with this while remaining terms environmental impact and promoting good welfare health farmed species. Microbiomes fundamentally underpin animal health, being part their digestive, metabolic defense systems, latter case protecting against opportunistic pathogens environment. The potential manipulate microbiome advantage enhancing intriguing prospect that gained considerable traction recent years. In review we first set out what known about role aquaculture systems across phylogenetic spectrum cultured animals, from invertebrates finfish. view reducing footprint tightening biological physical control, investment “closed” on rise, but little how microbial these closed affect organisms. Through comparisons microbiomes dynamics phylogenetically distinct animals different focus communities functionality order identify features within be harnessed optimizing healthy intensified support future aquaculture.
Language: Английский
Citations
28
Journal of Hazardous Materials Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100612 - 100612
Published: Jan. 1, 2025
Language: Английский
Citations
1
Microbial Ecology, Journal Year: 2023, Volume and Issue: 86(3), P. 1755 - 1772
Published: Feb. 22, 2023
Pacific oysters (Crassostrea gigas) and Mediterranean mussels (Mytilus galloprovincialis) are commercially important marine bivalves that frequently coexist have overlapping feeding ecologies. Like other invertebrates, their gut microbiota is thought to play an role in supporting health nutrition. Yet, little known regarding the of host environment driving these communities. Here, bacterial assemblages were surveyed from seawater aspirates farmed C. gigas co-occurring wild M. galloprovincialis summer winter using Illumina 16S rRNA gene sequencing. Unlike seawater, which was dominated by Pseudomonadata, bivalve samples largely consisted Mycoplasmatota (Mollicutes) accounted for >50% total OTU abundance. Despite large numbers common (core) taxa, bivalve-specific species (OTUs) also evident predominantly associated with Mycoplasmataceae (notably Mycoplasma). An increase diversity (though varied taxonomic evenness) observed both changes abundance core including several representing host-associated environmental (free-living or particle-diet associated) organisms. Our findings highlight contribution defining composition cohabiting, intergeneric populations.
Language: Английский
Citations
17
Reviews in Aquaculture, Journal Year: 2024, Volume and Issue: 16(3), P. 1229 - 1243
Published: Feb. 15, 2024
Abstract The holobiont theory expands the notion of individual multicellular organisms as a community composed host and all its associated microorganisms. This concept has been extensively studied in field aquaculture, where increasing evidence highlighted importance microorganisms species fitness. Here, we focus our review on mollusc crustacean which microbiota dysbiosis recently described context various diseases, resulting significant economic losses. Influencing structure through use probiotics is potential strategy that could improve fitness or robustness cultivated species. We discuss here possibility developing microbiome targeted prophylactic approaches by promoting (1) methods to identify microbial fosters good health status (2) early life education favour long‐term resistance stress disease. aims inform aquaculture industry about strategies rearing practices mitigate diseases
Language: Английский
Citations
7
Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1901)
Published: March 18, 2024
The Pacific oyster Crassostrea gigas lives in microbe-rich marine coastal systems subjected to rapid environmental changes. It harbours a diversified and fluctuating microbiota that cohabits with immune cells expressing gene repertoire. In the early stages of development, just after fertilization, plays key role educating system. Exposure rich microbial environment at larval stage leads an increase competence throughout life oyster, conferring better protection against pathogenic infections later juvenile/adult stages. This beneficial effect, which is intergenerational, associated epigenetic remodelling. At juvenile stages, educated system participates control homeostasis. particular, fine-tuned by antimicrobial peptides acting through specific synergistic effects. However, this balance fragile, as illustrated Oyster Mortality Syndrome, disease causing mass mortalities oysters worldwide. disease, weakening defences OsHV-1 µVar virus induces dysbiosis leading fatal sepsis. review illustrates continuous interaction between highly its dynamic life, importance cross-talk for health. article part theme issue ‘Sculpting microbiome: how host factors determine respond colonization’.
Language: Английский
Citations
7
Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(4)
Published: March 11, 2024
ABSTRACT Pacific oysters ( Magallana gigas, a.k.a. Crassostrea gigas ), the most widely farmed oysters, are under threat from climate change and emerging pathogens. In part, their resilience may be affected by microbiome, which, in turn, influenced ocean warming acidification. To understand these impacts, we exposed early-development oyster spat to different temperatures (18°C 24°C) p CO 2 levels (800, 1,600, 2,800 µatm) a fully crossed design for 3 weeks. Under all conditions, microbiome changed over time, with large decrease relative abundance of potentially pathogenic ciliates Uronema marinum ) treatments time. The composition differed significantly temperature, but not acidification, indicating that microbiomes can altered is resilient acidification our experiments. Microbial taxa implying adaptive strategies ecological specializations among microorganisms. Additionally, small proportion (~0.2% total taxa) relatively abundant microbial were core constituents (>50% occurrence samples) across temperatures, levels, or Some taxa, including A4b bacteria members family Saprospiraceae phyla Chloroflexi (syn. Chloroflexota Bacteroidetes Bacteroidota respectively, as well protists genera Labyrinthula Aplanochytrium class Labyrinthulomycetes , Pseudoperkinsus tapetis Ichthyosporea suggesting they play an important, albeit unknown, role maintaining structural functional stability response These findings highlight flexibility environmental changes. IMPORTANCE economically important species oyster, production depends on healthy spat. health productivity associated microbiota; yet, studies have scrutinized effects temperature prokaryotic eukaryotic Here, show both and, first surprisingly changes sensitive warming. potential implications survival amid underscore need cascading productivity.
Language: Английский
Citations
5
Journal of Applied Microbiology, Journal Year: 2024, Volume and Issue: 135(4)
Published: April 1, 2024
Abstract Aims This study investigated phenotypic and genotypic antimicrobial resistance profiles of Vibrio strains identified from Mytilus galloprovincialis farmed for human consumption in the Adriatic Sea Central Italy. Methods results A total 475 mussels (M. galloprovincialis) were involved present study, culture-dependent microbiological methods permitted to identify a 50 that tested antibiotic susceptibility followed by genetic determinant detections. Antibiograms showed against ampicillin (36.0%), amoxicillin-clavulanic acid (30.0%), gentamycin (14.0%), imipenem (18.0%). Biomolecular assays amplified 264 genes harbored both susceptible resistant species. Among genes, aacC2 (62.0%) aadA (58.0%) aminoglycosides, blaTEM (54.0%) beta-lactams, qnrS (24.0%) quinolones, tetD (66.0%) tetracyclines, vanB (60.0%) glycopeptides mainly PCR assays. Conclusions genus is phenomenon diffusion aquatic environments, as demonstrated harboring many determinants representing kind “dark world”.
Language: Английский
Citations
4
Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15
Published: Jan. 8, 2025
Metagenomic sequencing is increasingly being employed to understand the assemblage and dynamics of oyster microbiome. Specimen collection processing steps can impact resultant microbiome composition introduce bias. To investigate this systematically, a total 54 farmed oysters were collected from Chesapeake Bay between May September 2019. Six different specimen types methods evaluated for microbial community using shotgun metagenomics, namely fresh homogenate (FOH), after simulated temperature abuse (AOH), Luria broth-enriched (EOH), dissected stomach (DSH), hemolymph (HLM), stomach-gut content (SGC). In general, DSH, EOH, FOH yielded highest DNA concentration, while EOH had reads, followed by HLM, FOH. HLM produced bacterial species alpha diversity, AOH, SGC. Although diversities did not differ significantly, beta-diversity measurements showed significant dissimilarity among (p < 0.05) indicating that do play an important role in representing community. Bacterial log mean abundance included Cyanobium sp. PCC 7001 FOH, Vibrio vulnificus lastly Synechococcus CB0205 HML, SGC samples. displayed higher hits, distinct composition, values bacterial, phages, antimicrobial resistance gene reads. Therefore, if studying overall community, prioritizing optimum align with goal study recommended.
Language: Английский
Citations
0
Aquatic Toxicology, Journal Year: 2025, Volume and Issue: 279, P. 107255 - 107255
Published: Jan. 24, 2025
Ecotoxicological risk and the mode of action human drugs on non-target marine animals remain unclear, keeping a gap knowledge risks related to ecosystem disruption chemical contamination food chains. Understanding these impacts is critical developing proper waste management practices regulatory frameworks prevent long-term environmental health problems. This study investigates Gemfibrozil, Metformin, Ramipril, Venlafaxine, individually combined Mytilus galloprovincialis over 30 days assesses persistent effects post-recovery using RNA-seq 16S rRNA microbiota profiling. All pharmaceuticals caused few changes in while gene expression analyses highlighted drug-specific alterations. Gemfibrozil exposure led alterations lipid fatty acid metabolism, suggesting similar that observed target species. Metformin significantly impacted mussels' energy with disruptions specific genes pathways potentially glucose uptake insulin signaling. was also treatment leading most significant predicted functional profiles microbiota, it may influence microbiota's potential interact host metabolism. Ramipril resulted up-regulation stress response cell cycle regulation Venlafaxine induced serotonin synapse pathways, indicating similarities mechanisms Mixture four severely mussel physiology, including impairment oxidative phosphorylation compensatory activation several involved Despite recovery after depuration, metabolism suggests from pharmaceutical exposure. Notably, mTOR1 signaling all treatments underscores its key role coordinating bivalve responses. The Transcriptomic Hazard Index (THI) calculated for each indicates major/severe hazards decreased slight/moderate depuration.
Language: Английский
Citations
0
ISME Communications, Journal Year: 2025, Volume and Issue: 5(1)
Published: Jan. 1, 2025
Abstract Giant clams, with their significant ecological importance, depend on associated bacteria for health and development, yet the transmission modes succession of community dynamics these remain poorly understood. This study employed 16S rRNA gene sequencing microscopy to investigate symbiotic in giant clam Tridacna crocea during early developmental stages (fertilized eggs, blastocyst, D-larvae, pediveliger larvae). Fluorescence situ hybridization electron did not detect internal fertilized eggs adult gonad gametes, but scanning revealed microbial structures egg surface microvilli, suggesting role as carriers. confirmed presence indicating bacterial acquisition via external vertical (adherence microvilli) or horizontal transmission. Given lack internalized reproductive organs, we prefer classify Microbial analysis showed that T. acquired a portion its microbiome from seawater throughout development. Before reaching stage, composition closely resembled surrounding seawater, primarily featuring family Rhodobacteraceae. As matured, host’s selective pressure increased (e.g. deterministic assembly), which simplified reduced diversity. During genus Endozoicomonas became dominant, forming large proportion within gonads. highlights significance host–microbe interactions maintaining biodiversity driving ecosystem stability through dynamic assembly processes.
Language: Английский
Citations
0