bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 8, 2024
ABSTRACT
Microbes
can
be
engineered
to
detect
target
chemicals,
but
when
they
operate
in
real-world
environments,
it
remains
unclear
how
competition
with
natural
microbes
affect
their
performance
over
long
time
periods.
We
sensors
and
memory-storing
genetic
circuits
inside
Bacillus
subtilis
sense
respond
the
TNT
explosive,
using
predictive
models
for
rational
design.
characterized
ability
a
soil
system,
measuring
single-cell
population-level
behavior
28-day
period.
The
autonomous
microbial
sensor
activated
its
response
by
14-fold
exposed
low
concentrations
maintained
stable
activation
21
days,
exhibiting
exponential
decay
dynamics
at
half-life
of
about
5
days.
Our
results
show
that
bacteria
carry
out
long-term
detection
an
important
chemical
competitive
growth
serving
as
additional
biocontainment.
European Journal of Pharmaceutical Sciences,
Journal Year:
2023,
Volume and Issue:
188, P. 106515 - 106515
Published: July 2, 2023
Microbial
resistance
has
increased
in
recent
decades
as
a
result
of
the
extensive
and
indiscriminate
use
antibiotics.
The
World
Health
Organization
listed
antimicrobial
one
ten
major
global
public
health
threats
2021.
In
particular,
six
bacterial
pathogens,
including
third-generation
cephalosporin-resistant
Escherichia
coli,
methicillin-resistant
Staphylococcus
aureus,
carbapenem-resistant
Acinetobacter
baumannii,
Klebsiella
pneumoniae,
Streptococcus
Pseudomonas
aeruginosa,
were
found
to
have
highest
resistance-related
death
rates
2019.
To
respond
this
urgent
call,
creation
new
pharmaceutical
technologies
based
on
nanoscience
drug
delivery
systems
appears
be
promising
strategy
against
microbial
light
advancements,
particularly
knowledge
medicinal
biology.
Nanomaterials
are
often
defined
substances
having
sizes
between
1
100
nm.
If
material
is
used
small
scale;
its
properties
significantly
change.
They
come
variety
forms
help
provide
distinguishing
characteristics
for
wide
range
functions.
field
sciences
demonstrated
strong
interest
numerous
nanotechnology
applications.
Therefore,
review,
prospective
nanotechnology-based
therapeutics
management
infections
with
multiple
medication
critically
examined.
Recent
developments
these
innovative
treatment
techniques
described,
an
emphasis
preclinical,
clinical,
combinatorial
approaches.
British Journal of Biomedical Science,
Journal Year:
2024,
Volume and Issue:
81
Published: Aug. 7, 2024
The
global
issue
of
antimicrobial
resistance
poses
significant
challenges
to
public
health.
World
Health
Organization
(WHO)
has
highlighted
it
as
a
major
health
threat,
causing
an
estimated
700,000
deaths
worldwide.
Understanding
the
multifaceted
nature
antibiotic
is
crucial
for
developing
effective
strategies.
Several
physiological
and
biochemical
mechanisms
are
involved
in
development
resistance.
Bacterial
cells
may
escape
bactericidal
actions
drugs
by
entering
physiologically
dormant
state
known
bacterial
persistence.
Recent
findings
this
field
suggest
that
persistence
can
be
one
main
sources
chronic
infections.
tolerance
developed
persister
could
tolerate
high
levels
antibiotics
give
rise
offspring.
These
offspring
attributed
mechanisms,
especially
This
review
attempts
shed
light
on
persister-induced
current
therapeutic
ABSTRACT
Biofilms
provide
individual
bacteria
with
many
advantages,
yet
dense
cellular
proliferation
can
also
create
intrinsic
metabolic
challenges
including
excessive
acidification.
Because
such
pH
stress
be
masked
in
buffered
laboratory
media—such
as
MSgg
commonly
used
to
study
Bacillus
subtilis
biofilms—it
is
not
always
clear
how
biofilms
cope
minimally
natural
environments.
Here,
we
report
B.
overcome
this
challenge
through
an
active
regulation
mechanism.
Specifically,
find
that
these
modulate
their
extracellular
the
preferred
neutrophile
range,
even
when
starting
from
acidic
and
alkaline
initial
conditions,
while
planktonic
cells
cannot.
We
associate
behavior
dynamic
interplay
between
acetate
acetoin
biosynthesis
show
mechanism
required
buffer
against
biofilm
Furthermore,
buffering-deficient
exhibit
dysregulated
development
grown
conditions.
Our
findings
reveal
could
lead
new
targets
control
unwanted
growth.
IMPORTANCE
known
influence
microbial
growth
community
dynamics
multiple
bacterial
species
environmental
contexts.
species,
rapid
demands
use
of
overflow
metabolism,
which
often
result
However,
case
communities
biofilms,
acidification
media
are
employed
stabilize
environment
for
optimal
reveals
mitigate
both
growth-associated
external
challenges.
This
discovery
provides
opportunities
understanding
methods
controlling
outside
Environmental Science Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Nanobioremediation
systems
harness
microbial
versatility
with
synthetic
and
natural
nanomaterials
to
efficiently
remove
hazardous
contaminants,
offering
a
sustainable
effective
approach
through
novel
technologies
for
environmental
protection.
Current Biology,
Journal Year:
2023,
Volume and Issue:
33(24), P. 5404 - 5414.e9
Published: Nov. 28, 2023
Cellular
responses
to
environmental
changes
are
often
highly
heterogeneous
and
exhibit
seemingly
random
dynamics.
The
astonishing
insight
of
chaos
theory
is
that
such
unpredictable
patterns
can,
in
principle,
arise
without
the
need
for
any
processes,
i.e.,
purely
deterministically
noise.
However,
while
well
understood
mathematics
physics,
its
role
cell
biology
remains
unclear
because
complexity
noisiness
biological
systems
make
testing
difficult.
Here,
we
show
explains
response
Escherichia
coli
cells
oxidative
stress.
We
developed
a
theoretical
model
gene
expression
dynamics
demonstrate
chaotic
behavior
arises
from
rapid
molecular
feedbacks
coupled
with
growth
cell-cell
interactions.
Based
on
predictions,
then
designed
single-cell
experiments
can
shift
periodic
oscillations
demand.
Our
work
suggests
regulation
be
employed
by
populations
generate
strong
variable
changing
environments.
Trends in Microbiology,
Journal Year:
2024,
Volume and Issue:
32(10), P. 944 - 956
Published: March 4, 2024
Many
pathogens
are
hard
to
eradicate,
even
in
the
absence
of
genetically
detectable
antimicrobial
resistance
mechanisms
and
despite
proven
antibiotic
susceptibility.
The
fraction
clonal
bacteria
that
temporarily
elude
effective
treatments
is
commonly
known
as
'antibiotic
persisters.'
Over
past
decade,
there
has
been
a
growing
body
research
highlighting
pivotal
role
played
by
cellular
host
development
persisters.
In
parallel,
this
also
sought
elucidate
molecular
underlying
formation
intracellular
persisters
demonstrated
prominent
for
bacterial
stress
response.
However,
questions
remain
regarding
conditions
leading
stress-induced
among
population
an
ostensibly
uniform
environment.
opinion,
following
brief
review
current
state
knowledge
persisters,
we
explore
ways
which
macrophage
functional
heterogeneity
phenotypic
may
contribute
emergence
these
We
propose
degree
mismatch
between
permissiveness
preparedness
invade
thrive
intracellularly
explain
nonreplicating
Environment International,
Journal Year:
2025,
Volume and Issue:
unknown, P. 109385 - 109385
Published: April 1, 2025
Antibiotic
resistance
genes
(ARGs)
pose
a
significant
threat
to
human
health
and
the
environment.
Quantifying
efficiency
of
horizontal
gene
transfer
(HGT)
is
challenging
due
diverse
biological
environmental
influences.
Single-cell
level
approaches
are
well-suited
for
investigating
conjugative
transfer,
given
its
reliance
on
cell-to-cell
contact
nature
capacity
offer
insights
into
population-level
responses.
This
study
introduces
self-developed
system
automated
time-lapse
image
acquisition
analysis.
Using
custom
dual-chamber
microfluidic
chip
Python-based
analysis
pipeline,
we
dynamically
quantify
ARGs
conjugation
at
single-cell
level.
By
combining
experiments
with
individual-based
modelling,
isolate
effects
subinhibitory
antibiotic
concentrations
from
those
related
bacterial
growth
dynamics.
No
variation
in
Escherichia
coli
was
observed
across
kanamycin
(0
50
mg
l-1).
Moreover,
recipient
cells
higher
rates
show
greater
propensity
plasmid
acquisition,
suggesting
physiological
state
pre-conjugation
influences
their
susceptibility
transfer.
Our
methodology
eliminates
population
bias,
revealing
intrinsic
efficiency.
approach
advances
our
understanding
factors
influencing
HGT
holds
promise
studying
other
microbial
interactions.
SYNOPSIS:
employs
reveal
that
antibiotics
affect
by
modulating
rate
rather
than