Nanoparticles-based therapeutics for the management of bacterial infections: A special emphasis on FDA approved products and clinical trials

European Journal of Pharmaceutical Sciences, Journal Year: 2023, Volume and Issue: 188, P. 106515 - 106515

Published: July 2, 2023

Microbial resistance has increased in recent decades as a result of the extensive and indiscriminate use antibiotics. The World Health Organization listed antimicrobial one ten major global public health threats 2021. In particular, six bacterial pathogens, including third-generation cephalosporin-resistant Escherichia coli, methicillin-resistant Staphylococcus aureus, carbapenem-resistant Acinetobacter baumannii, Klebsiella pneumoniae, Streptococcus Pseudomonas aeruginosa, were found to have highest resistance-related death rates 2019. To respond this urgent call, creation new pharmaceutical technologies based on nanoscience drug delivery systems appears be promising strategy against microbial light advancements, particularly knowledge medicinal biology. Nanomaterials are often defined substances having sizes between 1 100 nm. If material is used small scale; its properties significantly change. They come variety forms help provide distinguishing characteristics for wide range functions. field sciences demonstrated strong interest numerous nanotechnology applications. Therefore, review, prospective nanotechnology-based therapeutics management infections with multiple medication critically examined. Recent developments these innovative treatment techniques described, an emphasis preclinical, clinical, combinatorial approaches.

Language: Английский

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Comparative genomics reveals genetic diversity and differential metabolic potentials of the species of Arachnia and suggests reclassification of Arachnia propionica E10012 (=NBRC_14587) as novel species

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(4)

Published: March 18, 2025

Language: Английский

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Bacterial Persister Cells and Development of Antibiotic Resistance in Chronic Infections: An Update

British Journal of Biomedical Science, Journal Year: 2024, Volume and Issue: 81

Published: Aug. 7, 2024

The global issue of antimicrobial resistance poses significant challenges to public health. World Health Organization (WHO) has highlighted it as a major health threat, causing an estimated 700,000 deaths worldwide. Understanding the multifaceted nature antibiotic is crucial for developing effective strategies. Several physiological and biochemical mechanisms are involved in development resistance. Bacterial cells may escape bactericidal actions drugs by entering physiologically dormant state known bacterial persistence. Recent findings this field suggest that persistence can be one main sources chronic infections. tolerance developed persister could tolerate high levels antibiotics give rise offspring. These offspring attributed mechanisms, especially This review attempts shed light on persister-induced current therapeutic

Language: Английский

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Active pH regulation facilitates Bacillus subtilis biofilm development in a minimally buffered environment

mBio, Journal Year: 2024, Volume and Issue: 15(3)

Published: Feb. 13, 2024

ABSTRACT Biofilms provide individual bacteria with many advantages, yet dense cellular proliferation can also create intrinsic metabolic challenges including excessive acidification. Because such pH stress be masked in buffered laboratory media—such as MSgg commonly used to study Bacillus subtilis biofilms—it is not always clear how biofilms cope minimally natural environments. Here, we report B. overcome this challenge through an active regulation mechanism. Specifically, find that these modulate their extracellular the preferred neutrophile range, even when starting from acidic and alkaline initial conditions, while planktonic cells cannot. We associate behavior dynamic interplay between acetate acetoin biosynthesis show mechanism required buffer against biofilm Furthermore, buffering-deficient exhibit dysregulated development grown conditions. Our findings reveal could lead new targets control unwanted growth. IMPORTANCE known influence microbial growth community dynamics multiple bacterial species environmental contexts. species, rapid demands use of overflow metabolism, which often result However, case communities biofilms, acidification media are employed stabilize environment for optimal reveals mitigate both growth-associated external challenges. This discovery provides opportunities understanding methods controlling outside

Language: Английский

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Nanobiotechnology approaches for the remediation of persistent and emerging organic pollutants: strategies, interactions, and effectiveness

Environmental Science Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Nanobioremediation systems harness microbial versatility with synthetic and natural nanomaterials to efficiently remove hazardous contaminants, offering a sustainable effective approach through novel technologies for environmental protection.

Language: Английский

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Chaos in a bacterial stress response

Current Biology, Journal Year: 2023, Volume and Issue: 33(24), P. 5404 - 5414.e9

Published: Nov. 28, 2023

Cellular responses to environmental changes are often highly heterogeneous and exhibit seemingly random dynamics. The astonishing insight of chaos theory is that such unpredictable patterns can, in principle, arise without the need for any processes, i.e., purely deterministically noise. However, while well understood mathematics physics, its role cell biology remains unclear because complexity noisiness biological systems make testing difficult. Here, we show explains response Escherichia coli cells oxidative stress. We developed a theoretical model gene expression dynamics demonstrate chaotic behavior arises from rapid molecular feedbacks coupled with growth cell-cell interactions. Based on predictions, then designed single-cell experiments can shift periodic oscillations demand. Our work suggests regulation be employed by populations generate strong variable changing environments.

Language: Английский

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The macrophage–bacterium mismatch in persister formation

Trends in Microbiology, Journal Year: 2024, Volume and Issue: 32(10), P. 944 - 956

Published: March 4, 2024

Many pathogens are hard to eradicate, even in the absence of genetically detectable antimicrobial resistance mechanisms and despite proven antibiotic susceptibility. The fraction clonal bacteria that temporarily elude effective treatments is commonly known as 'antibiotic persisters.' Over past decade, there has been a growing body research highlighting pivotal role played by cellular host development persisters. In parallel, this also sought elucidate molecular underlying formation intracellular persisters demonstrated prominent for bacterial stress response. However, questions remain regarding conditions leading stress-induced among population an ostensibly uniform environment. opinion, following brief review current state knowledge persisters, we explore ways which macrophage functional heterogeneity phenotypic may contribute emergence these We propose degree mismatch between permissiveness preparedness invade thrive intracellularly explain nonreplicating

Language: Английский

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The master regulator OxyR orchestrates bacterial oxidative stress response genes in space and time

Cell Systems, Journal Year: 2024, Volume and Issue: 15(11), P. 1033 - 1045.e6

Published: Nov. 1, 2024

Bacteria employ diverse gene regulatory networks to survive stress, but deciphering the underlying logic of these complex has proved challenging. Here, we use time-resolved single-cell imaging explore functioning E. coli response oxidative stress. We observe expression dynamics within network. However, by controlling for stress-induced growth-rate changes, show that patterns involve just three classes regulation: downregulated genes, upregulated pulsatile and gradually genes. The two are distinguished differences in binding transcription factor, OxyR, appear play distinct roles during stress protection. Pulsatile genes activate transiently a few cells initial protection group cells, whereas induce evenly, generating lasting involving many cells. Our study shows how bacterial populations simple principles coordinate responses space time. A record this paper's transparent peer review process is included supplemental information.

Language: Английский

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Adaptive responses of Alicyclobacillus acidoterrestris in acidic broth and fruit juices: Focus on the influences of organic acids and temperature conditions

International Journal of Food Microbiology, Journal Year: 2025, Volume and Issue: 430, P. 111058 - 111058

Published: Jan. 6, 2025

Language: Английский

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Single-cell analysis reveals antibiotic affects conjugative transfer by modulating bacterial growth rather than conjugation efficiency

Environment International, Journal Year: 2025, Volume and Issue: unknown, P. 109385 - 109385

Published: April 1, 2025

Antibiotic resistance genes (ARGs) pose a significant threat to human health and the environment. Quantifying efficiency of horizontal gene transfer (HGT) is challenging due diverse biological environmental influences. Single-cell level approaches are well-suited for investigating conjugative transfer, given its reliance on cell-to-cell contact nature capacity offer insights into population-level responses. This study introduces self-developed system automated time-lapse image acquisition analysis. Using custom dual-chamber microfluidic chip Python-based analysis pipeline, we dynamically quantify ARGs conjugation at single-cell level. By combining experiments with individual-based modelling, isolate effects subinhibitory antibiotic concentrations from those related bacterial growth dynamics. No variation in Escherichia coli was observed across kanamycin (0 50 mg l-1). Moreover, recipient cells higher rates show greater propensity plasmid acquisition, suggesting physiological state pre-conjugation influences their susceptibility transfer. Our methodology eliminates population bias, revealing intrinsic efficiency. approach advances our understanding factors influencing HGT holds promise studying other microbial interactions. SYNOPSIS: employs reveal that antibiotics affect by modulating rate rather than

Language: Английский

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