
Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown
Published: March 25, 2025
ABSTRACT Hydrogen sulfide is produced during the dissolution of some sulfidic minerals and microbial metabolism reduced sulfur compounds. The sulfide:quinone reductase (SQR) enzyme able to oxidize H 2 S, bioleaching cells Acidithiobacillus ferrooxidans have two SQR genes, only one which has been characterized. We cloned overexpressed genes in A. show that they both activity. Both AFE_0267 AFE_1792 are active under anaerobic conditions, but aerobic conditions. effect overexpression expression related on was investigated. improved cell growth oxidation, suggesting enhanced activity led a reduction S toxicity as well providing additional energy through oxidation. Additionally, impact oxidation pyrite pyrrhotite rate by engineered enhanced, and, furthermore, more than doubled. IMPORTANCE toxic intermediate, evolved . In addition detoxification, provides energy, sulfur. also may facilitate pyrometallurgical processing number critical materials including copper, nickel, platinum group metals.
Language: Английский