Survival and Spread of EngineeredMycobacterium smegmatisand Associated Mycobacteriophage in Soil Microcosms

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract The inoculation of microbes into soil environments has numerous applications for improving quality and crop health; however, the ability exogenous engineered to survive spread in remains uncertain. To address this challenge, we assayed survival Mycobacterium smegmatis , with either plasmid transformation or genome integration, as well its mycobacteriophage Kampy, both sterilized non-sterilized microcosms over a period 49 days. While M. Kampy persisted all microcosms, there was minimal evidence 5 cm away from site. There higher prevalence observed than soil, suggesting detrimental effect native biotic viral community on phage proliferate microcosm. Additionally, abundance genome-integrated bacteria relative plasmid-carrying loss duration experiment suggest burden associated harboring plasmids, although plasmids were still retained across our knowledge, is first study simultaneously measure persistence their employing plasmid-based circuits. As such, provides novel understanding challenges deployment bioengineered environments. Importance Healthy essential sustain life, it habitable land, enables food production, promotes biodiversity, sequesters cycles nutrients, filters water. Given degradation, treatment that promote health could improve global sustainability; furthermore, application bioengineering synthetic biology these allows fine-tunable robust control gene-of-interest expression. These solutions require successful an environment which abundant competition often limited nutrients can result bacterial death dormancy. This employs chassis alongside bacteriophage assess non-native soil. Insights highlight important must be overcome field.

Language: Английский

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Root Exudate Lipids: Uncovering Chemodiversity and Carbon Stability Potential

Soil Biology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109799 - 109799

Published: April 1, 2025

Language: Английский

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A roadmap to understanding and anticipating microbial gene transfer in soil communities

Microbiology and Molecular Biology Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

SUMMARY Engineered microbes are being programmed using synthetic DNA for applications in soil to overcome global challenges related climate change, energy, food security, and pollution. However, we cannot yet predict gene transfer processes assess the frequency of unintentional engineered environmental when applying biology technologies at scale. This challenge exists because complex heterogeneous characteristics soils, which contribute fitness transport cells exchange genetic material within communities. Here, describe knowledge gaps about across microbiomes. We propose strategies improve our understanding communities, highlight need benchmark performance biocontainment measures situ , discuss responsibly engaging community stakeholders. opportunities address gaps, such as creating a set standards studying diverse types measuring host range microbiomes emerging technologies. By comparing rates, range, persistence different posit that community-scale, environment-specific models can be built anticipate biotechnology risks. Such studies will enable design safer biotechnologies allow us realize benefits mitigate risks associated with release

Language: Английский

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Integrating ‘cry for help’ strategies for sustainable agriculture

Trends in Plant Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Editorial overview: Plant synthetic biology

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 90, P. 103211 - 103211

Published: Sept. 27, 2024

Language: Английский

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