Leveraging Engineered Pseudomonas putida Minicells for Bioconversion of Organic Acids into Short-Chain Methyl Ketones

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Methyl ketones, key building blocks widely used in diverse industrial applications, largely depend on oil-derived chemical methods for their production. Here, we investigated biobased production alternatives short-chain adapting the solvent-tolerant soil bacterium Pseudomonas putida as a host ketone biosynthesis either by whole-cell biocatalysis or using engineered minicells, chromosome-free bacterial vesicles. Organic acids (acetate, propanoate and butanoate) were selected main carbon substrate to drive of acetone, butanone 2-pentanone. Pathway optimization identified efficient enzyme variants from Clostridium acetobutylicum Escherichia coli, tested with both constitutive inducible expression cognate genes. By implementing these optimized pathways P. which can be prepared through simple three-step purification protocol, feedstock was converted into target methyl ketones. These results highlight value combining morphology pathway engineering noncanonical hosts establish alternative bioprocesses toxic chemicals that are difficult produce conventional approaches.

Language: Английский

Editorial overview: Chemical biotechnology paving the way for a sustainable future

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 90, P. 103215 - 103215

Published: Sept. 28, 2024

Language: Английский